Elevated urinary d-lactate levels in patients with diabetes and microalbuminuria

Chou, Chia-Hung; Lee, Ya Ting; Chen, Shih Ming; Hsieh, Chi Wen; Huang, Tzu Chuan; Li, Yi Chieh; Lee, Jen Ai

doi:10.1016/j.jpba.2015.06.014

Cited by 33 publications

(27 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to the traditional diabetic marker hemoglobin A1C (HbA1 C ), skin AGEs such as MG-H1 correlate better with diabetic nephropathy parameters. Another study shows that urine d-lactate, a downstream metabolite of MGO, is higher in DMN subjects than healthy subjects [35][36][37]. These clinical data suggest MGO is crucial for progression of diabetic nephropathy [4,38].…”

Section: Methylglyoxal Generation Metabolism and Damagementioning

confidence: 93%

“…Even pre-microalbuminuria phase diabetic patients (albumin-to-creatinine ratio, ACR, less than 30 mg albumin/mg creatinine) have elevated urine d-lactate levels compared with normal control. As the ACR worsens to 30-299 (micro-albuminuria), the urine d-lactate level increases [37]. In AAN mice models, urine d-lactate is also higher, and can reflect the renal injury [48].…”

Section: Methylglyoxal and Nephropathy In Diabetic And Non-diabetic Mmentioning

confidence: 99%

“…D-lactate is tested for bowel ischemia, a special type of lactate acidosis; this molecule may be a prognostic predictor for sepsis [58][59][60][61][62]. Urine d-lactate, the metabolite of MGO excreted in urine, may proxy for MGO and carbonyl stress, and it may be considered an early marker of diabetic injury [37]. The analytical methods used in the determination of d-lactate use HPLC [37] and liquid chromatography tandem mass spectrometry [73].…”

Section: Methylglyoxal Usage In Clinical Settingsmentioning

confidence: 99%

“…Urine d-lactate, the metabolite of MGO excreted in urine, may proxy for MGO and carbonyl stress, and it may be considered an early marker of diabetic injury [37]. The analytical methods used in the determination of d-lactate use HPLC [37] and liquid chromatography tandem mass spectrometry [73]. However, a newly developed d-lactate assay system, using d-lactate dehydrogenase to catalyze d-lactate and detect the catalysis with a UV-light-emitting diode, was reported recently [74].…”

Section: Methylglyoxal Usage In Clinical Settingsmentioning

confidence: 99%

See 3 more Smart Citations

Evolving Evidence of Methylglyoxal and Dicarbonyl Stress Related Diseases from Diabetic to Non-Diabetic Models

Wang¹,

Lee²,

Chou³

2015

Pharm Anal Acta

View full text Add to dashboard Cite

Methylglyoxal (MGO), a byproduct of sugar and lipid metabolic processes, is a major glycating agent. This metabolite reacts with basic residues of proteins and promotes the formation of advanced glycation end products (AGEs). Although MGO and AGEs are widely discussed in the context of diabetes, until recently, MGO was thought to result from insufficient blood sugar control. A new report reveals that plasma MGO, and not blood sugar, distinguishes diabetic patients with no pain from those with pain. This ability brings to MGO a new applicability to disease diagnosis.Diseases with normal sugar conditions, such as hypertension, sepsis, and renal disease, are increasingly recognized as MGO-related. We review the role of MGO in drug-induced nephropathy, induction of hypertension by oral administration, and as a biomarker of sepsis. We also discuss the measurement of MGO and its stable metabolite d-lactate.The metabolism and pathogenic mechanisms of MGO need investigation in diverse disease models. Whether MGO can be considered as an individual pathological factor will be an interesting topic.

show abstract

Section: Methylglyoxal Generation Metabolism and Damagementioning

confidence: 93%

Section: Methylglyoxal and Nephropathy In Diabetic And Non-diabetic Mmentioning

confidence: 99%

Section: Methylglyoxal Usage In Clinical Settingsmentioning

confidence: 99%

Section: Methylglyoxal Usage In Clinical Settingsmentioning

confidence: 99%

See 2 more Smart Citations

Evolving Evidence of Methylglyoxal and Dicarbonyl Stress Related Diseases from Diabetic to Non-Diabetic Models

