Elevated proline content in maize plants expressing a fragment of the proline dehydrogenase gene in antisense orientation

Moiseeva, E. M.; Agafonov, D. A.; Velikov, V. А.; Volokhina, I. V.; Chumakov, M. I.

doi:10.1134/s1021443712020070

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Крім того цим рослинам була властива перехресна стійкість до водного дефіциту і сульфатно-хлоридного засолення [10]. Також показано підвищення рівня Pro в проростках трансгенних рослин кукурудзи при низьких рівнях хлоридного засолення [11]. Тотожна властивість була характерною і для генетично змінених рослин пшениці [6].…”

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Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene

Komisarenko,

Mykhalska

2023

Fakt. eksp. evol. org.

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Aim. To investigate the level of tolerance to water deficit of seed generations (T1 and T4) of genetically modified winter wheat with partially suppressed expression of the proline dehydrogenase gene (ProDH) based on the analysis of physiological and biochemical indicators and economic characteristics of plants. Methods. Determination of indicators of crop structure and content of free L-proline (Pro). Results. The level of Pro was studied and the main elements of productivity in the offspring of transgenic plants and their original forms under normal and insufficient water supply were analyzed. Conclusions. T1 and T4 biotechnological plants under normal/stress conditions accumulated more Pro than the original genotypes. The level of this amino acid in genetically modified seedlings under optimal cultivation conditions exceeded the initial forms by an average of 1.8 times. Under the influence of water deficit, its content increased by 2.2 and 2.3 times, in relation to normal water supply. In non-transgenic variants, the level of Pro, under similar growing conditions, was lower by 1.9 and 2.0 times, compared to T1 and T4 plants. Under the optimal water regime, the analyzed wheat variants did not differ significantly in terms of productivity. Drought led to their decrease, but the offspring of biotechnological plants were characterized by a less pronounced difference, as well as a higher yield.

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Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene

Komisarenko,

Mykhalska

2023

Fakt. eksp. evol. org.

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A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana

Lü

Yang

et al. 2017

Plant Physiology and Biochemistry

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Drought-tolerance of transgenic winter wheat with partial suppression of the proline dehydrogenase gene

Дубровна

Priadkina

Mykhalska

et al. 2022

Regul. Mech. Biosyst.

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The global climate changes and the consequent increase in the number of soil and air droughts during the vegetation period of grain crops require the development of new strategies to adapt plants to those yield-decreasing stressors. A relevant way of increasing drought-tolerance of cereals is the use of biotechnological methods, particularly RNA interference, which can down-regulate the activity of plants’ genes and increase concentration of stress metabolites that perform osmoprotective functions during drought. We studied the tolerance to soil moisture shortage in transgenic plants of winter wheat with partial suppression of the proline dehydrogenase gene, obtained using the technology of short interfering RNAs. We analyzed physiological and biochemical parameters and structural elements of yield productivity of 4 wild genotypes and their transgenic lines with reduced activity of proline dehydrogenase in the conditions of 7-day drought during the late booting–ear emergence. We determined that the presence of double-stranded RNA suppressor of the proline dehydrogenase gene in transgenic lines led to increase in the level of accumulation of free proline in flag leaves. At the same time, its concentration in transgenic lines was higher than in untransformed plants of the wild genotypes in both drought conditions and conditions of sufficient moisture. We found that against the background of water deficiency, the total chlorophyll content in leaves of plants of transgenic lines was significantly higher, and the ratio of carotenoids to chlorophyll was lower than in plants of the wild genotypes, suggesting mitigation of the negative impact of drought on the plants of transgenic lines. Lacking soil moisture, genetically altered lines of wheat had significantly higher parameters of the structure of grain yield compared with untransformed genotypes. At the same time, we observed genotypic difference according to grain productivity in biotechnological plants. Therefore, the results we obtained confirm the perspectives of using the technology of short interfering RNAs to increase tolerance of winter wheat to water deficiency.

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Elevated proline content in maize plants expressing a fragment of the proline dehydrogenase gene in antisense orientation

Cited by 3 publications

References 9 publications

Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene

Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene

A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana

Drought-tolerance of transgenic winter wheat with partial suppression of the proline dehydrogenase gene

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