ELFA IgG anti-Toxoplasma y PCR anidada para el diagnóstico de toxoplasmosis en mujeres gestantes de Sincelejo, Colombia

Blanco, Pedro; Assia, Yulenis Margarita; Montero, Yina; Orozco, Kelly Estella

doi:10.1016/s0123-9392(11)70739-1

Cited by 5 publications

(6 citation statements)

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“…DNA of T. gondii was detected in 10.1% of amniotic fluid by amplification of the B1 gene in positive samples of mothers with serological criteria for acute toxoplasmosis in Bogotá [ 55 ]. PCR on maternal blood samples is not a confirmatory test of fetal infection; nevertheless, a PCR-B1 assay described a positivity of 12% in blood samples of positive pregnant women from Sincelejo [ 56 ]. Moreover, a serologic test (ELISA ELFA) showed in the same location seroconversion of 2% during gestational control and neonatal mortality [ 33 ] and exposed the burden of congenital toxoplasmosis in the Caribbean region.…”

Section: Reviewmentioning

confidence: 99%

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

et al. 2014

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This paper summarizes prevalence of Toxoplasma gondii in humans and animals and associated correlates of infection, clinical spectrum of disease in humans, and genetic diversity of T. gondii isolates from Colombia. Recent studies, especially in the states of Antioquia, Quindío and Cundinamarca, indicate that toxoplasmosis is a major public health problem. Approximately half of the women of child bearing age have T. gondii antibodies, and the clinical disease in congenitally infected children is more severe than in Europe. Limited studies indicate that the strains of T. gondii from Colombia are genetically and phenotypically different than in Europe and North America. However, epidemiological factors, such as the involvement of domestic and/or wild animals in transmission, the distribution of strain diversity by natural geographic regions, and the variation in risk factors between regions that are associated with human infection in Colombia, remain unknown. Areas of research for the future are outlined. This review should be of interest to biologists, veterinarians, physicians, and parasitologists.

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Section: Reviewmentioning

confidence: 99%

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

et al. 2014

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“…In recent years, it has been applied to confirm congenital toxoplasmosis with a sensitivity of 72 to 85% in the third month of life and, when combined with serology, the sensitivity increases to 94% with a specificity of 100%. Therefore, IgG-WB can be used for early diagnosis in combination with other markers of the infection, such as anti-T. gondii IgM and rep 529 32 .…”

Section: Discussionmentioning

confidence: 99%

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

Souza

Siqueira

Ribeiro

et al. 2023

Rev. Inst. Med. trop. S. Paulo

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Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent is Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it is of utmost importance to perform a diagnosis with reproducible techniques in order to obtain a good prognosis. The aim of this review was to analyze the efficiency of toxoplasmosis diagnostic techniques based on sensitivity and specificity results. Five research platforms in English language were used (Eric, Elsevier, Google Scholar, PubMed and SciELO), which contained data on the diagnosis of toxoplasmosis. The search and selection were performed for studies published prior to June 2021. The search resulted in the inclusion of 13 articles published from 2005 to 2020. The data revealed the use of different samples in the standardization of techniques such as serum, total blood, colostrum and amniotic fluid. The flow cytometry, lateral flow immunoassay and qPCR techniques showed 100% sensitivity, whereas the ELISA, western blotting, qPCR and RE-LAMP techniques achieved 100% specificity. Significantly, the qPCR and LAMP techniques were more accurate when the likelihood ratio was assessed. The meta-analysis identified that ISAGA and western blotting have low sensitivity values and LIASON, ELFA and ELISA, using a silica bioconjugate, also have low specificity values. It was noted that a wide range of methods have high values of sensitivity and specificity. Therefore, the choice of the method will be based on the conditions and its financial viability.

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“…The aim of this study was to evaluate the use of serological, molecular and imaging methods to monitor the status and symptoms of patients clinically diagnosed with acute ocular toxoplasmosis. The B1 gene of T. gondii was used as target for molecular analysis since it has been described as more appropriated to characterize clinical samples in Brazil in contrast with observations from other countries [ 24 , 28 , 30 , 43 – 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…The growing interest in the investigation of toxoplasmosis has helped to develop strategies for early laboratory diagnosis and clinical intervention [ 24 – 30 ]. Moreover, it has been found that imaging tests such as fundus photography, fluorescein angiography and optical coherence tomography (OCT) help in the assessment, registration and documentation of eye disease, thereby showing whether it is in the acute phase or quiescent, and can also be used to monitor eye involvement in respect to T. gondii infection [ 3 , 31 – 39 ].…”

Section: Introductionmentioning

confidence: 99%

A Brazilian report using serological and molecular diagnosis to monitoring acute ocular toxoplasmosis

et al. 2015

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BackgroundToxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging. The aim of this study was to evaluate the use of serology and molecular methods to monitor acute OT in immunocompetent patients during treatment.MethodsFive immunocompetent patients were clinically diagnosed with acute OT. The clinical evaluation was performed by ophthalmologic examination using the Early Treatment Diabetic Retinopathy Study, best-corrected visual acuity, slit lamp biomicroscopy, fundoscopic examination with indirect binocular ophthalmoscopy color fundus photography, fluorescein angiography and spectral optical coherence tomography (OCT). Serology were performed by ELISA (IgA, IgM, IgG) and confirmed by ELFA (IgG, IgM). Molecular diagnoses were performed in peripheral blood by cPCR using the Toxoplasma gondiiB1 gene as the marker. Follow-up exams were performed on day +15 and day +45.ResultsOnly five non-immunocompromised male patients completed the follow up and their data were used for analysis. The mean age was 41.2 ± 11.3 years (median: 35; range 31–54 years). All of them were positive for IgG antibodies but with different profiles for IgM and IgA, as well as PCR. For all patients the OCT exam showed active lesions with the inner retinal layers being abnormally hyper-reflective with full-thickness disorganization of the retinal reflective layers, which assumed a blurred reflective appearance and the retina was thickened.ConclusionsThe presence of IgA and IgM confirmed the acute infection and thus was in agreement with the clinical evaluation. Our results show the adopted treatment modified the serological profile of IgM antibodies and the PCR results, but not the IgG and IgA antibodies and that imaging is a good tool to follow-up patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-015-1650-6) contains supplementary material, which is available to authorized users.

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ELFA IgG anti-Toxoplasma y PCR anidada para el diagnóstico de toxoplasmosis en mujeres gestantes de Sincelejo, Colombia

Cited by 5 publications

References 9 publications

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

A Brazilian report using serological and molecular diagnosis to monitoring acute ocular toxoplasmosis

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