Elevation of Intracellular cAMP Levels by Dominant Active Heterotrimeric G Protein Alpha SubunitsScGP-AandScGP-Cin Homobasidiomycete,Schizophyllum commune

Yamagishi, Kazumasa; Kimura, Toshiyuki; Suzuki, Masahiro; Shinmoto, Hiroshi; Yamaki, Ko-ji

doi:10.1271/bbb.68.1017

Cited by 18 publications

(30 citation statements)

References 34 publications

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“…Yamagishi et al could also show that transformation with a dominantactive Ras allele rendered the resulting transformants slow growing in monokaryons. They did not observe growth reduction in dikaryons and, in contrast to our results, observed fewer instead of higher amounts of fruitbody initials (87). A possible explanation for these obvious and unexpected discrepancies in phenotypes could be that expression of Ras Q61L in the transformants was under control of a strong and developmentally regulated promoter of the aerial mycelium-specific hydrophobin Sc3 (3,64).…”

Section: Discussioncontrasting

confidence: 99%

“…However, differences in cAMP levels between the ⌬gap1 mutant and the wild type were not observed in either monokaryons or dikaryons (data not shown). In accordance with this, Yamagishi et al (87) did not detect changes in cAMP level in strains expressing a dominant-active Ras Q61L allele. Yamagishi et al could also show that transformation with a dominantactive Ras allele rendered the resulting transformants slow growing in monokaryons.…”

Section: Discussionmentioning

confidence: 56%

“…In addition, suppression of hyphal knot formation, the initial step of fruitbody development, and an altered hyphal growth pattern have been observed for C. cinereus strains expressing a dominant-active allele of ras (U. Kües, unpublished data). Recently, expression of the dominant active Ras Q61L allele has been reported for S. commune to result in the reduction of monokaryotic growth rate and fruitbody initiation (87).…”

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Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

et al. 2006

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The white rot fungus Schizophyllum commune is used for the analysis of mating and sexual development in homobasidiomycete fungi. In this study, we isolated the gene gap1 encoding a GTPase-activating protein for Ras. Disruption of gap1 should therefore lead to strains accumulating Ras in its activated, GTP-bound state and to constitutive Ras signaling. Haploid ⌬gap1 monokaryons of different mating types did not show alterations in mating behavior in the four different mating interactions possible in fungi expressing a tetrapolar mating type system. Instead, the growth rate in ⌬gap1 monokaryons was reduced by ca. 25% and ca. 50% in homozygous ⌬gap1/⌬gap1 dikaryons. Monokaryons, as well as homozygous dikaryons, carrying the disrupted gap1 alleles exhibited a disorientated growth pattern. Dikaryons showed a strong phenotype during clamp formation since hook cells failed to fuse with the peg beside them. Instead, the dikaryotic character of the hyphae was rescued by fusion of the hooks with nearby developing branches. ⌬gap1/⌬gap1 dikaryons formed increased numbers of fruitbody primordia, whereas the amount of fruitbodies was not raised. Mature fruitbodies formed no or abnormal gills. No production of spores could be observed. The results suggest Ras involvement in growth, clamp formation, and fruitbody development.The homobasidiomycetous white-rot fungus Schizophyllum commune has been used as a model system for the investigation of mating and sexual development for decades since it can be grown from spore to spore through its entire life cycle within 14 days on defined media, and it shows easily distinguished phenotypes for a tetrapolar mating behavior (32, 53). The tetrapolar mating system consists of two sets of mating type genes. The A mating type genes encode homeodomain transcription factors that are assumed to directly regulate gene expression (39,67). A multiallelic pheromone/receptor system is encoded by the B genes. Homology to the yeast pheromone system has led to the expectation that mating is in part controlled by a mitogen-activated protein kinase signal transduction cascade that is activated after stimulation of the G protein-coupled pheromone receptor (20,76,82).A fully compatible mating between two strains of S. commune occurs when both differ in their A and B gene specificities (A B ). The specificity of a locus is defined by a lack of activation of downstream developmental processes after crossing two strains with identical specificities in their mating type genes (20). Several steps of subsequent development can be distinguished. After cell fusion, septal breakdown and fast nuclear migration allow reciprocal nuclear exchange between the two mates. Migrant and resident nuclei pair, and dikaryotic hyphal tips are established. Subsequent conjugate nuclear division is accompanied by formation of clamp connections. Clamp connections are short, backwardly directed branches that fuse with the subapical cell and provide a bypass for one of the nuclei produced during synchronous division of the dikaryon, en...

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 56%

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Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

et al. 2006

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“…In Schizophyllum commune, the genes of G isoforms ScGP-A and ScGP-C were point-mutated to be the defect of the GTPase activity (dominant activation) by Yamagishi et al 26) Upon transformation using the mutated genes, the S. commune transformants increased the intracellular cAMP levels and altered morphological development. 26) We used this protocol to construct cAMP overproducing strains in P. ostreatus.…”

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“…26) We used this protocol to construct cAMP overproducing strains in P. ostreatus. All the primers used in cloning and vector construction are listed in Supplemental Table S1 (see Biosci.…”

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The White-Rot FungusPleurotus ostreatusTransformant Overproduced Intracellular cAMP and Laccase

Yao

Sakamoto

Honda

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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The Emergence of Fruiting Bodies in Basidiomycetes

Wösten

Wessels

The Mycota

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Elevation of Intracellular cAMP Levels by Dominant Active Heterotrimeric G Protein Alpha SubunitsScGP-AandScGP-Cin Homobasidiomycete,Schizophyllum commune

Cited by 18 publications

References 34 publications

Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

The White-Rot FungusPleurotus ostreatusTransformant Overproduced Intracellular cAMP and Laccase

The Emergence of Fruiting Bodies in Basidiomycetes

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