Elevation of serum soluble E‐selectin and VCAM‐1 in severe asthma

Hamzaoui, A.; Ammar, J.; F, el Mekki; Borgi, O; Ghrairi, H.; M, Ben Brahim; Hamzaoui, K

doi:10.1080/09629350120102361

Cited by 16 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have confirmed similarities in baseline serum levels of P-selectin in asymptomatic patients with mild asthma and in volunteers, findings which are in agreement with results from a recent study [16]. We have now also shown that repeated inhalations of salbutamol lowered P-selectin levels, and in agreement with well-known drug effects, also improved lung function in the asthmatics.…”

Section: Discussionsupporting

confidence: 81%

“…Short-acting β-2 agonists can, in vitro, inhibit expression of CAMs such as CD11b and L-selectin, and also inhibit the production of leukotrienes and eosinophil peroxides [16]. A similar decrease in serum markers in subjects with no asthmatic inflammation would theoretically speak against the decrease being solely a result of an anti-inflammatory effect of salbutamol.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Decreased Serum Levels of P-Selectin and Eosinophil Cationic Protein in Patients with Mild Asthma after Inhaled Salbutamol

Sjöswärd

Uppugunduri²,

Schmekel³

2004

Respiration

View full text Add to dashboard Cite

Background: Asthma is a chronic inflammatory disease of the airways associated with selective recruitment of activated eosinophils. P-selectin, a cell adhesion molecule, may be an important controller of the inflammation by mediating selective eosinophil cell influx to the lung. Serum levels of eosinophil cationic protein (ECP) have been used as a marker of eosinophil inflammation, and indirectly as a marker of disease activity of asthma. ECP levels may not be elevated in some patients with asthma, and this fact prompted us to search for additional surrogate markers for monitoring disease activity in asthma. Objectives: To evaluate whether repeated inhalations of salbutamol, a β-2-receptor agonist used for bronchodilation, would lead to reduced serum levels of P-selectin and/or ECP. Methods: Fourteen patients with asymptomatic mild stable asthma were enrolled into a randomised crossover study. Salbutamol was inhaled three times every 3 h. Blood was sampled 4 h after the last inhalation. Nine non-treated healthy volunteers served as control subjects. Serum ECP and P-selectin levels were measured using radioimmunoassay and ELISA, respectively. Results: P-selectin and ECP levels in serum obtained from asymptomatic asthmatics were close to those of the volunteers, and inter-day variability tended to be lower for levels of P-selectin than for ECP. Significant decreases of P-selectin (p = 0.01) and ECP (p = 0.03) were recorded after salbutamol inhalation. There was no association between the changes in ECP and P-selectin levels in serum. Conclusions: We conclude that decreases in P-selectin and ECP may have different kinetics, suggesting different pathways of action of salbutamol. We judge that P-selectin may be used as a sensitive marker in mild asthma.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Decreased Serum Levels of P-Selectin and Eosinophil Cationic Protein in Patients with Mild Asthma after Inhaled Salbutamol

Sjöswärd

Uppugunduri²,

Schmekel³

2004

Respiration

View full text Add to dashboard Cite

show abstract

“…sVCAM-1 binds with α4β1 integrins and alters lymphocyte functions and angiogenesis (48,49). sVCAM-1 levels are elevated in biological fluids in various diseases, including synovial fluids from patients with rheumatoid arthritis (50), cerebrospinal fluid from patients with active multiple sclerosis (51) and alveolar lining fluid from patients with eosinophilic pneumonia and asthma (52). However, the physiological functions of sVCAM-1 are unknown.…”

Section: Discussionmentioning

confidence: 99%

A Critical Role for P2X7 Receptor–Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury

Mishra

Guo

Zhang

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

Pulmonary neutrophils are the initial inflammatory cells that are recruited during lung injury and are crucial for innate immunity. However, pathological recruitment of neutrophils results in lung injury. The objective of this study is to determine whether the novel neutrophil chemoattractant, soluble VCAM-1 (sVCAM-1), recruits pathological levels of neutrophils to injury sites and amplifies lung inflammation during acute lung injury. The mice with P2X7 receptor deficiency, or treated with a P2X7 receptor inhibitor or anti-VCAM-1 antibodies were subjected to a clinically relevant two-hit LPS and mechanical ventilation-induced acute lung injury. Neutrophil infiltration and lung inflammation were measured. Neutrophil chemotactic activities were determined by a chemotaxis assay. VCAM-1 shedding and signaling pathways were assessed in isolated lung epithelial cells. Antibody neutralization of sVCAM-1 or deficiency or antagonism of P2X7R reduced neutrophil infiltration and proinflammatory cytokine levels. The ligands for sVCAM-1 were increased during acute lung injury. sVCAM-1 had neutrophil chemotactic activities and activated alveolar macrophages. VCAM-1 is released into the alveolar airspace from alveolar epithelial type I cells through P2X7 receptor-mediated activation of the metalloproteinase ADAM-17. In conclusion,sVCAM-1 is a novel chemoattractant for neutrophils and an activator for alveolar macrophages. Targeting sVCAM-1 provides a therapeutic intervention that could block pathological neutrophil recruitment, without interfering with the physiological recruitment of neutrophils, thus avoiding the impairment of host defenses.

show abstract

“…Multiple lines of evidence have shown the production of sVCAM‐1 in the circulation and inflammatory sites of asthmatics. The plasma level of sVCAM‐1 in asthmatic patients (753 ± 60.8 ng/ml) is higher than that of healthy controls (580 ± 70.7 ng/ml) (10). Koizumi et al.…”

Section: Discussionmentioning

confidence: 99%

“…Adhesion molecules, including VCAM‐1, undergo proteolytic cleavage, which releases the soluble ectodomain from the cell surface. Soluble forms of adhesion molecules are increased in the sputum (9), serum (10, 11), and bronchoalveolar lavage fluid (BALF) (12) of asthmatic patients. Elevated levels of soluble adhesion molecules reflect the activity of asthma; therefore, these molecules can be useful inflammatory markers (10, 11).…”

mentioning

confidence: 99%