Elevated levels of intrachromosomal homologous recombination in <i>Arabidopsis</i> overexpressing the <i>MIM</i> gene

Hanin, Moez; Mengiste, Tesfaye; Bogucki, Augustyn; Paszkowski, Jerzy

doi:10.1046/j.1365-313x.2000.00867.x

Cited by 53 publications

(32 citation statements)

References 40 publications

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“…defective in a calcium binding EF-hand protein) ; while mutation in BRU1 (a novel protein involved in DNA repair and replication) (Takeda et al, 2004), RAD50 (Gherbi et al, 2001), RAD17, or RAD9 (Heitzeberg et al, 2004) only slightly increases HR, whereas teb (a homolog of human DNA polymerase u) (Inagaki et al, 2006), rad51c, or rad51d (a bacterial RecA recombinase homolog) (Durrant et al, 2007), mim (hypersensitive to MMS, irradiation, and MMC, a structural maintenance of chromosome protein) (Mengiste et al, 1999;Hanin et al, 2000), and ino80 (an ortholog of the SWI/SNF ATPase family) mutations apparently decreased HR. We also observed that mutations in ROR1/RPA2A elevated the HR to a similar level as in abo4 mutants (see Supplemental Figure 2 online).…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

et al. 2009

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Based on abscisic acid (ABA) inhibition of seed germination and seedling growth assays, we isolated an ABA overly sensitive mutant (abo4-1) caused by a mutation in the Arabidopsis thaliana POL2a/TILTED1(TIL1) gene encoding a catalytic subunit of DNA polymerase «. The dominant, ABA-insensitive abi1-1 or abi2-1 mutations suppressed the ABA hypersensitivity of the abo4-1 mutant. The abo4/til1 mutation reactivated the expression of the silenced Athila retrotransposon transcriptional silent information (TSI) and the silenced 35S-NPTII in the ros1 mutant and increased the frequency of somatic homologous recombination (HR) ;60-fold. ABA upregulated the expression of TSI and increased HR in both the wild type and abo4-1. MEIOTIC RECOMBINATION11 and GAMMA RESPONSE1, both of which are required for HR and double-strand DNA break repair, are expressed at higher levels in abo4-1 and are enhanced by ABA, while KU70 was suppressed by ABA. abo4-1 mutant plants are sensitive to UV-B and methyl methanesulfonate and show constitutive expression of the G2/Mspecific cyclin CycB1;1 in meristems. The abo4-1 plants were early flowering with lower expression of FLOWER LOCUS C and higher expression of FLOWER LOCUS T and changed histone modifications in the two loci. Our results suggest that ABO4/POL2a/TIL1 is involved in maintaining epigenetic states, HR, and ABA signaling in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

et al. 2009

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“…A link between chromatin structure, gene silencing, and genome maintenance had been previously proposed for the MIM gene (Hanin et al 2000) and for BRU1, an Arabidopsis gene linking heterochromatin stability to gene silencing as well as to DNA repair (Takedaet al 2004). Our results on DDM1 provide a new example of this link for a chromatin remodeling SWI2/SNF2 gene and further support the link between genetic and epigenetic stability.…”

Section: Discussionmentioning

confidence: 99%

“…Disrupting the nucleosome-DNA interactions or remodeling of chromatin via ATP-dependent proteins might thus stimulate HR and DNA repair. In support of this possibility, it was shown that alteration in the expression of MIM, a gene encoding a chromatin structural component related to the SMC family (structure maintenance of chromosomes), could affect the rate of intrachromosomal recombination in Arabidopsis (Hanin et al 2000). BRU1 is an additional example of an Arabidopsis gene linking heterochromatin stability to gene silencing as well as to DNA repair (Takeda et al 2004).…”

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Involvement of the ArabidopsisSWI2/SNF2Chromatin Remodeling Gene Family in DNA Damage Response and Recombination

2006

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The genome of plants, like that of other eukaryotes, is organized into chromatin, a compact structure that reduces the accessibility of DNA to machineries such as transcription, replication, and DNA recombination and repair. Plant genes, which contain the characteristic ATPase/helicase motifs of the chromatin remodeling Swi2/Snf2 family of proteins, have been thoroughly studied, but their role in homologous recombination or DNA repair has received limited attention. We have searched for homologs of the yeast RAD54 gene, whose role in recombination and repair and in chromatin remodeling is well established. Forty Arabidopsis SWI2/SNF2 genes were identified and the function of a selected group of 14 was analyzed. Mutant analysis and/or RNAi-mediated silencing showed that 11 of the 14 genes tested played a role in response to DNA damage. Two of the 14 genes were involved in homologous recombination between inverted repeats. The putative ortholog of RAD54 and close homologs of ERCC6/ RAD26 were involved in DNA damage response, suggesting functional conservation across kingdoms. In addition, genes known for their role in development, such as PICKLE/GYMNOS and PIE1, or in silencing, such as DDM1, turned out to also be involved in DNA damage response. A comparison of ddm1 and met1 mutants suggests that DNA damage response is affected essentially by chromatin structure and that cytosine methylation is less critical. These results emphasize the broad involvement of the SWI2/SNF2 family, and thus of chromatin remodeling, in genome maintenance and the link between epigenetic and genetic processes.

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“…Mutants of nuclear protein BRU1 involved in the maintenance of chromatin structure were highly sensitive to genotoxic stress, and were characterized by the increased intrachromosomal homologous recombination [Takeda et al, 2004]. Similarly, the expression of another gene, MIM1, involved in the maintenance of chromosome structure and required for the efficient homologous recombination, was significantly induced by DNA-damaging treatments [Hanin et al, 2000].…”

Section: Chromatin Remodeling Proteins: Shaping and Maintaining Chrommentioning

confidence: 99%

Epigenetic control of plant stress response

Boyko

Kovalchuk

2007

Environ and Mol Mutagen

308

194

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Living organisms have the clearly defined strategies of stress response. These strategies are predefined by a genetic make-up of the organism and depend on a complex regulatory network of molecular interactions. Although in most cases, the plant response to stress based on the mechanisms of tolerance, resistance, and avoidance has clearly defined metabolic pathways, the ability to acclimate/adapt after a single generation exposure previously observed in several studies (Boyko A et al. [2007]: Nucleic Acids Res 35:1714-1725; Boyko and Kovalchuk, unpublished data), represents an interesting phenomenon that cannot be explained by Mendelian genetics. The latest findings in the field of epigenetics and the process of a reversible control over gene expression and inheritance lead to believe that organisms, especially plants, may have a flexible short-term strategy of the response to stress. Indeed, the organisms that can modify gene expression reversibly have an advantage in evolutionary terms, since they can avoid unnecessary excessive rearrangements and population diversification. This review covers various epigenetic processes involved in plant stress response. We focus on the mechanisms of DNA methylation and histone modifications responsible for the protection of somatic cells and inheritance of stress memories. Environ. Mol. Mutagen. 49:61-72, 2008. V V C 2007 Wiley-Liss, Inc.

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Elevated levels of intrachromosomal homologous recombination in Arabidopsis overexpressing the MIM gene

Cited by 53 publications

References 40 publications

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

Involvement of the ArabidopsisSWI2/SNF2Chromatin Remodeling Gene Family in DNA Damage Response and Recombination

Epigenetic control of plant stress response

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