Elimination kinetic of recombinant somatotropin in bovine

Breton, Marie-Hélène Le; Rochereau-Roulet, Sandrine; Pinel, Gaud; Cesbron, Nora; Bizec, Bruno Le

doi:10.1016/j.aca.2008.09.003

Cited by 30 publications

(21 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The developed FCIA screening method features a long detection window of at least two weeks after the last rbST treatment. Unfortunately, LC/MS confirmation methods for rbST in blood can only detect rbST for a short period following administration (Le Breton, Rochereau-Roulet, Pinel, Cesbron, & Le Bizec, 2009) and in milk, no time course studies were done in rbSTtreated animals by these authors. Because of this, high frequency blood sampling would be necessary to ensure confirmation of rbST abuse by this LC/MS method.…”

Section: Storage Tank Milk Analysismentioning

confidence: 93%

Monitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach

Ludwig¹,

Smits²,

Bremer³

et al. 2012

Food Control

View full text Add to dashboard Cite

Section: Storage Tank Milk Analysismentioning

confidence: 93%

Monitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach

Ludwig¹,

Smits²,

Bremer³

et al. 2012

Food Control

View full text Add to dashboard Cite

“…Although biweekly injections containing slow-release formulations are used to prolong the presence of rbST in the cows' body, the protein levels in treated animals cannot be distinguished from the background level throughout the whole two-week inter-injection period and large inter-individual differences in blood rbST levels were reported [19], [20], [24]–[27]. Furthermore, rbST immunoassays were not capable to distinguish the almost identical recombinant and endogenous forms of bST [19], [20], [24], [25] and mass spectrometry-based methods on the other hand required very tedious sample preparation procedures [26], [27]. For screening of rbST in cattle, a few biomarker-based methods were developed, but focused on a single candidate biomarker only [4], [9], [28]–[30].…”

Section: Introductionmentioning

confidence: 99%

Multiple Protein Biomarker Assessment for Recombinant Bovine Somatotropin (rbST) Abuse in Cattle

Ludwig¹,

Smits²,

Veer³

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

Biomarker profiling, as a rapid screening approach for detection of hormone abuse, requires well selected candidate biomarkers and a thorough in vivo biomarker evaluation as previously done for detection of growth hormone doping in athletes. The bovine equivalent of growth hormone, called recombinant bovine somatotropin (rbST) is (il)legally administered to enhance milk production in dairy cows. In this study, first a generic sample pre-treatment and 4-plex flow cytometric immunoassay (FCIA) were developed for simultaneous measurement of four candidate biomarkers selected from literature: insulin-like growth factor 1 (IGF-1), its binding protein 2 (IGFBP2), osteocalcin and endogenously produced antibodies against rbST. Next, bovine serum samples from two extensive controlled rbST animal treatment studies were used for in vivo validation and biomarker evaluation. Finally, advanced statistic tools were tested for the assessment of biomarker combination quality aiming to correctly identify rbST-treated animals. The statistical prediction tool k-nearest neighbours using a combination of the biomarkers osteocalcin and endogenously produced antibodies against rbST proved to be very reliable and correctly predicted 95% of the treated samples starting from the second rbST injection until the end of the treatment period and even thereafter. With the same biomarker combination, only 12% of untreated animals appeared false-positive. This reliability meets the requirements of Commission Decision 2002/657/EC for screening methods in veterinary control. From the results of this multidisciplinary study, it is concluded that the osteocalcin – anti-rbST-antibodies combination represent fit-for-purpose biomarkers for screening of rbST abuse in dairy cattle and can be reliably measured in both the developed 4-plex FCIA as well as in a cost-effective 2-plex microsphere-based binding assay. This screening method can be incorporated in routine veterinary monitoring programmes: in the European Union for detection of rbST abuse and in the control of rbST-free dairy farms in the United States of America and other countries.

show abstract

“…Antibodies are important biomarkers due to their long half‐life and specificity . Although the formation of antibodies to a virtually endogenous protein – only one out of 191 amino acids on the N‐terminal end is replaced – seems surprising at first, it was observed in approximately 70 out of 90 rbST‐treated cows using an enzyme‐linked immunosorbent assay (ELISA) . During human growth hormone treatment, no antibody formation was observed in adults; however in children, antibody formation was observed in up to 68% of the target group .…”

Section: Introductionmentioning

confidence: 99%

Development of a flow cytometric immunoassay for recombinant bovine somatotropin‐induced antibodies in serum of dairy cows

Smits¹,

Bremer²,

Ludwig³

et al. 2011

Drug Testing and Analysis

View full text Add to dashboard Cite

Administration of recombinant bovine somatotropin (rbST) to enhance milk production in dairy cows is banned within the European Union. Therefore, methods for pinpointing rbST abuse are required. Due to the problematic detection of rbST itself in serum, methods are also focused on detecting changes in rbST-related biomarkers. In this study, a fast and easy-to-perform microsphere-based flow cytometric immunoassay (FCIA) for detection of rbST-induced antibodies in serum was developed. Until now, detection of rbST-induced antibodies was also problematic due to non-specific binding of serum proteins resulting in a high rate of false positive results. Therefore, five different sample preparation methods, i.e. dilution, octanoic acid precipitation, filtration, protein G purification, and a previously described generic FCIA sample preparation were critically compared to overcome non-specific binding to the microspheres. Only the generic FCIA sample pretreatment was effective in reducing non-specific binding. As a result, an absolute decision level for detecting rbST antibodies in serum of dairy cows was determined and its applicability was demonstrated. In accordance with biological expectations from literature, rbST antibodies were induced in three out of four rbST-treated dairy cows. These rbST-induced antibodies were successfully detected for up to 4 weeks after the last rbST treatment, whereas no false positive results were obtained for 27 untreated dairy cows. This is the first method, able to overcome the interference of serum proteins and therefore, can be applied with high confidence for screening unknown herds of cattle for rbST antibodies, an important biomarker for pinpointing at rbST abuse in cattle.

show abstract

Elimination kinetic of recombinant somatotropin in bovine

Cited by 30 publications

References 22 publications

Monitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach

Monitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach

Multiple Protein Biomarker Assessment for Recombinant Bovine Somatotropin (rbST) Abuse in Cattle

Development of a flow cytometric immunoassay for recombinant bovine somatotropin‐induced antibodies in serum of dairy cows

Contact Info

Product

Resources

About