Elicitors and Their Roles in Plant Defence Against Pathogens Particularly Basidiomycetes

Hamid, Sathyapriya; Wong, Mui Yun

doi:10.1007/978-3-319-65079-1_14

Cited by 7 publications

(1 citation statement)

References 174 publications

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“…Various fungal-derived elicitor proteins, such as cell wall-degrading enzymes (CWDEs), toxins, and smallmolecule proteins, can activate the PTI of host plants upon recognition of speci c plant cell surface receptors, as reviewed by Hamid & Wong (2017). The increased production of reactive oxygen species (ROS) and reactive nitrogen intermediates in response to pathogen or elicitor treatment plays a vital role in plant defence responses, as they initiate intracellular signalling pathways that increase salicylic acid (SA) levels and activate systemic acquired resistance (SAR).…”

Section: Introductionmentioning

confidence: 99%

The elicitation of early defence responses and improvement of disease resistance in oil palm by extracellular proteins from Ganoderma boninense

Hamid,

Abdullah,

et al. 2024

Preprint

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Ganoderma boninense, a basidiomycete that causes basal stem rot (BSR) disease, threatens oil palm production and causes significant economic losses. An eco-friendly alternative to chemical BSR management is needed. This study investigated the impact of the extracellular protein sample from G. boninense on early defence responses and disease resistance in oil palm using histochemical, biochemical, and molecular approaches. The extracellular protein sample caused hypersensitive response (HR)-associated cell death, leading to rapid necrosis, and induced reactive oxygen species (ROS) production in oil palm leaf sections at 24 h post infiltration. Cell death was confirmed by trypan blue staining, and increased superoxide anion radicals and hydrogen peroxide were detected using nitroblue tetrazolium and 3′–3′ diaminobenzidine staining, respectively. The extracellular protein sample significantly increased the activities of superoxide dismutase (SOD) and guaiacol peroxidase (GPx) in the roots. The SOD and GPx activities were greater than 100 U mg− 1 FW and 16 U mg− 1 protein, respectively, at 12, 24, 48, and 72 hours post treatment (hpt). Additionally, respiratory burst oxidase homolog B (RbohB) expression increased 2.5-fold in treated roots at 12 hpt, indicating ROS accumulation and supporting HR induction by the extracellular protein sample. In a greenhouse trial, the application of the extracellular protein sample led to a 71.43% reduction in BSR incidence in oil palm nine months after inoculation. These findings demonstrate the potential of using extracellular protein sample from G. boninense as an effective and eco-friendly BSR management tool.

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Section: Introductionmentioning

confidence: 99%

The elicitation of early defence responses and improvement of disease resistance in oil palm by extracellular proteins from Ganoderma boninense

Hamid,

Abdullah,

et al. 2024

Preprint

View full text Add to dashboard Cite

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Bio-active Peptides: Role in Plant Growth and Defense

Pan

Agyei

Jeevanandam

et al. 2019

Natural Bio-Active Compounds

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CfPDIP1, a novel secreted protein of Colletotrichum falcatum, elicits defense responses in sugarcane and triggers hypersensitive response in tobacco

Ashwin

Barnabas

Sundar

et al. 2018

Appl Microbiol Biotechnol

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Colletotrichum falcatum, a hemibiotrophic fungal pathogen, causes one of the major devastating diseases of sugarcane-red rot. C. falcatum secretes a plethora of molecular signatures that might play a crucial role during its interaction with sugarcane. Here, we report the purification and characterization of a novel secreted protein of C. falcatum that elicits defense responses in sugarcane and triggers hypersensitive response (HR) in tobacco. The novel protein purified from the culture filtrate of C. falcatum was identified by MALDI TOF/TOF MS and designated as C. falcatum plant defense-inducing protein 1 (CfPDIP1). Temporal transcriptional profiling showed that the level of CfPDIP1 expression was greater in incompatible interaction than the compatible interaction until 120 h post-inoculation (hpi). EffectorP, an in silico tool, has predicted CfPDIP1 as a potential effector. Functional characterization of full length and two other domain deletional variants (CfPDIP1ΔN1-21 and CfPDIP1ΔN1-45) of recombinant CfPDIP1 proteins has indicated that CfPDIP1ΔN1-21 variant elicited rapid alkalinization and induced a relatively higher production of hydrogen peroxide (HO) in sugarcane suspension culture. However, in Nicotiana tabacum, all the three forms of recombinant CfPDIP1 proteins triggered HR along with the induction of HO production and callose deposition. Further characterization using detached leaf bioassay in sugarcane revealed that foliar priming with CfPDIP1∆1-21 has suppressed the extent of lesion development, even though the co-infiltration of CfPDIP1∆1-21 with C. falcatum on unprimed leaves increased the extent of lesion development than control. Besides, the foliar priming has induced systemic expression of major defense-related genes with the concomitant reduction of pathogen biomass and thereby suppression of red rot severity in sugarcane. Comprehensively, the results have suggested that the novel protein, CfPDIP1, has the potential to trigger a multitude of defense responses in sugarcane and tobacco upon priming and might play a potential role during plant-pathogen interactions.

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Elicitors and Their Roles in Plant Defence Against Pathogens Particularly Basidiomycetes

Cited by 7 publications

References 174 publications

The elicitation of early defence responses and improvement of disease resistance in oil palm by extracellular proteins from Ganoderma boninense

The elicitation of early defence responses and improvement of disease resistance in oil palm by extracellular proteins from Ganoderma boninense

Bio-active Peptides: Role in Plant Growth and Defense

CfPDIP1, a novel secreted protein of Colletotrichum falcatum, elicits defense responses in sugarcane and triggers hypersensitive response in tobacco

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