Elevated Levels of Tubulin Transcripts Accompany the GA&lt;sub&gt;3&lt;/sub&gt;-Induced Elongation of Oat Internode Segments

Mendu, N.; Silflow, Carolyn D.

doi:10.1093/oxfordjournals.pcp.a078533

Cited by 9 publications

(5 citation statements)

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“…As expected, six genes expression (two melon genes, four cucurbita genes) were significantly upregulated by exogenous NAA at the stage. Previous studies indicated that tubulin genes were involved in cell expansion and played an essential role in cell division and elongation [40][41]. The expression of five cucurbita tubulin genes was enhanced by exogenous NAA at the VB stage.…”

Section: Discussionmentioning

confidence: 88%

“…The results showed that six unigenes encoding expansion (MELO3C017181.2, MELO3C025095.2, CmoCh11G001730, CmoCh01G005150, CmoCh03G004350, CmoCh10G002140) were up-regulated at the VB stage. Tubulin is involved in cell expansion by guiding nascent cellulose microfibrils deposition during vascular development [40][41]. We found that the expression of five tubulin genes (CmoCh03G009120, CmoCh07G010840, CmoCh14G000910, CmoCh07G006190,CmoCh16G003240) were also elevated by exogenous NAA application at the VB stage, but CmoCh03G009120, CmoCh07G010840, CmoCh14G000910, and CmoCh07G006190, except CmoCh16G003240, were down-regulated at the CA stage.…”

Section: Exogenous Naa Application Activated Expression Of Genes Rela...mentioning

confidence: 87%

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Transcriptomic Analysis and Physiological Characteristics of Exogenous Naphthylacetic Acid Application to Regulate the Healing process of Oriental Melon Grafted onto Squash

Xu¹,

Wu²,

Guo³

et al. 2022

Preprint

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The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA3, and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.

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Section: Discussionmentioning

confidence: 88%

Section: Exogenous Naa Application Activated Expression Of Genes Rela...mentioning

confidence: 87%

Transcriptomic Analysis and Physiological Characteristics of Exogenous Naphthylacetic Acid Application to Regulate the Healing process of Oriental Melon Grafted onto Squash

Xu¹,

Wu²,

Guo³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The same trend can be seen in Figure 3; starting from the injection point, the promoting effect of exogenous GA on the internode length becomes stronger and reaches a peak at around the 25th internode. The GA 3 used in this study was shown to promote internode elongation in various crops, such as soybeans [95], oats [96], and corn [97]. The promotion of internode elongation by GA 3 varied with the distance; we believe that because the injected dose was not saturated relative to the demands of the rapid growth period, this led to large consumption of the exogenous GA 3 near the injection point.…”

Section: Effects Of the Exogenous Treatments On The Internode Lengthsmentioning

confidence: 84%

Plasticity in the Morphology of Growing Bamboo: A Bayesian Analysis of Exogenous Treatment Effects on Plant Height, Internode Length, and Internode Numbers

Bai

Cao³

et al. 2023

Plants

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Sucrose (Suc) and gibberellin (GA) can promote the elongation of certain internodes in bamboo. However, there is a lack of field studies to support these findings and no evidence concerning how Suc and GA promote the plant height of bamboo by regulating the internode elongation and number. We investigated the plant height, the length of each internode, and the total number of internodes of Moso bamboo (Phyllostachys edulis) under exogenous Suc, GA, and control group (CTRL) treatments in the field and analyzed how Suc and GA affected the height of Moso bamboo by promoting the internode length and number. The lengths of the 10th–50th internodes were significantly increased under the exogenous Suc and GA treatments, and the number of internodes was significantly increased by the exogenous Suc treatment. The increased effect of Suc and GA exogenous treatment on the proportion of longer internodes showed a weakening trend near the plant height of 15–16 m compared with the CTRL, suggesting that these exogenous treatments may be more effective in regions where bamboo growth is suboptimal. This study demonstrated that both the exogenous Suc and GA treatments could promote internode elongation of Moso bamboo in the field. The exogenous GA treatment had a stronger effect on internode elongation, and the exogenous Suc treatment had a stronger effect on increasing the internode numbers. The increase in plant height by the exogenous Suc and GA treatments was promoted by the co-elongation of most internodes or the increase in the proportion of longer internodes.

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“…The results showed that six unigenes encoding expansion (MELO3C017181.2, MELO3C025095.2, CmoCh11G001730, CmoCh01G005150, CmoCh03G004350, CmoCh10G002140) were up-regulated at the VB stage. Tubulin is involved in cell expansion by guiding nascent cellulose microfibrils deposition during vascular development ( Mendu & Silflow, 1993 ; Mo et al, 2018 ). We found that the expression of five tubulin genes (CmoCh03G009120, CmoCh07G010840, CmoCh14G000910, CmoCh07G006190,CmoCh16G003240) were also elevated by exogenous NAA application at the VB stage, but CmoCh03G009120, CmoCh07G010840, CmoCh14G000910, and CmoCh07G006190, except CmoCh16G003240, were down-regulated at the CA stage.…”

Section: Resultsmentioning

confidence: 99%

“…As expected, six genes expression (two melon genes, four cucurbita genes) were significantly up-regulated by exogenous NAA at the VB stage. Previous studies indicated that tubulin genes were involved in cell expansion and played an essential role in cell division and elongation ( Mendu & Silflow, 1993 ; Mo et al, 2018 ). The expression of five cucurbita tubulin genes was enhanced by exogenous NAA at the VB stage.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Guo

et al. 2022

PeerJ

View full text Add to dashboard Cite

The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA 3 , and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.

show abstract

Elevated Levels of Tubulin Transcripts Accompany the GA<sub>3</sub>-Induced Elongation of Oat Internode Segments

Cited by 9 publications

References 0 publications

Transcriptomic Analysis and Physiological Characteristics of Exogenous Naphthylacetic Acid Application to Regulate the Healing process of Oriental Melon Grafted onto Squash

Transcriptomic Analysis and Physiological Characteristics of Exogenous Naphthylacetic Acid Application to Regulate the Healing process of Oriental Melon Grafted onto Squash

Plasticity in the Morphology of Growing Bamboo: A Bayesian Analysis of Exogenous Treatment Effects on Plant Height, Internode Length, and Internode Numbers

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

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