Elicitation of Hyoscyamine Production in Datura stramonium L. Plants Using Tobamoviruses

Abstract

Datura stramonium L. produces tropane alkaloids, and the hyoscyamine is dominant among them. Hyoscyamine is produced by hairy root cultures in vitro derived from native plants or plants with the genetically modified biosynthetic pathway for hyoscyamine. A common procedure is extraction from cultivated plants. Elicitors for increased production can be used in both cases. Live viruses are not well known for use as elicitors, therefore, D. stramonium plants grown in soil were artificially infected with the tobamoviruses Pepper mild mottle virus (PMMoV), Tomato mosaic virus (ToMV), and Tobacco mosaic virus (TMV). Differences in the content of hyoscyamine were between capsules and roots of infected and non-infected plants. Elicitation increased content of hyoscyamine in capsules 1.23–2.34 times, compared to the control. The most effective viruses were PMMoV and ToMV (isolate PV143), which increased content to above 19 mg/g of fresh weight of a capsule. The effect of each virus elicitor was expressed also in hyoscyamine content in roots. Elicited plants contained 5.41–16.54 times more hyoscyamine in roots compared to non-elicited plants. The most effective elicitor was ToMV SL-1, which raised production above 20 mg/g fresh weight of roots. It has been shown that tobamoviruses can be used as biotic elicitors.

Metabolic Effects of Elicitors on the Biosynthesis of Tropane Alkaloids in Medicinal Plants

“…Mihálik et al used three tobacco mosaic viruses (PMMoV, TMV, and ToMV) to artificially infect Datura stramonium through in vivo elicitation. They discovered that the hyoscyamine content was significantly higher compared to in vitro induction or non-elicited capillary root cultures, which illustrates the potential value of viral elicitation [65]. In order to clearly demonstrate the role of each biotic elicitor in TAs biosynthesis, a table (Table 1) is provided, which includes all the relevant elements.…”

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in-vitro cultures

“…It has been well-documented that plant species belonging to the Datura genus possess a variety of secondary metabolites, such as alkaloids, terpenoids, and avonoids, which have valuable medicinal and therapeutic applications [9,10]. For this reason, various strategies, such as applications of chemical [11], physical [10], and biotic elicitors [12] have been employed to stimulate the production of secondary metabolites of this plant under laboratory cultivation conditions. Despite the development of seed disinfection procedures, endogenic seed-borne contaminations restrict the e ciency of tissue or organ cultures of medicinal plants.…”

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in‐vitro cultures

“…[9] It has been well-documented that plant species belonging to the Datura genus possess a variety of secondary metabolites, such as alkaloids, terpenoids, and flavonoids, which have valuable medicinal and therapeutic applications. [10,11] For this reason, various strategies, such as applications of chemical, [12] physical, [11] and biotic elicitors [13] have been employed to stimulate the production of secondary metabolites of this plant under laboratory cultivation conditions. Despite developing seed disinfection procedures, endogenic seed-borne contaminations restrict the efficiency of tissue or organ cultures of medicinal plants.…”