Eliminating female Anopheles arabiensis by spiking blood meals with toxicants as a sex separation method in the context of the sterile insect technique

Yamada, Hanano; Soliban, S. M.; Vreysen, Marc J. B.; Chadee, Dave D.; Gilles, J.

doi:10.1186/1756-3305-6-197

Cited by 27 publications

(29 citation statements)

References 24 publications

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“…For all other experiments, when more males were required, females were killed by offering fresh bovine blood meals spiked with Ivermectin (Ivomec) as was previously described by Yamada et al. ().…”

Section: Methodsmentioning

confidence: 99%

“…To determine the temperature and duration threshold for transport, males were separated from females using a mouth aspirator (John W Hock, Gainesville, FL, USA) within 24 h of emergence, after access to a sugar meal. For all other experiments, when more males were required, females were killed by offering fresh bovine blood meals spiked with Ivermectin (Ivomec) as was previously described by Yamada et al (2013).…”

Section: Mosquito Colony Rearingmentioning

confidence: 99%

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Optimised conditions for handling and transport of male Anopheles arabiensis: effects of low temperature, compaction, and ventilation on male quality

Culbert

Lees

Vreysen

et al. 2017

Entomologia Exp Applicata

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Developing optimal conditions for handling and transport of sterile male mosquitoes, prior to their release, is critical for the sterile insect technique (SIT) to be successful. No data currently exist for Anopheles arabiensis Patton (Diptera: Culicidae) concerning the effects of chilling at different temperatures and for different time lengths on subsequent survival. Additionally, it must be determined whether immobile mosquitoes are capable of producing metabolic heat, the maximum packing density in one release cassette during transport, and their toleration level to compaction. Male An. arabiensis were exposed to 2, 4, 6, and 10°C for 1, 4, 8, or 24 h. Survival was then monitored for 14 days and compared with untreated controls maintained at 25°C. Short-term (24 h) survival was assessed following immobilisation at 6°C for 6 h under compacted and non-compacted conditions and compared with non-immobilised controls. The experiment was repeated to assess long-term (1-14 days) survival with varying levels of ventilation. Metabolic heat was assessed in immobilised males (compacted and non-compacted) and compared with males maintained at 28°C for 2 h. The weight and volume of males were determined to guide the design of the release cassette. Male An. arabiensis were maintained at a temperature range of 4-10°C for 24 h without any significant negative effect on their survival. Compaction did not significantly affect survival; however, it fared better with increased ventilation. Immobile males did not produce any metabolic heat, even when compacted. This study identified initial parameters considered critical for the transport of sterile male An. arabiensis prior to release without any detrimental effect on their survival. Further investigation is required to assess the effects of combining these chosen treatments with irradiation. Additionally, the effect of immobilisation, compaction, and irradiation on the most critical parameter for SIT (male competitiveness) is the subject of future studies.

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Section: Methodsmentioning

confidence: 99%

Section: Mosquito Colony Rearingmentioning

confidence: 99%

Optimised conditions for handling and transport of male Anopheles arabiensis: effects of low temperature, compaction, and ventilation on male quality

Culbert

Lees

Vreysen

et al. 2017

Entomologia Exp Applicata

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“…Females can be provided with bloodmeals spiked with ivermectin at a concentration of 7.5 p.p.m. to ensure total elimination within 4 days (Yamada et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Development of a genetic sexing strain of Anopheles arabiensis for KwaZulu‐Natal, South Africa

Dandalo

Munhenga

Kaiser

et al. 2017

Medical Vet Entomology

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Abstract. An efficient sexing system is important for the release of sterile males for any control programme using the sterile insect technique. This study describes the development and characterization of a new genetic sexing strain from South Africa (GMK), needed for the planned implementation of such a programme in northern KwaZulu-Natal Province. The base colony used was a locally modified laboratory strain of Anopheles arabiensis containing a sex-linked gene conferring dieldrin resistance to male mosquitoes. Female A. arabiensis mosquitoes from northern KwaZulu-Natal were mated with these males and backcrossed to introduce the dieldrin resistance gene to the Y chromosome. The resulting strain therefore had an overall genotype representing the local population but with the Y chromosome containing the dieldrin resistance gene. Life-history characteristics, stability of the sex-linked resistance marker, and reduction in dieldrin waste were investigated. The strain showed semi-sterility exhibited by low egg hatch rates, faster development in the immature stages and longer adult survivorship compared with the parental strains. While the GMK strain carrying the dieldrin-resistant gene was successfully established, the stability of the gene is limited, requiring periodic purification. Dieldrin waste can be limited by treating many more eggs than currently recommended.

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“…Because the mutations Infected blood meals Ivermectin insecticide in blood meals An. arabiensis [72] Cas-9 and CRISPR guides in different lines…”

Section: Automated Sex-separation In Late Developmental Stagesmentioning

confidence: 99%

Sex Sorting for Pest Control: It’s Raining Men!

Lutrat

Giesbrecht

Marois

et al. 2019

Trends in Parasitology

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In the pursuit of better pest-and vector-control strategies, attention returns to an old proven technology, the sterile insect technique (SIT) and related insect population-suppression methods. A major obstacle for any of these approaches that involves the release of sterile males is the separation of males from females during the mass rearing stage, in order to improve the cost-efficiency of these methods and to prevent the release of biting and disease-vectoring females. This review describes recent sex-sorting developments in dipteran flies with an emphasis on assessing the suitability of these methods for large-scale rearing of male vectors for mass release. Sexing Is an Obstacle in Genetic Pest-control ProgramsHighlights Sexing Diptera represents a major obstacle to operationalizing vector-control methods based on the mass release of males, such as the sterile insect technique or the incompatible insect technique.

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Eliminating female Anopheles arabiensis by spiking blood meals with toxicants as a sex separation method in the context of the sterile insect technique

Cited by 27 publications

References 24 publications

Optimised conditions for handling and transport of male Anopheles arabiensis: effects of low temperature, compaction, and ventilation on male quality

Optimised conditions for handling and transport of male Anopheles arabiensis: effects of low temperature, compaction, and ventilation on male quality

Development of a genetic sexing strain of Anopheles arabiensis for KwaZulu‐Natal, South Africa

Sex Sorting for Pest Control: It’s Raining Men!

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