Elevation of Glucose 6-Phosphate Dehydrogenase Activity Induced by Amplified Insulin Response in Low Glutathione Levels in Rat Liver

Taniguchi, Misako; Mori, Nobuko; Iramina, Chizuru; Yasutake, Akira

doi:10.1155/2016/6382467

Cited by 8 publications

(5 citation statements)

References 35 publications

(43 reference statements)

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“…An increase in GSH-Px activity is supported by the upregulation of GSH levels. In the cross-analysis reported in the present study, we noticed that the protein level of G6PD did not increase significantly, suggesting that the discrepancy between activity and protein levels was attributable to the oxidation/reduction states of G6PD (Luzzatto 1967;Taniguchi et al 2016), the glycosylation states of G6PD (Rao et al 2015), the post-translational deacetylated state (Wang et al 2014), and the stimulation caused by the phosphorylation of G6PD (Matte et al 2020).…”

Section: Discussioncontrasting

confidence: 42%

Enzymatic Changes in Red Blood Cells of Diamond-Blackfan Anemia

Utsugisawa

Uchiyama

Toki

et al. 2021

Tohoku J. Exp. Med.

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Diamond-Blackfan anemia is a congenital bone marrow failure syndrome characterized by red blood cell (RBC) aplasia with varied malformations in infants. Elevated activity of adenosine deaminase (ADA) has been considered as a useful biomarker of Diamond-Blackfan anemia, and ADA assay has been shown to be more sensitive than genetic diagnosis. Approximately, 80% of the examined patients showed elevated ADA activity, whereas genetic tests of ribosome subunit genes identified mutations in approximately 60% of the patients. We previously reported that reduced glutathione (GSH) levels in RBCs may serve as a biomarker of Diamond-Blackfan anemia. In this study, to confirm the universality of our data, we extended the analysis to seven RBC enzymes and GSH of 14 patients with Diamond-Blackfan anemia and performed a cross-analysis study using enzyme activity assay and recently reported proteome data. Statistical analysis revealed that both data exhibited high similarity, upregulation in the hexokinase and pentosephosphate pathway, and downregulation in glycolytic enzymes such as phosphofructokinase and pyruvate kinase, in the RBCs obtained from the subjects with Diamond-Blackfan anemia. The only discrepancy between enzyme activity and proteome data was observed in glucose-6-phosphate dehydrogenase (G6PD), as increased G6PD activity showed no relation with the significant elevation in protein levels. These results suggest that our enzymatic activity data of Diamond-Blackfan anemia are universal and that the enzymatic activation of G6PD via a hitherto-unveiled mechanism is another metabolic feature of RBCs of Diamond-Blackfan anemia.

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Section: Discussioncontrasting

confidence: 42%

Enzymatic Changes in Red Blood Cells of Diamond-Blackfan Anemia

Utsugisawa

Uchiyama

Toki

et al. 2021

Tohoku J. Exp. Med.

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“…This patient showed ~3 fold decrease in G6PD activity (28% of the control group mean value). According to a recent study by Taniguchi et al G6PD activity is correlated with the mRNA level [ 23 ]. We believe that it is a plausible explanation for an observed decrease in G6PD activity.…”

Section: Discussionmentioning

confidence: 99%

New cases of Glucose-6-Phosphate Dehydrogenase deficiency in Pulmonary Arterial Hypertension

et al. 2018

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Pulmonary Arterial Hypertension (PAH) is a fatal disorder with limited treatment options and reduced life expectancy after diagnosis. Complex genetic backgrounds in PAH complicates identification of causative mutations that is essential for an understanding of the disease diagnostics and etiology especially for idiopathic PAH (iPAH). Hemolysis has been implicated as contributing to the pathobiology of PAH. Glucose-6-Phosphate Dehydrogenase (G6PD) expression and activity define erythrocyte’s antioxidant capacity, and its decrease contributes to erythrocyte fragility. As G6PD deficiency was previously reported in a limited number of PAH cases, we tested whether iPAH patients exhibit underlying G6PD alterations in erythrocytes. A cohort of 22 PAH patients and 8 non-PAH patients were recruited for this study. DNA isolated from Peripheral Blood Mononuclear Cells (PBMC) was used for detection of mutations in the coding region of the G6PD gene. RNA isolated from PBMC was used for determination of G6PD mRNA expression level. G6PD activity was measured in Red Blood Cell (RBC) pellets. Three patients had missense mutations in G6PD (Val291Met, Asn126Asp, Asp194Glu), however, only one mutation (Val291Met) results in a severe G6PD deficiency. A single patient with mutation (Asn126Asp) showed a 21% decrease in G6PD activity, two subjects showed G6PD deficiency without mutations, and one patient had a decreased level of G6PD mRNA and reduced enzyme levels. This study demonstrates that a moderate decrease in G6PD activity is associated with PAH. Screening for G6PD activity and mutations in the G6PD gene may provide early detection of individuals predisposed to PAH.

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“…After the administration of extract these enzyme activities were significantly increased by increased secretion of insulin 29 . Pancreatic β-cell regenerative effect of this extract was noted in this study by the rise in serum insulin as well as recovery in volume and diameter of islets found in microphotography.…”

Section: Discussionmentioning

confidence: 95%

“…Glucose-6-phosphate dehydrogenase is an important enzyme in pentose phosphate pathway for maintenance of normal blood glucose level. Activity of this enzyme decreases significantly in diabetic rats as this enzyme is also under the control of insulin 29 and STZ destroy β-cell of pancreas 30 . This result also supported by our previous work 13 .…”

Section: Discussionmentioning

confidence: 99%

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2018

IJPSR

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The present study was conducted to investigate protective effect of hydro-methanolic (2:3) extract of leaf of Abroma augusta in streptozotocin induced diabetic male albino rat. For this purpose animals were divided into three treatment groups (n = 6): control group, STZ induced diabetic group (STZ 4 mg / 0.1 ml citrate buffer/ 100 g body weight), hydro-methanolic extract of leaf of Abroma augusta treated group (20 mg / 0.5 ml distilled water/100 g body weight). Treatment was conducted for 28 days. Blood glucose, serum insulin, glycated haemoglobin levels along with activities of hepatic carbohydrate metabolic enzymes, hexokinase and glucose-6-phosphate dehydrogenase were assed in all experimental groups. Oxidative stress markers like catalase, peroxidase enzyme activities, conjugated diene and thiobarbituric acid reactive substance levels in liver were measured. All these parameters were significantly recovered towards the control level after the treatment of hydro-methanolic extract. Pancreatic islet diameter, count of islet and number of cells in islets also decreased in diabetic animals. Treatment of these animals with extract resulted significant recovery towards the control level. Serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase were assed in all the groups and it has been revealed that the said extract has no metabolic toxicity in general. From this study it may be concluded that hydro-methanolic extract of leaf of A. augusta has protective effects on diabetes induced complications.

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Elevation of Glucose 6-Phosphate Dehydrogenase Activity Induced by Amplified Insulin Response in Low Glutathione Levels in Rat Liver

Cited by 8 publications

References 35 publications

Enzymatic Changes in Red Blood Cells of Diamond-Blackfan Anemia

Enzymatic Changes in Red Blood Cells of Diamond-Blackfan Anemia

New cases of Glucose-6-Phosphate Dehydrogenase deficiency in Pulmonary Arterial Hypertension

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