Elevated urinary levels of carcinogenic N- nitrosamines in patients with urinary tract infections measured by isotope dilution online SPE LC–MS/MS

Hu, Chiung‐Wen; Shih, Ying-Ming; Liu, Hung-Hsin; Chiang, Yi-Chen; Chen, Chih‐Ming; Chao, Mu‐Rong

doi:10.1016/j.jhazmat.2016.02.048

Cited by 18 publications

(5 citation statements)

References 41 publications

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“…The method LODs were 0.02–0.1 μg L −1 , these values were lower than those obtained by a similar technique, 24 and even could be comparable with most LODs obtained by GC-MS or LC-MS/MS with an enrichment pretreatment procedure (Table 2). 21,25–30 In addition, high recoveries were obtained ranging from 79.4% to 108.0%, and the precision indicated by relative standard deviations (RSDs) were in the range of 2.3%–12.9% (Table 3). These results demonstrated that the recoveries and precision of the methods were in accordance with the criteria for quality control of laboratories for chemical testing.…”

Section: Resultsmentioning

confidence: 99%

Rapid and selective determination of 9 nitrosamines in biological samples using ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry

Zhang

Huang

et al. 2022

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

Rapid and selective determination of 9 nitrosamines in biological samples using ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry

Zhang

Huang

et al. 2022

Anal. Methods

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“…For this, Het‐1A cells were pretreated with 5 mM NAC (ROS scavenger) and 50 μM mito‐TEMPO (mtROS scavenger) for 30 min before exposure to nitrosamines, respectively. Our treated dose of 500 ng/mL was 10,000 times the nitrosamines concentration in human drinking water in China and equivalent to 25 times the nitrosamine concentration in human urine (Bei et al, 2016; Hu et al, 2016; Zhao et al, 2020). The fluorescent probes were used to detect the levels of ROS and mtROS and measure the changes in mitochondrial membrane potential.…”

Section: Resultsmentioning

confidence: 99%

“…(2) 20 ng/mL was close to the concentration of nitrosamines in human urine (Hu et al, 2016;Zhao, Lu, et al, 2019); (3) the maximum dose (2500 ng/mL) was lower than the genotoxic concentration (8 μg/mL) (Wagner et al, 2012).…”

Section: Cell Culture and Treatmentmentioning

confidence: 90%

“…The exposure dose setting in this study followed several criteria: (1) The dose range covered the exposure levels of nitrosamines that humans may be exposed to in the environment, and the nitrosamines concentration increased along with the certain gradient; (2) 20 ng/mL was close to the concentration of nitrosamines in human urine (Hu et al, 2016; Zhao, Lu, et al, 2019); (3) the maximum dose (2500 ng/mL) was lower than the genotoxic concentration (8 μg/mL) (Wagner et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

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Reactive oxygen species‐mediated activation of NLRP3 inflammasome associated with pyroptosis in Het‐1A cells induced by the co‐exposure of nitrosamines

Liu

Zhao

Zhou

et al. 2022

J of Applied Toxicology

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Nitrosamines were a class of important environmental carcinogens associated with digestive tract neoplasms. As the early toxic effect of nitrosamines, inflammatory response participated in the malignant transformation of cells and promoted the occurrence and development of tumors. However, the role of NLRP3 inflammasome in the nitrosamines-induced inflammatory response was unclear. In this study, the human esophageal epithelial cells (Het-1A) were used to explore potential mechanisms of the activation of NLRP3 inflammasome under co-exposure to nine nitrosamines commonly found in drinking water at the doses of 0, 4, 20, 100, 500, and 2500 ng/mL. The results showed that nitrosamines stimulated activation of the NLRP3 inflammasome and induced cellular oxidative damage in a dose-dependent manner. Pretreatment of reactive oxygen species scavenger N-acetyl-L-cysteine (NAC), particularly mitochondrial reactive oxygen species (mtROS) scavengers Mito-TEMPO, effectively inhibited the activation of NLRP3 inflammasome, suggesting that nitrosamines could mediate the activation of NLRP3 inflammasome via mtROS. Furthermore, we found that nitrosamines co-exposure also promoted cell pyroptosis through the NLRP3/caspase-1/GSDMD pathway, which was demonstrated by adding the caspase-1 inhibitor Z-YVAD-FMK and constructing NLRP3 downregulated Het-1A cell line. This study revealed the underlying mechanism of the activation of NLRP3 inflammasome initiated by nitrosamines co-exposure and provided new perspectives on the toxic effects of nitrosamines.

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“…There is no uniform standard method, which can quickly and accurately determine ng/L concentration NDMA. The existing methods can only rely on new instruments or related accessories to achieve the accuracy of detection [133][134][135][136][137]. The operation is complicated and not cost-effective.…”

Section: Monitoring Methodsmentioning

confidence: 99%

Occurrence and control of N-nitrosodimethylamine in water engineering systems

Bian

Wang

Zhu

et al. 2018

Environmental Engineering Research

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N-nitrosodimethylamine (NDMA) is a typical nitrogen disinfection by-product, which has posed a potential threat to human health during drinking water disinfection. Because of the well-known effects of mutagenesis, carcinogenesis and teratogenesis, the high detection rate in water engineering systems (such as coagulation, membrane filtration and biological systems), and difficulty to remove, it has received wide concern in the field of water engineering systems. The NDMA is a low molecular weight hydrophilic organic substance, which is difficult to remove. Also, the mechanism for NDMA formation is also recognized to be complex, and many steps still needed to be further evaluated. Therefore, the mechanistic knowledge on NDMA formation potential and their removal processes is of particularly interest. Few papers summarize the occurrence and control of NDMA in water engineering systems. It is for this reason that the content of this paper is particularly important for us to understand and control the amount of NDMA thus reducing the threat of disinfection by-products to drinking water. Four parts including the mechanisms for the NDMA formation potential, the factors affecting the NDMA formation potential, the technologies for removal of NDMA are summarized. Finally, some definite suggestions are given.

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Elevated urinary levels of carcinogenic N- nitrosamines in patients with urinary tract infections measured by isotope dilution online SPE LC–MS/MS

Cited by 18 publications

References 41 publications

Rapid and selective determination of 9 nitrosamines in biological samples using ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry

Rapid and selective determination of 9 nitrosamines in biological samples using ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry

Reactive oxygen species‐mediated activation of NLRP3 inflammasome associated with pyroptosis in Het‐1A cells induced by the co‐exposure of nitrosamines

Occurrence and control of N-nitrosodimethylamine in water engineering systems

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