Elevated levels of DNA-protein crosslinks and micronuclei in peripheral lymphocytes of tannery workers exposed to trivalent chromium

Medeiros, M.G.; Rodrigues, António Sebastião; Batoréu, M.C.; Laires, A.; Rueff, José; Zhitkovich, Anatoly

doi:10.1093/mutage/18.1.19

Cited by 97 publications

(55 citation statements)

References 43 publications

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“…Chromium was reported to be a genotoxic carcinogen affecting the fragile site, a heritable point on the chromosome where gaps and breaks tend to occur. The results obtained in this study might be strongly supported by the detectable increase in lymphocyte DNA damage due to chronic occupational exposure to Cr-III which correlated significantly with exposure of the cells to the metal [36] . Although the precise mechanisms remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity [8] .…”

Section: Discussionsupporting

confidence: 74%

“…However, in another study, no significant difference was observed between exposed and controls workers exposed to chromium with regard to the frequency of peripheral lymphocytes with chromosome aberrations [35] . Nowadays, DNA-protein cross-links are considered a promising biomarker signaling an exposure of body tissues to biologically active forms of chromium [36] .…”

Section: Discussionmentioning

confidence: 99%

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The Role of Cd40 Ligand in Early Detection of Chromium-Induced Immunotoxic Effects Among Exposed Workers

Rashed

2007

Egypt. J. Occup. Med.

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Objective: the immunological effect of chromium exposure among workers in tanning industry was assessed by studying the incidence of structural chromosomal aberrations in lymphocytes. Early monitoring of immunotoxicity and possibility of carcinogenecity using the CD40 ligand member of the tumor necrosis superfamily of trans-membrane proteins was evaluated.Methods: levels of chromium in blood (S-Cr) and urine (U-Cr) were measured using the graphite furnace atomic absorption spectrophotometer. The CD40 Ligand (CD40L) was determined using ELISA. Whole blood was treated and chosen metaphases were recorded and scored for morphological aberrations and rearrangements.Results: the study was carried out on 30 workers exposed to chromium in chrome-tanning for 17.13 ± 8.09 years, and a matched group of 28 administrative workers. The mean serum chromium levels (S-Cr) were 4.35 ± 1.46 and 1.89 ± 1.31 µg/dl among exposed and control groups, respectively, and the difference was statistically significant, P<0.00±. The CD40L assessment revealed decreased levels among the exposed population, difference was statistically non-significant. The genotoxic changes in the form of chromosomal and chromatid gaps and breaks as well as dicen-237

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

The Role of Cd40 Ligand in Early Detection of Chromium-Induced Immunotoxic Effects Among Exposed Workers

Rashed

2007

Egypt. J. Occup. Med.

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“…The fumes from industrial stainless steel welding processes also increased CA in 11 welders [Borska et al, 2003]. As part of a large study on tannery workers, Medeiros et al [2003] reported that five welders had a higher frequency of DNA-protein crosslinks than the control group. However, probably due to the small number of welders involved in this study, the authors reported no increase in the frequency of micronuclei for these individuals.…”

Section: Introductionmentioning

confidence: 99%

“…As indicated above, biological endpoints, such as DNA damage, can be monitored by analysis of CA and SCE [Jelmert et al, 1995], micronuclei in either buccal cells [Danadevi et al, 2004] or lymphocytes [Medeiros et al, 2003], DNA-protein crosslinks [Costa et al, 1993], or unscheduled DNA synthesis [Knudsen et al, 1992]. In addition, the alkaline Comet assay is a rapid and sensitive technique to assess DNA strand breaks and alkali-labile sites in individual cells.…”

Section: Introductionmentioning

confidence: 99%

Assessment of occupational exposure to welding fumes by inductively coupled plasma‐mass spectroscopy and by the alkaline Comet assay

