1991
DOI: 10.1515/znc-1991-1-208
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Elicitor-Induced Formation of Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Suspension Cultures from Constitutive Isoflavone Conjugates upon Inhibition of Phenylalanine Ammonia Lyase

Abstract: U lrike M ack en b ro ck an d W olfgang Barz L ehrstuhl für Biochem ie d er Pflanzen, W estfälische W ilhelm s-U niversität, H in d en b u rg p latz 55, D -4400 M ü n ster, B undesrepublik D eutschland Z. N atu rfo rsch . 46c, 4 3 -5 0 (1991); received O cto b er 9, 1990 Cicer arietinum , Cell C u ltu re, E licitor, P tero carp an Phytoalexins, F o rm o n o n etin 7 -0 -g lu co sid e -6 "-0 -m a lo n a te A fter inhibition o f p h en y lalan in e am m o n ia lyase by L -a-am inooxy-ß-phenylpropionic acid, the … Show more

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Cited by 39 publications
(22 citation statements)
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“…The similarity of the responses observed in the alfalfa-G. intraradices interaction to those reported for the simultaneous addition of a PAL inhibitor and an elicitor to chickpea cell suspensions (Mackenbrock & Barz, 1991) supports the conclusion that Glomus intraradices elicits a plant defence response in alfalfa roots which is suppressed by the fungus while colonizing the root tissue. Harrison & Dixon (1993) supphed evidence that an AM fungus, G. versiforme, suppressed the host defence response in two Medicago species.…”
Section: Reduction Of Defence Responsessupporting
confidence: 78%
“…The similarity of the responses observed in the alfalfa-G. intraradices interaction to those reported for the simultaneous addition of a PAL inhibitor and an elicitor to chickpea cell suspensions (Mackenbrock & Barz, 1991) supports the conclusion that Glomus intraradices elicits a plant defence response in alfalfa roots which is suppressed by the fungus while colonizing the root tissue. Harrison & Dixon (1993) supphed evidence that an AM fungus, G. versiforme, suppressed the host defence response in two Medicago species.…”
Section: Reduction Of Defence Responsessupporting
confidence: 78%
“…However, when PAL was inhibited, the formononetin moiety of the conjugate was used for pterocarpan biosynthesis. Consumption of the isoflavone moiety for pterocarpan biosynthesis depended on the simultaneous effects of elicitation and the inhibition of PAL (Mackenbrock and Barz, 1991). In our system no elevation in medicarpin production was noted despite the increase in formononetin levels.…”
Section: Discussionmentioning
confidence: 51%
“…In chickpea cell-suspension cultures treated concomitantly with an elicitor and a PAL inhibitor, a metabolic linkage between isoflavone conjugate turnover and pterocarpan biosynthesis was evident (Mackenbrock and Barz, 1991). At the time of rapid pterocarpan formation, no quantitative change in the formononetin conjugate formononetin-7-0-(6"-O-malonylglycoside) was observed.…”
Section: Discussionmentioning
confidence: 99%
“…Constitutively accumulated glucosides are generally regarded as the stable, soluble storage forms of isoflavonoids and typically localized within the cell's central vacuole (Mackenbrock and Barz, 1991). The response to MeJA was in sharp contrast to that of YE, where many pathway precursors were unchanged and increases in afrormosin and medicarpin were correlated with the consumption or significant decreases in isoflavonoid conjugate levels, particularly the glucosides of formononetin and 2#-hydroxyformononetin.…”
Section: Differential and Elicitior-specific Induction Of Isoflavonoimentioning
confidence: 99%
“…The differential responses observed here are also consistent with results obtained in elicited chickpea cells treated with a PAL inhibitor. Chickpea cells treated with a fungal elicitor reportedly accumulate medicarpin via de novo biosynthesis, whereas cells elicited in the presence of the PAL inhibitor L-a-aminooxy-b-phenylpropionic acid utilized vacuolar pools of formononetin glucoside conjugates as precursors for medicarpin biosynthesis (Mackenbrock and Barz, 1991). In M. truncatula cell culture, MeJA appears to be acting in a similar manner and remobilizing vacuolar stores of phenylpropanoid glycosides to serve as precursors for medicarpin and afrormosin biosynthesis.…”
Section: Differential and Elicitior-specific Induction Of Isoflavonoimentioning
confidence: 99%