Elimination of Apoptotic Granulosa Cells by Intact Granulosa Cells and Macrophages in Atretic Mature Follicles of the Guinea Pig Ovary.

Kasuya, Kiyoshi

doi:10.1679/aohc.60.175

Cited by 37 publications

(27 citation statements)

References 29 publications

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“…The outline of the antrum was ragged. Only a small number of dead cells or apoptotic bodies, however, were observed, and they may have been phagocytosed by neighboring intact granulosa cells, which was confirmed by electron microscopic observation (Logothetopoulos et al, 1995;Kasuya, 1997). Moreover, the follicular size and theca cells did not change in an obvious way, and the oocytes still seemed to be healthy.…”

Section: Stage I: Loose Connections In Granulosa Layersmentioning

confidence: 58%

“…In Stage I, some pyknotic cells and apoptotic bodies were interspersed among the healthy cells. It was accepted that these dead cells or apoptotic bodies were eliminated by macrophages or neighboring intact cells (Kasuya, 1997;Wu et al, 2004). Stages III and IV showed the course of apoptosis in its mildest form.…”

Section: Discussionmentioning

confidence: 99%

“…For guinea pigs, however, it has been difficult to provide uniform classification criteria because there are unique morphologic changes in the atretic process. The differentiation of theca cells (Logothetopoulos et al, 1995;Kasuya, 1997) is an example of one unique change. Fortunately, the process of follicular atresia was described in detail and divided into seven stages for the first time in 1995 (Logothetopoulos et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:There is a lack of appropriate classification criteria for the determination of atretic follicles in guinea pigs. In the present study, new criteria were established based on the latest morphologic criteria for cell death proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009. Ovaries of guinea pigs were sampled on different stages of estrous cycle, and the morphologic observations of atretic follicles were investigated in serial sections. The results showed that the process of follicular atresia could be classified into four continuous stages: (1) the granulosa layer became loose, and some apoptotic bodies began to appear; (2) the granulosa cells were massively eliminated; (3) the theca interna cells differentiated; and (4) the residual follicular cells degenerated. In addition, the examination revealed that these morphologic criteria were accurate and feasible. In conclusion, this study provides new criteria for the classification of atretic follicles in guinea pigs, and this knowledge can inform future research in the area.

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Section: Stage I: Loose Connections In Granulosa Layersmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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“…This process of atresia is currently regarded as being due to apoptosis, a genetically programmed cell death, of the granulosa cell mass and ultimately of the oocyte (Hughes & Gorospe, 1991 ;Tilly et al 1991). Several authors have claimed that macrophages are active in phagocytosing apoptotic (pyknotic) granulosa cells in atretic follicles in mice (Byskov, 1974) and other animals (Peluso et al 1980 ;Bukovsky et al 1993Bukovsky et al , 1995Kasuya, 1995Kasuya, , 1997.…”

Section: mentioning

confidence: 99%

“…Bukovsky et al identified macrophages immunohistochemically in rat (1993) andhuman (1995) ovaries. Kasuya (1995) reported that apoptotic granulosa cells were phagocytosed by ' large round cells ', which were identified as macrophages on the basis of acid phosphatase positivity in rabbits and positive immunoreactivity with an antimacrophage monoclonal antibody (MR-1) in guinea pigs (Kasuya, 1997).…”

Section: mentioning

confidence: 99%

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

et al. 2000

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We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other granulosa cells of normal appearance were seen in the region of the zona pellucida in contact with and apparently phagocytosing atrophic oocytes. Such granulosa cells were characterised by the presence of gap junctions with other cells and frequently contained annular gap junctions in the cytoplasm. To confirm the lack of involvement of macrophages in the process of follicular atresia and elimination, specially prepared ovarian sections were incubated with antimouse macrophage monoclonal antibodies (F4\80, Mac-1, Mac-2). None of the follicles examined showed positive immunoreactivity with these antibodies. Atretic follicles may shrink and eventually disappear from the ovary as a result of repeated apoptosis and phagocytosis by granulosa cells. There is no evidence for the presence or involvement of macrophages in the atretic follicles, at least in prereproductive mice as examined.

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Histomorphology and innate immunity during the progression of osteoarthritis: Does synovitis affect cartilage degradation?

Wang

Yang

et al. 2017

Journal Cellular Physiology

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Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological stages of the disease. In the present study, our aim was to assess changes in innate immunity during the pathological processes of OA in articular cartilage (AC) and the synovial membrane (SM), which are the major structures in joints, and to systematically examine the histological changes in AC and SM in mild, moderate and severe cases of OA, in order to further speculate about the manner in which the interactions of AC and SM are facilitated by innate immunity. Histological methods (including HE and Safranin O-fast green staining), immunofluorescent double staining, TUNEL stain, and Western blots were used to assess the morphological changes within AC and SM tissues in healthy and mild, moderate, or severe OA rats. Our results showed that the damage to AC and SM within the joints progressively worsened in different degrees during the course of the disease, and that the innate immune system was closely involved in the AC and SM during each stage of OA. These findings also confirmed that SM may affect the pathological changes in AC through the innate immune system, and therefore affect the progress of OA.

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Elimination of Apoptotic Granulosa Cells by Intact Granulosa Cells and Macrophages in Atretic Mature Follicles of the Guinea Pig Ovary.

Cited by 37 publications

References 29 publications

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

Histomorphology and innate immunity during the progression of osteoarthritis: Does synovitis affect cartilage degradation?

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