Wang¹,

Lee²,

Chou³

2015

Pharm Anal Acta

View full text Add to dashboard Cite

show abstract

“…Both LA and 2HG are α-hydroxy acids (AHAs). A variety of analytical methods for the separation and determination of the enantiomers of LA, 2HG, and other AHAs have been reported, including HPLC [7,15,16], HPLC-MS [17][18][19], CE [20,21], GC [22,23], and GC-MS [24], with a chiral or achiral stationary phase. GC-MS is considered to be superior to other techniques due to its high sensitivity and robustness.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of the enantiomers of lactic acid and 2‐hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry

Ding

Lin

Weng

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

Lactic acid and 2-hydroxyglutaric acid are chiral metabolites that have two distinct d- and l-enantiomers with distinct biochemical properties. Perturbations of a single enantiomeric form have been found to be closely related to certain diseases. Therefore, the ability to differentiate the d and l enantiomers is important for these disease studies. Herein, we describe a method for the separation and determination of lactic acid and 2-hydroxyglutaric acid enantiomers by chiral derivatization (with l-menthol and acetyl chloride) combined with gas chromatography and mass spectrometry. The two pairs of above-mentioned enantiomers exhibited linear calibration curves with a correlation coefficient (R ) exceeding 0.99. The measured data were accurate in the acceptable recovery range of 88.17-102.30% with inter- and intraday precisions (relative standard deviations) in the range of 4.23-17.26%. The limits of detection for d-lactic acid, l-lactic acid, d-2-hydroxyglutaric acid, and l-2-hydroxyglutaric acid were 0.13, 0.11, 1.12, and 1.16 μM, respectively. This method was successfully applied to analyze mouse plasma. The d-lactic acid levels in type 2 diabetes mellitus mouse plasma were observed to be significantly higher (P < 0.05, t-test) than those of normal mice, suggesting that d-lactic acid may serve as an indicator for type 2 diabetes mellitus.

show abstract

Real‐Time Lactate Detection in A Dynamic Environment Using Micrsensing Needles

Tang,

Huang,

Yang

et al. 2024

Advanced Sensor Research

View full text Add to dashboard Cite

This study introduces a novel microneedle‐based lactate sensor with SU‐8 micropillar enhancement, designed for real‐time monitoring in dynamic environments. Utilizing inkjet‐printing technology, the sensor demonstrates enhanced sensitivity and a reduced limit of detection (LoD), addressing critical challenges in clinical applications like hemodialysis and patient monitoring in ICU. Design enhancements in the medical steel needle improve stress resistance during insertion, contributing to the sensor's reliability. The experimental findings demonstrate that the microneedle is capable of achieving a high level of linearity at 0.99, with a sensitivity of 3.38 µA mM−1/mm−2–0.5 µA mM−1/mm−2 observed within the range of 0.1–0.5 mM and 1–10 mM, respectively. Meanwhile, the microneedle exhibits a low limit of detection (LoD) of 0.01 mM when tested in phosphate‐buffered saline (PBS) with varying lactate concentrations. Moreover, it demonstrates a linearity of 0.98, sensitivity of 1.13 µA mM−1 mm−2, and the same LoD of 0.01 mM in urine. The sensor maintains its performance at flow rates up to 500 mL min−1. Overall, this flexible and inkjet‐printed lactate sensor represents a significant advancement in real‐time clinical monitoring technology.

show abstract

Elevated urinary d-lactate levels in patients with diabetes and microalbuminuria

Cited by 33 publications

References 37 publications

Evolving Evidence of Methylglyoxal and Dicarbonyl Stress Related Diseases from Diabetic to Non-Diabetic Models

Evolving Evidence of Methylglyoxal and Dicarbonyl Stress Related Diseases from Diabetic to Non-Diabetic Models

Separation and determination of the enantiomers of lactic acid and 2‐hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry

Real‐Time Lactate Detection in A Dynamic Environment Using Micrsensing Needles

Contact Info

Product

Resources

About