Elevated sperm chromosome aneuploidy and apoptosis in patients with unexplained recurrent pregnancy loss

Abstract

These data indicate that some recurrent pregnancy loss patients have a significant increase of sperm chromosome aneuploidy, apoptosis, and abnormal sperm morphology. This study demonstrates a new possible cause of recurrent pregnancy loss.

Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization/intracytoplasmic sperm injection: a systematic review and meta-analysis

“…Indeed, an association between sperm DNA damage and pregnancy/ miscarriage has been observed in non-IVF studies. A study by Carrell et al (29) reported that recurrent miscarriage is related to higher levels of sperm DNA damage, and Evenson et al (46) observed that pregnancy loss rate increased significantly with sperm DNA damage. Animal studies (47)(48)(49) regarding the possible mechanism underling the association between sperm DNA damage and miscarriage showed that sperm DNA damage can lead to abnormal embryo development and impaired embryo implantation.…”

“…Some studies indicated that sperm with DNA damage can fertilize oocytes successfully and give rise to good-grade embryos that subsequently result in early pregnancy loss (24,(26)(27)(28)(29)(30)(31).…”

Use of automated imaging and analysis technology for the detection of aneuploidy in human sperm

“…The technician scored 10 donor sperm samples using standard scoring criteria (1,9) and an upright microscope equipped with epifluorescence and the appropriate filter set for AneuVysion probes (Vysis) at Â1,000 magnification.…”

Localization of single-stranded DNA in human sperm nuclei

“…Infertile couples in whom the man has high levels of sperm DNA damage experience lower pregnancy rates and a higher rate of spontaneous abortions (6,28,29). Couples undergoing insemination also tend to have poorer pregnancy outcome when the man's spermatozoa possess high levels of DNA damage (4,5).…”