Elimination of Grapevine leafroll associated virus-3, Grapevine rupestris stem pitting associated virus and Grapevine virus A from a Tunisian Cultivar by Somatic Embryogenesis and Characterization of the

Bouamama-Gzara, Badra; Selmi, Ilhem; Chebil, Samir; Melki, Imène; Mliki, Ahmed; Ghorbel, Abdelwahed; Carra, A.; Carimi, Francesco; Mahfoudhi, N.

doi:10.5423/ppj.oa.06.2017.0122

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…This finding confirmed previous works conducted using other genotypes, in which diagnostic systems less effective than HTS and RT‐qPCR were adopted. In particular, GLRaV‐2 (Goussard et al ., 1991 ), GLRaV‐3 (Goussard et al ., 1991 ; Gambino et al ., 2006 ; Bouamama‐Gzara et al ., 2017 ; San Pedro et al ., 2017 ; Malenica et al ., 2020 ), GRSPaV (Gambino et al ., 2006 ; Bouamama‐Gzara et al ., 2017 ; Turcsan et al ., 2020 ), GFLV (Gambino et al ., 2009 ; San Pedro et al ., 2017 ; Malenica et al ., 2020 ), GFkV (San Pedro et al ., 2017 ; Malenica et al ., 2020 ; Turcsan et al ., 2020 ), GVA (Gambino et al ., 2006 ; Bouamama‐Gzara et al ., 2017 ) and GVT (Turcsan et al ., 2020 ) were efficiently removed from SC. In addition, in the case of GLRaV‐4 and GVD, our results represent the first report of sanitation obtained by SC in grapevine (Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

Grapevine virome and production of healthy plants by somatic embryogenesis

Nuzzo

Moine

Nerva

et al. 2022

Microbial Biotechnology

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Summary Grapevine ( Vitis spp.) is a widespread fruit tree hosting many viral entities that interact with the plant modifying its responses to the environment. The production of virus‐free plants is becoming increasingly crucial for the use of grapevine as a model species in different studies. Using high‐throughput RNA sequencing, the viromes of seven mother plants grown in a germplasm collection vineyard were sequenced. In addition to the viruses and viroids already detected in grapevine, we identified 13 putative new mycoviruses. The different spread among grapevine tissues collected in vineyard, greenhouse and in vitro conditions suggested a clear distinction between viruses/viroids and mycoviruses that can successfully be exploited for their identification. Mycoviruses were absent in in vitro cultures, while plant viruses and viroids were particularly accumulated in these plantlets. Somatic embryogenesis applied to the seven mother plants was effective in the elimination of the complete virome, including mycoviruses. However, different sanitization efficiencies for viroids and grapevine pinot gris virus were observed among genotypes. The absence of mycoviruses in in vitro plantlets, associated with the absence of all viral entities in somaclones, suggested that this regeneration technique is also effective to eradicate endophytic/epiphytic fungi, resulting in gnotobiotic or pseudo‐gnotobiotic plants.

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Section: Resultsmentioning

confidence: 99%

Grapevine virome and production of healthy plants by somatic embryogenesis

Nuzzo

Moine

Nerva

et al. 2022

Microbial Biotechnology

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“…Twenty-eight meristems were isolated and micrografted, and only two (7.1%) regenerated and were found to be free of GLRaV-3. The complete eradication of GLRaV-3 was achieved in several studies by thermotherapy combined with shoot apices micrografting [22], somatic embryogenesis [16,19], and cryotherapy [15]. Different efforts for GLRaV-3 elimination had thermotherapy [6,11,23] and different chemotherapeutics in combination with or not with thermotherapy [11,23].…”

Section: Discussionmentioning

confidence: 99%

“…Thermotherapy at 36-38 • C for at least 6 weeks and the isolation of smaller meristems, followed by micrografting to accelerate the regeneration process, resulted in the complete elimination of GRSPaV from all regenerated plants. Somatic embryogenesis was also very efficient in GRSPaV eradication [10,16,19,42], although Turcsan et al [10] reported that when the same procedure was applied as in the other varieties with 100% virus eradication, the highest elimination rate for the 'Sziren' variety was 54%. Different proportions of GRSPaV-free plants were obtained with chemotherapy and its combination with other methods, such as thermotherapy and shoot tip culture [12,13,24,55].…”

