Elevated ROS-scavenging enzymes contribute to acclimation to UV-B exposure in transplastomic tobacco plants, reducing the role of plastid peroxidases

Czégény, Gyula; Martret, Bénédicte Le; Pávkovics, Dóra; Dix, Philip J.; Hideg, Éva

doi:10.1016/j.jplph.2016.05.026

Cited by 25 publications

(12 citation statements)

References 31 publications

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“…The antioxidant marker gene GPX is upregulated under abiotic stress and is thought to be involved in ROS scavenging in Panax ginseng 63 . GSR activity was induced by oxidative stress caused by UV-B radiation in transgenic tobacco plants, thereby giving the plants increased resistance to UV-B stress 64 – 66 . The increased expression of unigenes encoding GPX and GSR indicates that ROS or other free radicals might be generated in G .…”

Section: Discussionmentioning

confidence: 99%

Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation

Zhang

Ding

et al. 2018

Sci Rep

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Plants respond to UV-B irradiation (280–315 nm wavelength) via elaborate metabolic regulatory mechanisms that help them adapt to this stress. To investigate the metabolic response of the medicinal herb Chinese liquorice (Glycyrrhiza uralensis) to UV-B irradiation, we performed liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomic analysis, combined with analysis of differentially expressed genes in the leaves of plants exposed to UV-B irradiation at various time points. Fifty-four metabolites, primarily amino acids and flavonoids, exhibited changes in levels after the UV-B treatment. The amino acid metabolism was altered by UV-B irradiation: the Asp family pathway was activated and closely correlated to Glu. Some amino acids appeared to be converted into antioxidants such as γ-aminobutyric acid and glutathione. Hierarchical clustering analysis revealed that various flavonoids with characteristic groups were induced by UV-B. In particular, the levels of some ortho-dihydroxylated B-ring flavonoids, which might function as scavengers of reactive oxygen species, increased in response to UV-B treatment. In general, unigenes encoding key enzymes involved in amino acid metabolism and flavonoid biosynthesis were upregulated by UV-B irradiation. These findings lay the foundation for further analysis of the mechanism underlying the response of G. uralensis to UV-B irradiation.

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Section: Discussionmentioning

confidence: 99%

Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation

Zhang

Ding

et al. 2018

Sci Rep

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“…Such mobility facilitates the molecule's messenger function but also requires the antioxidant control of local concentrations further away from H 2 O 2 production sites. Ultraviolet-exposed plants especially need to maintain an effective H 2 O 2 regulating system [17][18][19][20][21][22] since the UV-B (280-315 nm) component of sunlight not only elevates H 2 O 2 concentrations in planta but may also photoconvert H 2 O 2 to more hazardous hydroxyl radicals ( • OH) 8 . Earlier studies have shown that the proper activation of class-III plant peroxidase (POD) enzymes is a key factor in the successful acclimation to UV-B both in model plants exposed to supplemental UV radiation in growth chambers 20 and in sun leaves outdoors 23 .…”

Section: Y(no)mentioning

confidence: 99%

Ultraviolet-B acclimation is supported by functionally heterogeneous phenolic peroxidases

Rácz

Czégény

Csepregi

et al. 2020

Sci Rep

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Tobacco plants were grown in plant chambers for four weeks, then exposed to one of the following treatments for 4 days: (1) daily supplementary UV-B radiation corresponding to 6.9 kJ m−2 d−1 biologically effective dose (UV-B), (2) daily irrigation with 0.1 mM hydrogen peroxide, or (3) a parallel application of the two treatments (UV-B + H2O2). Neither the H2O2 nor the UV-B treatments were found to be damaging to leaf photosynthesis. Both single factor treatments increased leaf H2O2 contents but had distinct effects on various H2O2 neutralising mechanisms. Non-enzymatic H2O2 antioxidant capacities were increased by direct H2O2 treatment only, but not by UV-B. In contrast, enzymatic H2O2 neutralisation was mostly increased by UV-B, the responses showing an interesting diversity. When class-III peroxidase (POD) activity was assayed using an artificial substrate (ABTS, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), both treatments appeared to have a positive effect. However, only UV-B-treated leaves showed higher POD activities when phenolic compounds naturally occurring in tobacco leaves (chlorogenic acid or quercetin) were used as substrates. These results demonstrate a substrate-dependent, functional heterogeneity in POD and further suggest that the selective activation of specific isoforms in UV-B acclimated leaves is not triggered by excess H2O2 in these leaves.

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“…The quenching capacity of carotenoids towards different ROS has been widely described (Fiedor & Burda, 2014), as well as their modulation under UV-B radiation (Liu et al, 2011;Castagna et al, 2013). Since UV-B radiation is a potential source of oxidative stress (Czégény, Le Martret, Pávkovics, Dix, & Hideg, 2016), it might be possible that carotenoids were consumed to counteract the potentially damaging ROS. This possibility is in line with the results observed for the phenolic compounds.…”

Section: Terpenoidsmentioning

confidence: 99%

Post-harvest UV-B radiation modulates metabolite profile in peach fruit

Santin

Lucini

Castagna

et al. 2018

Postharvest Biology and Technology

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The possibility to modify plant metabolic profile of plants and fruit to improve their healthy properties using eco-friendly tools, rather than transgenic approaches, gained interest in the last decades. Ultraviolet-B (UV-B) radiation, at low levels, thanks to its ability to influence plant secondary metabolism, could be successfully used to achieve this goal. However, few studies have been conducted so far on the effects of post-harvest UV-B treatments on fruit metabolomics. The present research, aimed to evaluate the impact of UV-B on peach metabolites profile through nontargeted metabolomics (UHPLC-ESI/QTOF-MS) coupled with multivariate chemometrics, provided evidence that 10 and 60 min of post-harvest UV-B irradiation influenced several classes of metabolites. Most phenolics were downaccumulated 24 h after both UV-B treatments, though, after 36 h, anthocyanins, flavones and dihydroflavonols increased (2.06-, 1.92-, 1.68-fold with 10 min UV-B; 6.65-, 2.53-, 2.05-fold with 60 min UV-B, respectively). UV-B reduced carotenoids and most lipids and increased some biosynthetic intermediates and degradation products, some of them known for their positive role in human health. Among alkaloids, some pteridines accumulated, likely derived from folates degradation, while indole alkaloids decreased. Despite the decrease of some bioprotective metabolites as carotenoids, the UV-B-induced up-accumulation of many antioxidant phenolics after 36 h from the exposure suggests an improvement of the healthy properties of peach fruit and reinforces the potential of UV-B controlled irradiation as a nutraceuticals-increasing tool in fruit.

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Elevated ROS-scavenging enzymes contribute to acclimation to UV-B exposure in transplastomic tobacco plants, reducing the role of plastid peroxidases

Cited by 25 publications

References 31 publications

Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation

Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation

Ultraviolet-B acclimation is supported by functionally heterogeneous phenolic peroxidases

Post-harvest UV-B radiation modulates metabolite profile in peach fruit

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