Elevated Level of Circulating Platelet-derived Microparticles in Oral Cancer

Abstract

Numerous studies have demonstrated that circulating microparticles (MPs) play important roles in a variety of diseases (e.g., atherosclerosis, hypertension, and diabetes), but the association between circulating MPs and oral squamous cell carcinoma (OSCC) remains largely unknown. In the present study, the circulating platelet-derived MPs (PMPs) in 63 patients with OSCC, 22 patients with infected keratocystic odontogenic tumor, and 31 healthy volunteers were characterized and quantified by flow cytometric analysis. The coagulation function of patients with OSCC was correspondingly evaluated. Meanwhile, the inflammation-related cytokines were detected in plasma by enzyme-linked immunosorbent assay and in tumor tissues by immunohistochemistry. Our results showed that the plasma level of circulating PMPs was significantly higher in OSCC patients compared with healthy volunteers and patients with infected keratocystic odontogenic tumor, and they showed positive correlation with the increased level of fibrinogen. Moreover, the coagulation time was significantly shorter after the MPs were added to the MP-free plasma. Most important, the levels of interleukin 6 and tumor necrosis factor α in plasma and tumor tissues were significantly increased in OSCC patients, which were closely correlated with the elevated level of circulating PMPs. In summary, this study suggests that the elevated level of circulating PMPs, showing close correlation with the secretion of inflammation-related factors, may contribute to the increased procoagulant activity in patients with OSCC.

Clinical Significance and Roles in Angiogenesis of Circulating Microparticles in Oral Cancer

“…Our previous study demonstrated that elevated levels of circulating MPs were closely correlated with the secretion of inflammation-related cytokines and so might be a cause of increased procoagulant activity in patients with OSCC (Ren et al 2016). However, the changes in different phenotypes of circulating MPs in OSCC are still to be clarified.…”

“…Circulating MPs were isolated and detected according to our previous study (Ren et al 2016). Briefly, 5 mL of venous citrated blood was collected and centrifuged at 1,550 g for 20 min.…”

Role of Platelet-Derived Microvesicles As Crosstalk Mediators in Atherothrombosis and Future Pharmacology Targets: A Link between Inflammation, Atherosclerosis, and Thrombosis

“…Raised levels of circulating pMVs have been detected in young women with polycystic ovary syndrome (Willis et al, 2014), in patients with rheumatoid arthritis in which pMVs express CLEC-2 (Gitz et al, 2014) and in patients with antiphospholipid antibodies in which pMVs correlate with anti-β2-GP (Chaturvedi et al, 2015). Additionally, elevated levels of circulating pMVs contribute to the increase PCA in patients with oral cancer (Ren et al, 2015). …”

Characterization of microparticles in patients with venous malformations of the head and neck

“…MP were isolated from peripheral venous blood (circulating MP) or lesional fluid from VM patients (lesional MP) as similar to our previous studies (Ren et al , ,b). Briefly, venous blood (5 ml) was collected on citrated tubes from VM patients or healthy volunteers while lesional fluid was drawn from VM patients.…”