Elevated plasma levels of F_2α isoprostane in cystic fibrosis

Abstract

Cystic fibrosis (CF) is associated with chronic lung infection, inflammation, and elevated indices of oxidative stress. Recently, isoprostanes were shown to be a reliable in vivo marker of oxidant injury with 8-iso-PGF2 alpha, shown to cause airflow obstruction and plasma exudation in guinea pig lung. The present study was designed to examine the relationship between 8-iso-PGF2 alpha levels, plasma antioxidants, and clinical status in CF. We hypothesized that plasma 8-iso-PGF2 alpha levels would be higher in subjects with CF compared to healthy controls. Plasma 8-iso-PGF2 alpha levels were prospectively measured in 22 subjects with CF and nine healthy controls using an 8-isoprostane enzyme immunoassay kit along with plasma vitamins A, E, and beta-carotene. Plasma 8-iso-PGF2 alpha levels were shown to be significantly elevated in the CF subjects compared to controls (319.6 +/- 52.6 vs. 145.0 +/- 21.0 pg/mL, P = 0.005). Plasma levels of antioxidants were significantly lower for the CF subjects compared to the controls (vitamin A, P < 0.003; vitamin E, P < 0.001; and beta-carotene, P < 0.01). This study confirms significantly elevated lipid peroxidation in CF using 8-iso-PGF2 alpha levels.

Association between lipid peroxidation and inflammation in obstructive sleep apnoea

“…8-isoprostane is widely studied as a marker of oxidative stress in various respiratory diseases, including bronchial asthma, chronic obstructive pulmonary disease, cystic fibrosis and interstitial lung diseases [29][30][31][32]. Increased lipid peroxidation has also been reported in patients with OSA.…”

Plasma 8-Isoprostane Is Increased in Preterm Infants Who Develop Bronchopulmonary Dysplasia or Periventricular Leukomalacia

“…Elevated 8-isoprostane concentrations have been measured as a marker of in vivo oxidative stress during the first 2 d of life in tracheal aspirates of term infants with severe pulmonary disease (20), in bronchoalveolar lavage fluid in adults with interstitial lung diseases (23), in plasma in cystic fibrosis (24), and in exhaled breath condensate in adult respiratory distress syndrome (25). It has also been speculated that isoprostanes likely not only are markers of oxidative stress but also play a role in pulmonary pathophysiology, as they evoke important biologic responses in many cell types of the lung.…”

Association of Systemic Oxidative Stress with Suppressed Serum IgG to Commensal Oral Biofilm and Modulation by Periodontal Infection

“…Also, the mucosal tissue wounding associated with deep periodontal pockets has been associated with an increased occurrence of bacteremia (38). In this regard, 8-isoprostane concentrations have been demonstrated to be increased in Helicobacter pylori (H. pylori)-positive subjects (16), noncirrhotic chronic hepatitis C patients (30) and cystic fibrosis patients (15), as well as animal models of endotoxemia (3). Because the presence of periodontitis can result in recurring systemic exposure to oral biofilm microorganisms (38), it is conceivable that the resulting low-level bacteremia, endotoxemia, and consequent metabolic stress could contribute to an elevated serum concentration of serum 8-isoprostane.…”