Elicitation and Suppression of Isoflavones and Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Cultures

Barz, Wolfgang; Bless, W.; Daniel, Susanne; Gunia, W.; Hinderer, Walter; Jaques, U.; Keßmann, Helmut; Meier, D.; Tiemann, K.; Wittkampf, Uta

doi:10.1007/978-3-642-74551-5_23

Cited by 13 publications

(4 citation statements)

References 20 publications

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“…If medicarpin inhibits VA fungal growth or differentiation, this would explain the requirement for suppression of the constitutive levels of IFR in M. truncatula roots. Active suppression of defence mechanisms by fungal plant pathogens has been previously reported (Barz et al, 1989;Doke et al, 1979;Yamada et al, 1989) and suppression of gene expression has also been suggested to occur in bean mycorrhizae where, in contrast to the results observed here, CHI transcripts decreased during colonization (Lambais and Mehdy, 1993).…”

Section: Spatial Patterns Of Gene Expression In Mycorrhizae 17supporting

confidence: 58%

Spatial patterns of expression of flavonoid/isoflavonoid pathway genes during interactions between roots of Medicago truncatula and the mycorrhizal fungus Glomus versiforme

Harrison¹,

Dixon

1994

The Plant Journal

200

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SummaryA combination of Northern blot analysis and in situ hybridization was used to measure and localize changes in the levels of transcripts encoding five enzymes of phenylpropanoid/flavonoid/isoflavonoid metabolism in Medicago truncatula roots following colonization with the mycorrhizal fungus Glomus versiforme. In colonized roots, elevated levels of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) transcripts were detected specifically in the cortical cells containing arbuscules. This localization was discrete, and elevated levels of transcripts were not observed in adjacent, non-colonized cells. In contrast, isoflavone reductase (IFR) transcripts, which were detected at relatively high levels in the cortical cells of non-colonized roots, were almost undetsctable in the colonized cortical cells containing arbuscules and were also lowered in adjacent calls. The expression of chalcone isomerase (CHI) and isoliquiritigenin 2'-O-methyltransferase (ChalOMT) was unaffected by colonization, and the tissue-specific patterns of gene expression were the same as in noncolonized roots. It is concluded that the establishment of the mycorrhizal interaction results in cell type-specific differential expression of gemm of phenylpropanoid/flavonold/isoflavonoid biosynthesis, which may be causally related to arbuscule development.

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Section: Spatial Patterns Of Gene Expression In Mycorrhizae 17supporting

confidence: 58%

Spatial patterns of expression of flavonoid/isoflavonoid pathway genes during interactions between roots of Medicago truncatula and the mycorrhizal fungus Glomus versiforme

Harrison¹,

Dixon

1994

The Plant Journal

200

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“…Recently, Konoshima et al (1988) have also reported a potent antitumour promoter activity of formononetin and other isoflavonoids. On the other hand, formononetin plays a key role as an intermediary in the biosynthesis of other isoflavonoids, particularly the phytoalexin type (Barz et al 1989). …”

Section: Introductionmentioning

confidence: 99%

The effect of cultural conditions on the accumulation of formononetin by suspension cultures of Glycyrrhiza glabra

Arias-Castro

Seragg

Rodríguez-Mendiola

1993

Plant Cell Tiss Organ Cult

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Secondary product accumulation by suspension cultures of Glycyrrhiza glabra has been studied. The cultures did not accumulate either glycyrrhizin of glycyrrhetic acid but did produce the isoflavonoid formononetin. The effect of initial pH, sucrose concentrations, medium composition, auxin and cytokinins on formononetin accumulation was studied. Initially formononetin accumulation was nongrowth related but this changed to growth associated when the medium was modified.

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“…Vol. 94,1990 noids which accumulate are derivatives of precursors of medicarpin rather than the phytoalexin itself (3). In this system, formononetin-7-O-glucoside-6"-O-malonate can exhibit rapid turnover, whereas the corresponding conjugate of 5-hydroxy-formononetin appears to be metabolically inert (14).…”

Section: Discussionmentioning

confidence: 99%

Stress Responses in Alfalfa (Medicago sativa L.)

Keßmann

Edwards²,

Geno³

et al. 1990

Plant Physiol.

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The isoflavonoid conjugates medicarpin-3-0-glucoside-6"-Omalonate (MGM), afrormosin-7-O-glucoside (AG), and afrormosin-7-O-glucoside-6"-O-malonate (AGM) were isolated and characterized from cell suspension cultures of alfalfa (Medicago sativa L.), where they were the major constitutive secondary metabolites. They were also found in alfalfa roots but not in other parts of the plant. The phytoalexin medicarpin accumulated rapidly in suspension cultured cells treated with elicitor from Colletotrichum lindemuthianum, and this was subsequently accompanied by an increase in the levels of MGM. In contrast, net accumulation of afrormosin conjugates was not affected by elicitor treatment.Labeling studies with [14CJphenylalanine indicated that afrormosin conjugates were the major de novo synthesized isoflavonoid products in unelicited cells. During elicitation, [14C]phenylalanine was incorporated predominantly into medicarpin, although a significant proportion of the newly synthesized medicarpin was also conjugated. Treatment of 14C-labeled, elicited cells with L-a-aminooxy-,#-phenylpropionic acid, a potent inhibitor of PAL activity in vivo, resulted in the initial appearance of labeled medicarpin of very low specific activity, suggesting that the phytoalexin could be released from a preformed conjugate under these conditions. Our data draw attention to the involvement of isoflavone hydroxylases during the constitutive and elicitor-induced accumulation of isoflavonoids and their conjugates in alfalfa cell cultures.

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Elicitation and Suppression of Isoflavones and Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Cultures

Cited by 13 publications

References 20 publications

Spatial patterns of expression of flavonoid/isoflavonoid pathway genes during interactions between roots of Medicago truncatula and the mycorrhizal fungus Glomus versiforme

Spatial patterns of expression of flavonoid/isoflavonoid pathway genes during interactions between roots of Medicago truncatula and the mycorrhizal fungus Glomus versiforme

The effect of cultural conditions on the accumulation of formononetin by suspension cultures of Glycyrrhiza glabra

Stress Responses in Alfalfa (Medicago sativa L.)

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