Section: Discussionmentioning

confidence: 99%

“…This is one of the main reasons why clonal selection is very difficult. Various measures for producing virus-and viroid-free grapevine material have been used, including thermotherapy [5,6], meristem tissue culture [7][8][9][10], micrografting, chemotherapy [11][12][13][14], cryotherapy [15], somaclonal embryogenesis [10,[16][17][18][19], electrotherapy [20], and various combinations of these treatments [21][22][23][24]. Thermotherapy in combination with a meristem tissue culture is a widely used method.…”

Section: Introductionmentioning

confidence: 99%

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Elimination of Eight Viruses and Two Viroids from Preclonal Candidates of Six Grapevine Varieties (Vitis vinifera L.) through In Vivo Thermotherapy and In Vitro Meristem Tip Micrografting

Miljanić

Rusjan

Škvarč

et al. 2022

Plants

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Viruses and virus-like organisms are a major problem in viticulture worldwide. They cannot be controlled by standard plant protection measures, and once infected, plants remain infected throughout their life; therefore, the propagation of healthy vegetative material is crucial. In vivo thermotherapy at 36–38 °C for at least six weeks, followed by meristem tip micrografting (0.1–0.2 mm) onto in vitro-growing seedling rootstocks of Vialla (Vitis labrusca × Vitis riparia), was successfully used to eliminate eight viruses (grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine Pinot gris virus (GPGV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV), grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus-1 (GSyV-1), and raspberry bushy dwarf virus (RBDV)), as well as two viroids (hop stunt viroid (HSVd) and grapevine yellow speckle viroid 1 (GYSVd-1)) from preclonal candidates of six grapevine varieties (Vitis vinifera L.). A half-strength MS medium including vitamins supplemented with 30 g/L of sucrose and solidified with 8 g/L of agar, without plant growth regulators, was used for the growth and root development of micrografts and the subsequently micropropagated plants; no callus formation, hyperhydricity, or necrosis of shoot tips was observed. Although the overall regeneration was low (higher in white than in red varieties), a 100% elimination was achieved for all eight viruses, whereas the elimination level for viroids was lower, reaching only 39.2% of HSVd-free and 42.6% GYSVd-1-free vines. To the best of our knowledge, this is the first report of GPGV, GRVFV, GSyV-1, HSVd, and GYSVd-1 elimination through combining in vivo thermotherapy and in vitro meristem tip micrografting, and the first report of RBDV elimination from grapevines. The virus-free vines were successfully acclimatized in rockwool plugs and then transferred to soil.

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“…Different methods were developed. GLRaV-3, GVA, and GRSPaV were eliminated from infected grapes using somatic embryogenesis (Bouamama Gzara et al, 2017).…”

mentioning

confidence: 99%

Sanitation of Autochthonous Grapevine Varieties from Algeria by Chemotherapy

Aiter

Lehad

Haddad

et al. 2020

Acta Phytopathologica et Entomologica Hungarica

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Several grapevine viruses were reported in Algeria and especially in grapevine germplasm collection, therefore it is a great challenge to free these varieties from virus infection before any breeding programs. Our study focused on the development of chemotherapy on autochthonous varieties collected in the grapevine germplasm collection of ITAFV. All these varieties were tested by DAS-ELISA and the presence of GLRaV-3 and GFLV was confirmed in all used samples for the sanitation. After 8 weeks of shoot tips in vitro culture in a modified M S medium containing ribavirin, DAS-ELISA test revealed that GLRaV-3 was completely eliminated and GFLV to a significant rate.

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Elimination of Grapevine leafroll associated virus-3, Grapevine rupestris stem pitting associated virus and Grapevine virus A from a Tunisian Cultivar by Somatic Embryogenesis and Characterization of the

Cited by 10 publications

References 23 publications

Grapevine virome and production of healthy plants by somatic embryogenesis

Grapevine virome and production of healthy plants by somatic embryogenesis

Elimination of Eight Viruses and Two Viroids from Preclonal Candidates of Six Grapevine Varieties (Vitis vinifera L.) through In Vivo Thermotherapy and In Vitro Meristem Tip Micrografting

Sanitation of Autochthonous Grapevine Varieties from Algeria by Chemotherapy

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