Elevated sister chromatid exchange frequencies in the lymphocytes of esophageal cancer patients

Adhvaryu, Siddharth G.; Rawal, Urwashi M.; Patel, J. D.

doi:10.1002/1097-0142(19880501)61:9<1867::aid-cncr2820610924>3.0.co;2-f

Cited by 14 publications

(2 citation statements)

References 29 publications

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“…For the reasons pointed out P C above, these observations also cannot be judged to absolutely disagree with our findings, as the majority of patients with rectal cancer, indeed, was not burdened with a hbloodlympho-increased chromosomal instability or sensitivity to -eakage rates for clastogens. Reports on increased SCE frequencies in 'perimental pro-blood lymphocytes of cancer patients (29)(30)(31)(32)(33), however, could not be confirmed by the present investigation. and the neoplasms were carcinomas in most cases, the use of inappropriate and therefore uninformative cells might also explain some of these discrepancies.…”

Section: Discussionmentioning

confidence: 65%

Cytogenetic studies in lymphocytes of patients with rectal cancer.

Gebhart

Romahn

Schneider

et al. 1993

Environ Health Perspect

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Spontaneous and clastogen-induced chromosomal instability in a high-risk group (i.e., 33 patients with rectal carcinomas) was investigated using peripheral blood lymphocytes as target cells. In addition to the analysis of spontaneous and clastogen-induced chromosome aberrations, this study also included classical karyotype analysis and scoring of sister chromatid exchanges (SCE) in some of the patients. Diepoxybutane (DEB), 4-nitroquinoline-1-oxide (NQO), and bleomycin were used as standard clastogens. Lymphocytes of healthy control individuals were studied in parallel with each cancer patient. While only slight but significant differences could be detected of the average spontaneous, DEB-and bleomycin (G2)-induced chromosome breakage between patient and control lymphocytes, individual patients and two of the control individuals showed a more distinct increase in the frequency of the studied end points. These increases were documented by a variegated mosaicism of karyotypic changes and by an increased breakage rate induced by the clastogens.Neither the bleomycin-exposure in the G, phase nor SCE was capable of detecting differences between the patients and controls. Of particular interest in the sense of high-risk individuals were seven patients and two control persons whose lymphocytes exhibited increased chromosomal sensitivity under more than one of the studied experimental conditions.

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Section: Discussionmentioning

confidence: 65%

Cytogenetic studies in lymphocytes of patients with rectal cancer.

Gebhart

Romahn

Schneider

et al. 1993

Environ Health Perspect

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“…Similar results showing no differences between cancer cases and healthy controls were published before [ 35 – 37 ]. However, it is worth mentioning that study results are not consistent [ 38 , 39 ] in this area.…”

Section: Discussionmentioning

confidence: 85%

Can consumption of raw vegetables decrease the count of sister chromatid exchange? Results from a cross-sectional study in Krakow, Poland

Gałaś

Cebulska-Wasilewska

2014

Eur J Nutr

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Background Sister chromatid exchange (SCE) is a widely used sensitive cytogenetic biomarker of exposure to genotoxic and cancerogenic agents. Results of human monitoring studies and cytogenetic damage have revealed that biological effects of genotoxic exposures are influenced by confounding factors related to life-style. Vegetable and fruit consumption may play a role, but available results are not consistent. The purpose of the study was to investigate the effect of consumption of raw and cooked vegetables and fruits on SCE frequency.MethodsA total of 62 participants included colorectal cancer (CRC) patients, hospital-based controls and healthy laboratory workers. SCE frequency was assessed in blood lymphocytes. Frequency of vegetable and fruit consumption was gathered by structured semi-quantitative food frequency questionnaire.ResultsSCE frequency was lowest among hospital-based controls (4.4 ± 1.1), a bit higher in CRC patients (4.5 ± 1.0) and highest among laboratory workers (7.4 ± 1.2) (p < 0.05). Multivariable linear regression showed a significant inverse effect (b = −0.20) of raw vegetable consumption, but not so for intake of cooked vegetables and fruits.ConclusionsThe results of the study have shown the beneficial effect of consumption of raw vegetables on disrupted replication of DNA measured by SCE frequency, implying protection against genotoxic agents. Further effort is required to verify the role of cooked vegetables and fruits.

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Sister chromatid exchange frequencies in carcinoma of the human oral cavity: Effect of treatment

et al. 1992

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Spontaneous and mitomycin-C-induced sister chromatid exchange frequencies were studied in the lymphocytes of 25 oral cancer patients. For these patients, the mean spontaneous SCE value was 8.31 (+/- SD), which was significantly higher than the value of 6.60 (+/- SD) obtained for the controls (p < 0.001). But there was no significant difference between the Mitomycin C induced mean SCE values of oral cancer patients and controls. Seven of these 25 oral cancer patients were selected for second sampling after surgical removal of the tumor. Six of these seven patients showed a decrease in mean SCE/cell value following surgery, which was almost equal to the SCE values obtained for the controls. This indicates that the increased spontaneous SCE rates in oral cancer patients might be due to the metabolic stress imposed by the tumor on the host body or by some clastogenic product of the malignant cells. Surgical removal of the tumour might have resulted in normalization of the lymphocytic SCE rates in postoperatively studied patients.

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Elevated sister chromatid exchange frequencies in the lymphocytes of esophageal cancer patients

Cited by 14 publications

References 29 publications

Cytogenetic studies in lymphocytes of patients with rectal cancer.

Cytogenetic studies in lymphocytes of patients with rectal cancer.

Can consumption of raw vegetables decrease the count of sister chromatid exchange? Results from a cross-sectional study in Krakow, Poland

Sister chromatid exchange frequencies in carcinoma of the human oral cavity: Effect of treatment

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