Elicitation directed growth and production of steviol glycosides in the adventitious roots of Stevia rebaudiana Bertoni

Improved Production of Industrially Important Essential Oils Through Elicitation in the Adventitious Roots of Artemisia amygdalina

“…Similarly lower growth response was observed in response to Me-J during AR suspension culture in A. scoparia ; a medicinally important Artemisia plant species [32]. Contrarily, Me-J has positively influenced AR growth in context of AR induction and biomass formation in medicinally important plants, A. bracteosa and S. rebaudiana [31,44]. The growth-stimulating effects of Me-J and PAA as chemical elicitors in AR cultures have not been well explored.…”

“…It is a common pattern that cytokinins, preferably benzyl aminopurine (BA), promotes shoot organogenesis in explants. Likewise auxins, notably NAA usually results in root regeneration during in vitro cultures [30,31]. In several medicinal plants, NAA has been reported for its highest activity in AR formation [17].…”

“…The prospects of bioprocess technology include production of biomass and enhancement of medicinally active metabolites; those may not be found in the counterpart natural plants. Moreover this technology provides a suitable model system to study and understand the plant cell cycle, effects of plant growth regulators or elicitors on the growth, physiological responses and secondary metabolism of plant cell during in vitro growth [18,31,43]. In this study, AR of A. amygdalina grown over solid media containing 1.0 mg/L NAA and 3% sucrose were taken and were transiently elicited (pre-treatment) with different elicitors for different time periods; after that, approximately 2 g/L of AR pretreated with each elicitor were transferred into liquid MS media containing 1.0 mg/L NAA and 3% sucrose, following incubation on rotary shaker for a 30-day culture period.…”

“…Among all the elicitor- treatments, melatonin (Mel: 1.0 mg/L), phenyl acetic acid (PAA: 0.5 mg/L) and methyl jasmonate (Me-J: 0.5 mg/L) resulted in higher AR growth of 80%, 60% and 80%, respectively, in shake flasks (Figure 2a). No study has previously reported on the impacts of chemical elicitors on the in vitro cell growth and development in A. amygdalina; however, all the elicitors employed in the current study have been well explored for their effects on growth in different plant cell culture studies in a variety of medicinal plants [21,31,44]. Uptake of methyl jasmonate by plant cell results in the mutilation of the plant cell outer layer; acting as a signal compound, it triggers the plant cell biochemical pathways through reprogramming common attributes of the plant cell, such as plasticity and totipotency.…”

“…However, recently, both Me-J and PAA have been reported for their positive effects on induction and biomass accumulation of AR in a high valued medicinal plant Ajuga bracteosa [44]. Both Me-J and PAA can initiate a cascade of different metabolic events in plant cell as a result of the stress condition induced by these elicitors, consequently resulting in induction of AR and biomass formation in explants [21,31].…”