Elevated levels of IL-8 in dengue hemorrhagic fever

Raghupathy, Raj; Chaturvedi, U. C.; Al‐Sayer, H.; Elbishbishi, E.A.; Agarwal, Rajeev; Nagar, Rachna; Kapoor, Sanjay; Misra, A.; Mathur, Asha; Nusrat, Hamida; Azizieh, Fawaz; Khan, Mohsin; Mustafa, Abu Salim

doi:10.1002/(sici)1096-9071(199811)56:3<280::aid-jmv18>3.0.co;2-i

Cited by 149 publications

(127 citation statements)

References 27 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that the levels of tumor necrosis factor ␣ (TNF-␣), IL-6, and IL-8 but not IL-1␤ in patients with DHF/ DSS were relatively higher than those of healthy persons. 16,20,21 TNF-␣ production by endothelial cells has not been reported. However, TNF-␣ released from DV-infected monocytes could modulate endothelial cell function by an indirect route.…”

Section: Ribavirin Inhibits Dv-induced Il-6 and Il-8 Production By Humentioning

confidence: 97%

Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.

Huang¹,

Lei²,

Liu³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

158

148

View full text Add to dashboard Cite

Abstract. In this study dengue virus (DV) was found to infect primary endothelial cells derived from human umbilical cord veins (HUVEC) and alter their cytokine production. Dengue virus infection of HUVEC was confirmed by an increase in plaque-forming units in the culture supernatant and by immunofluorescence assay. HUVEC produced large amounts of interleukin (IL)-6 and IL-8 but not IL-1␤ after DV infection. Both the replication of DV and the production of IL-6 and IL-8 by HUVEC after DV infection were inhibited by ribavirin, an antiviral synthetic guanosine analogue. Additionally, increased serum levels of IL-6 and IL-8 were observed in patients with dengue hemorrhagic fever but not dengue fever. Therefore, our results suggest that endothelial cells can be a target for DV infection, and that DV-induced IL-6 and IL-8 production by endothelial cells may contribute to the pathogenesis of dengue hemorrhagic fever.

show abstract

Section: Ribavirin Inhibits Dv-induced Il-6 and Il-8 Production By Humentioning

confidence: 97%

Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.

Huang¹,

Lei²,

Liu³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

158

148

View full text Add to dashboard Cite

show abstract

“…D2V-infected cell-culture medium and IL8 induce alterations in the actin cytoskeleton and focal adhesions of control monolayers Actin reorganization observed in non-infected cells neighbouring infected ones in the same monolayer after 48 h of infection suggested the presence of chemical mediators such as IL8 and TNF-a, because these cytokines have been proposed to be inductors of actin rearrangements (Blum et al, 1997;Schraufstatter et al, 2001). Furthermore, levels of IL8 have been found to be elevated in sera and pleural fluid of patients with DHF (Raghupathy et al, 1998), and it has also been detected in DV-infected HUVEC (Bosch et al, 2002). MicroELISA assays revealed the presence of increasing concentrations of IL8 in the culture medium of virusinfected monolayers.…”

Section: Actin Cytoskeleton Rearrangements and Occludin Redistributiomentioning

confidence: 99%

IL8 release, tight junction and cytoskeleton dynamic reorganization conducive to permeability increase are induced by dengue virus infection of microvascular endothelial monolayers

Talavera

Castillo

Domı́nguez

et al. 2004

Journal of General Virology

183

152

View full text Add to dashboard Cite

Permeability alterations of microvascular endothelia may be a factor in the plasma leakage produced by dengue virus infection. Confluent monolayers of the human dermal microvascular endothelial cell line HMEC-1 were utilized as an experimental model to study the cellular responses induced by the virus. Infected monolayers showed increased permeability for [ 3 H]mannitol, but no changes were observed for 4-70 kDa dextrans at 48 h post-infection (p.i.), a time at which viral titres reached maximal values and 40 % of the cells expressed viral proteins. A further increase in permeability occurred at 72 h, still without evident cytopathic effects on the monolayer. Coinciding with this, actin was reorganized in the infected cells and the tight junction protein occludin was displaced to the cytoplasm. Increments in the thickness of stress fibres and focal adhesions were observed in uninfected cells neighbouring infected cells. Culture medium from infected monolayers induced permeability changes and thickening of actin-containing structures in control cultures that resembled those observed 48 h p.i. Interleukin (IL) 8 was found in culture medium at concentrations ranging from 20 to 100 pg ml "1 . Neutralizing antibodies against IL8 partially inhibited the changes produced by the culture medium as well as those induced by addition of IL8. Genistein inhibited the effect of the culture medium and the phosphorylation of proteins associated with focal adhesions and indicated the participation of tyrosine kinases. These findings suggest that IL8 production by infected monolayers contributes to the virus-induced effect on the cytoskeleton and tight junctions and thereby modifies transendothelial permeability.

show abstract

“…[13][14][15][16] Other studies have suggested that cytokine levels can be used as predictive factors for severity in dengue. 17 High levels of IL-6 have implications for development of pathology in severe dengue, playing an important role in the production of antiplatelet or antiendothelial cell antibodies, as well as elevated levels of tissue plasminogen activator, and a deficiency in coagulation, leading to plasma leakage and bleeding.…”

Section: Introductionmentioning

confidence: 99%

Dengue Patients with Early Hemorrhagic Manifestations Lose Coordinate Expression of the Anti-Inflammatory Cytokine IL-10 with the Inflammatory Cytokines IL-6 and IL-8

Iani¹,

Caldas

Duarte

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Dengue is responsible for a wide range of clinical manifestations, ranging from asymptomatic infections to severe cases. The alteration of cytokine levels correlated with clinical characteristics can help determine prognostic markers of the disease and the identification of targets for immunotherapy. We measured the viral load, serotype, and cytokine levels of 212 serum samples from patients with acute dengue infection during days 1-4 after the onset of symptoms. The patients were classified as either with hemorrhagic manifestations (HM) or with no hemorrhagic manifestations (NHM). The cytokines interleukin-6 (IL-6), IL-8, and IL-10 were increased (P < 0.05) in the dengue virus+ group, compared with the control group. A higher viral load (P < 0.05) and IL-6 was detected in the HM group compared with the NHM group. Interestingly, the NHM group demonstrated a significant positive correlation between inflammatory (IL-6 and 8) and anti-inflammatory (IL-10) cytokines, whereas the HM group did not. These findings suggest that a disturbance in the balance of inflammatory cytokines IL-6 and IL-8 with the anti-inflammatory cytokine, IL-10, combined with the high levels of IL-6 and viral load, characterize possible mechanisms related to the formation of HM.

show abstract

Elevated levels of IL-8 in dengue hemorrhagic fever

Cited by 149 publications

References 27 publications

Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.

Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.

IL8 release, tight junction and cytoskeleton dynamic reorganization conducive to permeability increase are induced by dengue virus infection of microvascular endothelial monolayers

Dengue Patients with Early Hemorrhagic Manifestations Lose Coordinate Expression of the Anti-Inflammatory Cytokine IL-10 with the Inflammatory Cytokines IL-6 and IL-8

Contact Info

Product

Resources

About