Elevated &lt;i&gt;amh&lt;/i&gt; Gene Expression in the Brain of Male Tilapia &lt;i&gt;(Oreochromis niloticus) &lt;/i&gt;during Testis Differentiation

Poonlaphdecha, Srisupaph; Pepey, Elodie; Huang, Shenghui; Canonne, Marc; Soler, Lucile; Mortaji, Sepideh; Morand, Sergé; Pfennig, Frank; Mélard, Charles; Baroiller, Jean‐François; D'Cotta, Hélèna

doi:10.1159/000322579

Cited by 44 publications

(39 citation statements)

References 131 publications

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“…Supporting the functional importance of AMH signaling in the pituitary, AMHR2 gene expression has also been identified in the pituitary of nonmammalian species, i.e. the teleost Nile Tilapia [67, 68]. The present work reinforces the intriguing hypothesis that AMH contributes to the pituitary control of gonadal functions by demonstrating that gonadotrope AMH receptivity is regulated by the neuroendocrine hormone GnRH.…”

Section: Discussionsupporting

confidence: 82%

GnRH Transactivates Human AMH Receptor Gene via Egr1 and FOXO1 in Gonadotrope Cells

et al. 2018

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Background/Objectives: Anti-Müllerian hormone (AMH) signaling is critical for sexual differentiation and gonadal function. AMH receptor type 2 (AMHR2) is expressed in extragonadal sites such as brain, and pituitary and emerging evidence indicates that AMH biological action is much broader than initially thought. We recently reported that AMH signaling enhances follicle-stimulating hormone synthesis in pituitary gonadotrope cells. However, mechanisms regulating AMHR2 expression in these extragonadal sites remain to be explored. Method/Results: Here, we demonstrated in perifused murine LβT2 gonadotrope cells that Amhr2 expression is differentially regulated by GnRH pulse frequency with an induction under high GnRH pulsatility. Furthermore, we showed that GnRH transactivates the human AMHR2 promoter in LβT2 cells. Successive deletions of the promoter revealed the importance of a short proximal region (–53/–37 bp) containing an Egr1 binding site. Using site-directed mutagenesis of Egr1 motif and siRNA mediated-knockdown of Egr1, we demonstrated that Egr1 mediates basal and GnRH-dependent activity of the promoter, identifying Egr1 as a new transcription factor controlling hAMHR2 expression. We also showed that SF1 and β-catenin are required for basal promoter activity and demonstrated that both factors contribute to the GnRH stimulatory effect, independently of their respective binding sites. Furthermore, using a constitutively active mutant of FOXO1, we identified FOXO1 as a negative regulator of basal and GnRH-dependent AMHR2 expression in gonadotrope cells. Conclusions: This study identifies GnRH as a regulator of human AMHR2 expression, further highlighting the importance of AMH signaling in the regulation of gonadotrope function.

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Section: Discussionsupporting

confidence: 82%

GnRH Transactivates Human AMH Receptor Gene via Egr1 and FOXO1 in Gonadotrope Cells

et al. 2018

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“…Data: Oreochromis niloticus Ijiri et al 2008;Baroiller et al 2009a;Poonlaphdecha et al 2011Poonlaphdecha et al , 2013Tao et al 2013; http://www.uniprot.org/uniprot/ E9RGV7), Oryzias latipes Klüver et al 2005;Nakamoto et al 2007;Shibata et al 2010;Siegfried 2010;Okubo et al 2011;Nakamura et al 2012), Odontesthes hatcheri (Hattori et al 2013), Oncorhynchus mykiss (Baron et al 2005;Sawatari et al 2007;Gohin et al 2011;Vizziano-Cantonnet et al 2011), D. rerio (Chiang et al 2001;Guo et al 2005;Von Hofsten and Olsson 2005;Kallivretaki et al 2007;Siegfried 2010;Gautier et al 2011b;Nakamura et al 2012), Astatotilapia burtoni (Böhne et al 2013;A. Böhne and W. Salzburger, unpublished results).…”

Section: Resultsunclassified

Genetics of Sexual Development: An Evolutionary Playground for Fish

2014

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Teleost fishes are the most species-rich clade of vertebrates and feature an overwhelming diversity of sex-determining mechanisms, classically grouped into environmental and genetic systems. Here, we review the recent findings in the field of sex determination in fish. In the past few years, several new master regulators of sex determination and other factors involved in sexual development have been discovered in teleosts. These data point toward a greater genetic plasticity in generating the male and female sex than previously appreciated and implicate novel gene pathways in the initial regulation of the sexual fate. Overall, it seems that sex determination in fish does not resort to a single genetic cascade but is rather regulated along a continuum of environmental and heritable factors.

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“…In the gonochoristic tilapia, amh and amhr2 expression in the brain and pituitary appears to modulate FSH and LH release, and occurs prior to amh upregulation in the gonad [Poonlaphdecha et al, 2011]. This may suggest a potential role for Amh in regulating brain-gonad communication via the HPG axis.…”

Section: Amhmentioning

confidence: 96%

Bending Genders: The Biology of Natural Sex Change in Fish

Todd

Hui

Muncaster

et al. 2016

Sex Dev

119

126

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Sexual fate is no longer seen as an irreversible deterministic switch set during early embryonic development but as an ongoing battle for primacy between male and female developmental trajectories. That sexual fate is not final and must be actively maintained via continuous suppression of the opposing sexual network creates the potential for flexibility into adulthood. In many fishes, sexuality is not only extremely plastic, but sex change is a usual and adaptive part of the life cycle. Sequential hermaphrodites begin life as one sex, changing sometime later to the other, and include species capable of protandrous (male-to-female), protogynous (female-to-male), or serial (bidirectional) sex change. Natural sex change involves coordinated transformations across multiple biological systems, including behavioural, anatomical, neuroendocrine, and molecular axes. We here review the biological processes underlying this amazing transformation, focussing particularly on its molecular basis, which remains poorly understood, but where new genomic technologies are significantly advancing our understanding of how sex change is initiated and progressed at the molecular level. Knowledge of how a usually committed developmental process remains plastic in sequentially hermaphroditic fishes is relevant to understanding the evolution and functioning of sexual developmental systems in vertebrates generally, as well as pathologies of sexual development in humans.

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Elevated <i>amh</i> Gene Expression in the Brain of Male Tilapia <i>(Oreochromis niloticus) </i>during Testis Differentiation

Cited by 44 publications

References 131 publications

GnRH Transactivates Human AMH Receptor Gene via Egr1 and FOXO1 in Gonadotrope Cells

GnRH Transactivates Human AMH Receptor Gene via Egr1 and FOXO1 in Gonadotrope Cells

Genetics of Sexual Development: An Evolutionary Playground for Fish

Bending Genders: The Biology of Natural Sex Change in Fish

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