Elevated Metabolites of Steroidogenesis and Amino Acid Metabolism in Preadolescent Female Children With High Urinary Bisphenol A Levels: A High-Resolution Metabolomics Study

Abstract

Health risks associated with bisphenol A (BPA) exposure are controversially highlighted by numerous studies. High-resolution metabolomics (HRM) can confirm these proposed associations and may provide a mechanistic insight into the connections between BPA exposure and metabolic perturbations. This study was aimed to identify the changes in metabolomics profile due to BPA exposure in urine and serum samples collected from female and male children (n = 18) aged 7-9. Urine was measured for BPA concentration, and the children were subsequently classified into high and low BPA groups. HRM, coupled with Liquid chromatography-mass spectrometry/MS, followed by multivariate statistical analysis using MetaboAnalyst 3.0, were performed on urine to discriminate metabolic profiles between high and low BPA children as well as males and females, followed by further validation of our findings in serum samples obtained from same population. Metabolic pathway analysis showed that biosynthesis of steroid hormones and 7 other pathways-amino acid and nucleotide biosynthesis, phenylalanine metabolism, tryptophan metabolism, tyrosine metabolism, lysine degradation, pyruvate metabolism, and arginine biosynthesis-were affected in high BPA children. Elevated levels of metabolites associated with these pathways in urine and serum were mainly observed in female children, while these changes were negligible in male children. Our results suggest that the steroidogenesis pathway and amino acid metabolism are the main targets of perturbation by BPA in preadolescent girls.

Non-targeted metabolomics reveals alterations in liver and plasma of gilt-head bream exposed to oxybenzone

“…In fact, increased hippurate levels after oxybenzone administration (see Table S2) are not surprising since it was also observed with other xenobiotics such as toluene or arsenic (Amorim and Alvarez-Leite, 1997;Colet, 2015;Zhang et al, 2014), suggesting a non-specific mechanism of action (Robertson, 2005). In addition to hippurate, in the phenylalanine metabolism, we also observed the perturbations of trans-2,3,dihydroxycinnamate and phenylacetylglycine, and the later was also found as altered metabolite in children exposed to bisphenol A (Khan et al, 2017). Therefore, similarly to other…”

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