Elevated levels of anti‐endotoxin antibodies in patients with bilateral idiopathic acute anterior uveitis

Misiuk-Hojło, Marta; Międzybrodzki, Ryszard; Grzybowski, Andrzej; Ługowski, Czesław; Niedziela, Tomasz; Turno-Kręcicka, Anna; Szymaniec, S

doi:10.1111/j.1755-3768.2010.01969.x

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…[4][5][6] After injecting lipopolysaccharide (LPS) through the footpad intraperitoneally, intravenously, or intravitreally, the breakdown of the blood-aqueous barrier bilaterally, and polymorphonuclear leukocyte and mononuclear cell infiltration in the iris and ciliary body, and in the anterior chamber are noted within 24 hours. The intraocular inflammation subsides after 96 hours.…”

Section: Methods Eiu Was Induced By Footpad Injection Of Lps Intomentioning

confidence: 99%

Anti-Inflammatory Effect of the Proteasome Inhibitor Bortezomib on Endotoxin-Induced Uveitis in Rats

Chen

Liu

Yang

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. We evaluated the anti-inflammatory effect of bortezomib (Velcade), a proteasome inhibitor, on endotoxin-induced uveitis (EIU) in rats and lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. METHODS. EIU was induced by footpad injection of LPS intoLewis rats. MG-132 (10 mg/kg), or high-dose (0.2 mg/kg) or low-dose (0.05 mg/kg) bortezomib was given 30 minutes before LPS injection in each treatment group. The rats were sacrificed 24 hours later to observe the inflammatory response in tissues. The expression levels of fractalkine, MCP-1, ICAM-1, and iNOS were evaluated by PCR and Western blot analysis. Immunohistochemical (IHC) studies were used to demonstrate the expression of pro-inflammatory mediators and nuclear factor-kappa B (NF-jB) p65 in the iris and ciliary body. The DNA-binding activity of NF-jB was evaluated using an electrophoretic mobility shift assay (EMSA). An in vitro study using RAW 264.7 cells was performed to verify the results.RESULTS. Pretreatment with high-dose bortezomib significantly attenuated the inflammatory response of EIU. Reduced expression of inflammatory mediators always was observed in the high-dose bortezomib and MG-132 groups, but invariably was not noted in the low-dose bortezomib group. Decreased DNA-binding activity of NF-jB was noted in those rats pretreated with high-dose bortezomib or MG-132. In vitro study demonstrated the dose-dependent anti-inflammatory effects of bortezomib in LPS-stimulated RAW cells, consistent with the results obtained in vivo.CONCLUSIONS. Bortezomib inhibits EIU, probably by inhibiting the activation of NF-jB, which in turn, down-regulates the expression of the associated inflammatory genes. Proteasome inhibition may be a potential treatment strategy for uveitis. (Invest Ophthalmol Vis Sci. 2012;53:3682-3694)

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Section: Methods Eiu Was Induced By Footpad Injection Of Lps Intomentioning

confidence: 99%

Anti-Inflammatory Effect of the Proteasome Inhibitor Bortezomib on Endotoxin-Induced Uveitis in Rats

Chen

Liu

Yang

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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“…Bilateral simultaneous disease is much less common and behaves differently from unilateral disease, with fewer recurrences. 15 In our series, HLA-B27 was not a common etiology of BAAU; only 5 of 35 patients tested (14%) were positive for the allele. Two of these patients had postinfectious or drug-induced uveitis, and one was classified as having TINU syndrome.…”

Section: Commentmentioning

confidence: 44%

“…Antibodies targeting endotoxin lipopolysaccharides of gram-negative bacteria have been found to be elevated in patients positive for HLA-B27 during their first attack of anterior acute uveitis, relative to levels during relapses. 15 Patients with bilateral disease had higher antibody levels but lower recurrence rates than patients with unilateral disease. 18 This is in keeping with our finding that the HLA-B27-positive patient with acute disease and 15 months of follow-up did not have a relapse.…”

