Elevated paraquat resistance can be used as a bioassay for longevity in a genetically based long‐lived strain of <i>Drosophila</i>

Arking, Robert; Buck, Steven; Berrios, Angela; Dwyer, Susan B.; Baker, George T.

doi:10.1002/dvg.1020120505

Cited by 134 publications

(65 citation statements)

References 23 publications

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“…), while the L-type animals raised under LD conditions have a survival rate of 5.0 per cent (± 5.0 per cent S.E.M.) (Arking et al, 1991). These differences are statistically significant.…”

Section: Methodsmentioning

confidence: 80%

“…Stock construction, measurement of life span, culture conditions, use of the paraquat test to test the presence or absence of the extended-longevity phenotype, and data analysis were carried out as described by Buck et al (1993) and Arking et a!. (1991).…”

Section: Methodsmentioning

confidence: 99%

“…The 5-day-old adults were assayed for paraquat sensitivity, known to be a test for longevity (Arking et a!., 1991) to determine the expression of the extended-longevity phenotype. Under standard conditions, the 5-day-old adult L type animals raised under HD conditions have a survival rate after 48 h exposure to 15 mM exogenous paraquat of 59.5 per cent ( 6.6 per cent S.E.M.…”

Section: Methodsmentioning

confidence: 99%

“…Animals known to display the ELP have an increased paraquat resistance, the two traits being inseparable by any of the several tests we have employed (Arking et a!., 1991). The data are shown in Fig.…”

Section: Statistical Analysis Of Longevitymentioning

confidence: 98%

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Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

et al. 1993

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Our previous work has shown that the major genes involved in the expression of the extendedlongevity phenotype are located on the third chromosome. Furthermore, their expression is negatively and positively influenced by chromosomes 2 and 1, respectively. In this report we show that the expression of the extended-longevity phenotype is dependent on the larval environment. A controlled chromosome substitution experiment was carried out using a strain selected for long life (L) and its parent (R) strain. Twenty different combinations of the three major chromosomes were conducted and their longevities were determined under both high (HD) and low (LD) larval density conditions. The extended-longevity phenotype was only expressed under RD conditions. The chromosome interactions were not apparent under LD conditions. Density-shift experiments delineate a critical period for expression of the extended-longevity phenotype, extending from 60 h after egg laying (AEL) to 96 h AEL, during which the developing animal must be exposed to HD conditions if the extended-longevity phenotype is to be expressed. The change from HD to LD conditions is accompanied by statistically significant increases in body weight. The possible role of a dietary restriction phenomenon is examined and the implications of these findings discussed. It is now apparent, however, that the extended-longevity phenotype in Drosophila is a developmental genetic process.

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“…), while the L-type animals raised under LD conditions have a survival rate of 5.0 per cent (± 5.0 per cent S.E.M.) (Arking et al, 1991). These differences are statistically significant.…”

Section: Methodsmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Statistical Analysis Of Longevitymentioning

confidence: 98%

See 2 more Smart Citations

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

et al. 1993

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“…As such, late-flowering mutants can also be described as extended longevity mutants. Studies with Drosophila have shown that paraquat tolerance can serve as a bio-marker for extended longevity (Arking et al, 1991). This also appears to be the case in Arabidopsis.…”

Section: Discussionmentioning

confidence: 87%

Oxidative stress tolerance and longevity in Arabidopsis: the late‐flowering mutant gigantea is tolerant to paraquat

et al. 1998

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SummaryRecent genetic analyses of longevity in animals have revealed that long-lived strains are more tolerant to environmental stresses. To investigate whether extended longevity in Arabidopsis also correlates with an increase in stress tolerance, the response was tested of 11 lateflowering mutants to the superoxide radical-generating herbicide paraquat. A tight correlation between flowering time and paraquat tolerance was found when plants were exposed to low doses of herbicide. Furthermore, the mutant gigantea (gi-3) with the longest delay in flowering time had a high tolerance level to paraquat-induced oxidative stress. All the tested gi alleles had an increased tolerance to paraquat toxicity compared to wild-type, although the actual levels of tolerance differed. In addition, the gi-3 mutant was more tolerant to hydrogen peroxide. These results suggest that the link between longevity and oxidative stress resistance in plants is similar to that found in animals, implying that this phenomenon may be general for all aerobic organisms.

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Aging mechanisms in fruit flies

Tower

1996

BioEssays

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Genetic analysis of Drosophila has provided evidence in support of two proposed evolutionary genetic mechanisms of aging: mutation accumulation and antagonistic pleiotropy. Both mechanisms result from the lack of natural selection acting on old organisms. Analyses of large numbers of files have revealed that mortality rates do not continue to rise with age as previously thought, but plateau at advanced ages. This phenomenon has implications both for models and for definitions of aging, and may be explained by the evolutionary theories. The physiological processes and genes most relevant to aging are being identified using Drosophila lines selected in the laboratory for postponed senescence. Oxidative stress and insufficient metabolic reserves/capacity may be particularly important factors in limiting the fruitfly lifespan. Genes which exhibit aging-related changes in expression are now being identified. Transgenic files are being used to analyze the mechanisms of such aging-related gene expression, and to test the effects of specific genes on aging and aging-related deterioration.

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Elevated paraquat resistance can be used as a bioassay for longevity in a genetically based long‐lived strain of Drosophila

Cited by 134 publications

References 23 publications

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

Oxidative stress tolerance and longevity in Arabidopsis: the late‐flowering mutant gigantea is tolerant to paraquat

Aging mechanisms in fruit flies

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