Botta¹,

Iarmarcovai²,

Chaspoul³

et al. 2006

Environ and Mol Mutagen

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Welding fumes are classified as possibly carcinogenic to humans (Group 2B) by the International Agency for Research on Cancer. In the current study, blood and urine concentrations of aluminum (Al), cadmium (Cd), cobalt (Co), chromium (Cr), manganese (Mn), nickel (Ni), lead (Pb), and zinc (Zn) were monitored by inductively coupled plasma-mass spectrometry (ICP-MS) in 30 welders and in 22 controls. In addition, DNA damage was examined in the lymphocytes of these subjects by the alkaline Comet assay. Two biological samples were taken from the welders at the beginning (BW) and at the end (EW) of a work week. In controls, collection of samples was limited to BW. Blood concentrations of Cd, Co, Cr, Ni, and Pb were higher in the welders than in the control group while higher concentrations of Al, Cd, Co, Cr, Ni, and Pb were detected in welder urines.There was no significant difference in the metal concentrations for the BW and EW welder samples. Increased levels of DNA damage were found in lymphocytes from welders as compared to the controls, and 20/30 welders had higher levels of DNA lesions in the EW than in the BW samples. Age had a significant effect on DNA damage in the control group. Spearman's rank correlation analysis indicated that there were positive correlations between blood concentrations of Al, Co, Ni, and Pb and the levels of DNA damage. A negative correlation was found between DNA damage and Mn in blood, while there was a positive correlation between urinary Mn concentration and DNA damage. These data indicate that occupational exposure to welding fumes increases DNA damage in lymphocytes. Environ. Mol. Mutagen. 47:284-295, 2006. V V C 2006 Wiley-Liss, Inc.

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“…Of importance from a practical standpoint, SCEs appear to result only as an effect of chemical mutagens, not radiation. They are most efficiently induced by substances that form covalent adducts to the DNA, distort the DNA helix, or interfere with DNA precursor metabolism or repair (Medeiros et al, 2003).…”

Section: Sister Chromatid Exchangementioning

confidence: 99%

Occupational Exposure Gene Interaction : Review Article

2013

Egypt. J. Occup. Med.

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The lack of knowledge about the earliest events in occupational disease development is due to the multi-factorial nature of disease risk. This information gap is the consequence of the lack of appreciation for the fact that most occupational diseases arise from the complex interactions between genes and the occupational exposure. Whether an occupational exposure causes illness or not is dependent on the efficiency of metabolic pathways. Thus, elucidating the causes of most chronic diseases will require an understanding of both the genetic and occupational contribution to their etiology. Unfortunately, the exploration of the relationship between genes and the occupational exposure has been hampered in the past by the limited knowledge of the human genome, and by the inclination of scientists to study disease development using experimental models that consider exposure to a single environmental agent in the workplace. To understand how genes and occupational agents interact to initiate biological pathways to cause injury or disease, scientists will need tools with the capacity to monitor the global expression of thousands of genes, proteins and metabolites simultaneously. The generation of such data in multiple species can be used to identify conserved and functionally significant genes and pathways involved in gene environment interactions. The complex interplay between genes and occupational exposure represents also a great challenge to scientists, and it is also an important opportunity to reduce the burden of disease and dysfunctions on humans. Major technological advances in the last few years have increased our knowledge of the role that genetics has in occupational diseases and our understanding of genetic components and the interaction between genetics and environmental factors. The complex interplay between genes and occupational exposure represents also a great challenge to scientists, and it is also an important opportunity to reduce the burden of disease and dysfunctions on humans.

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Elevated levels of DNA-protein crosslinks and micronuclei in peripheral lymphocytes of tannery workers exposed to trivalent chromium

Cited by 97 publications

References 43 publications

The Role of Cd40 Ligand in Early Detection of Chromium-Induced Immunotoxic Effects Among Exposed Workers

The Role of Cd40 Ligand in Early Detection of Chromium-Induced Immunotoxic Effects Among Exposed Workers

Assessment of occupational exposure to welding fumes by inductively coupled plasma‐mass spectroscopy and by the alkaline Comet assay

Occupational Exposure Gene Interaction : Review Article

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