Section: Commentmentioning

confidence: 94%

Bilateral Simultaneous-Onset Nongranulomatous Acute Anterior Uveitis

Birnbaum¹,

Jiang

Vasaiwala

et al. 2012

Arch Ophthalmol

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OBJECTIVE To describe the etiology and outcome of patients with simultaneous-onset nongranulomatous bilateral acute anterior uveitis. METHODS The medical records of patients who presented to a single tertiary care center with simultaneous-onset nongranulomatous bilateral acute anterior uveitis between January 1990 and May 2010 were retrospectively reviewed; the clinical presentation, results of diagnostic testing, and outcome data are described. RESULTS A total of 4288 new patients with uveitis were evaluated by the Uveitis Service at the University of Illinois at Chicago Eye and Ear Infirmary between January 1990 and May 2010. Of these new patients, 44 (1%) presented with simultaneous-onset nongranulomatous bilateral acute anterior uveitis. The most common etiologies were postinfectious or drug-induced uveitis (23 of 44 patients [52%]) and idiopathic uveitis (15 patients [34%]). Tubulointerstitial nephritis and uveitis syndrome, HLA-B27-associated uveitis, inflammatory bowel disease, and Kawasaki disease each made up fewer than 5% of diagnoses. Overall, this group of patients was younger than the entire cohort of new patients with uveitis who were evaluated during the same time period (P = .002). For 14 of the 15 patients with at least a year of follow-up (93%), the disease duration was limited (<3 months). Of these 14 patients, 7 (50%) developed recurrent disease, with an average time to first recurrence of 20 months (range, 7.5-40 months) after resolution of the initial inflammatory episode. CONCLUSIONS Simultaneous-onset nongranulomatous bilateral acute anterior uveitis is a rare clinical entity that is more common in younger patients and is most frequently associated with recent infection and/or systemic antibiotic use. Tubulointerstitial nephritis and uveitis syndrome should also be considered as a diagnosis. Diagnostic evaluation should include serum antistreptolysin-O titers, HLA-B27 antigen, and urine β2 microglobulin levels because these may reveal systemic disease that requires therapy.

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“…Similarly, in humans, the majority of individuals who are HLA-B27-positive do not develop AAU or other autoimmune diseases, implying the involvement of other genes or environmental factors in the development of uveitis. Some literature suggests the involvement of microbial triggers in disease development, in particular Gram-negative bacteria ( 114 , 115 ).…”

Section: Microbial Pathogens and Host Factors In Intraocular Inflammamentioning

confidence: 99%

Ocular Microbiota and Intraocular Inflammation

Wei

2020

Front. Immunol.

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The term ocular microbiota refers to all types of commensal and pathogenic microorganisms present on or in the eye. The ocular surface is continuously exposed to the environment and harbors various commensals. Commensal microbes have been demonstrated to regulate host metabolism, development of immune system, and host defense against pathogen invasion. An unbalanced microbiota could lead to pathogenic microbial overgrowth and cause local or systemic inflammation. The specific antigens that irritate the deleterious immune responses in various inflammatory eye diseases remain obscure, while recent evidence implies a microbial etiology of these illnesses. The purpose of this review is to provide an overview of the literature on ocular microbiota and the role of commensal microbes in several eye diseases. In addition, this review will also discuss the interaction between microbial pathogens and host factors involved in intraocular inflammation, and evaluate therapeutic potential of targeting ocular microbiota to treat intraocular inflammation.

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Elevated levels of anti‐endotoxin antibodies in patients with bilateral idiopathic acute anterior uveitis

Cited by 5 publications

References 40 publications

Anti-Inflammatory Effect of the Proteasome Inhibitor Bortezomib on Endotoxin-Induced Uveitis in Rats

Anti-Inflammatory Effect of the Proteasome Inhibitor Bortezomib on Endotoxin-Induced Uveitis in Rats

Bilateral Simultaneous-Onset Nongranulomatous Acute Anterior Uveitis

Ocular Microbiota and Intraocular Inflammation

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