Elimination of Interfering Chromogens in the Zimmerman Reaction for Measuring 17-Ketosteroids

Our patient had a left suprarenal mass. His blood pressure was normal, but his urinary catecholamines (CA), vanillylmandelic acid (VMA), total metanephrines (TMn) and 5-hydroxyindolacetic acid (SHIAA) were elevated. In addition, he had elevated, nonsuppressible urinary 17-ketosteroids (17KS) and androsterone, but his urinary 17-hydroxycorticoids (170HCS) and free cortisol were normal, as were his plasma cortisol and ACTH. After resection of the suprarenal mass, the patient's urinary hormone values reverted to normal. The mass contained a pheochromocytoma and an adrenocortical adenoma. The pheochromocytoma was unusual in that it contained very little norepinephrine (NE) and dopamine (DA) and an abundance of epinephrine (E) despite normal enzyme concentrations. Electron micrographs showed primarily E granules with few of the NE-type. The immunoperoxidase histochemical stains for vasoactive intestinal peptide (VIP) and serotonin (S) were strongly positive. The patient's blood pressure may have been normal because his pheochromocytoma secreted E, VIP, or S. The associated adrenocortical adenoma produced no symptoms and was probably coincidental.

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Zimmermann Reaction

2010

Comprehensive Organic Name Reactions and Reagents

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The reaction between an enolate carbanion and m ‐dinitrobenzene ( m ‐DNB) has been reported by Janovsky and Erb and it is Zimmermann who initially applied the specific reaction between m ‐dinitrobenzene and 17‐ oxo ‐steroids (or 17‐ keto ‐steroids) in the presence of an alkali to the field of ketosteroid analysis. This kind of reaction is generally known as the Zimmermann reaction. Due to the specific color reaction for 17‐ oxo ‐steroids, the Zimmermann reaction has been routinely used for the various clinical testings. Besides the interference from carbonyl compounds, several limitations of this reaction have been discussed.

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Elimination of Interfering Chromogens in the Zimmerman Reaction for Measuring 17-Ketosteroids

Cited by 16 publications

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Occlusion, Topical Corticosteroids and Heat in Psoriasis

An unusual pheochromocytoma associated with an androgen secreting adrenocortical adenoma. Evaluation of its polypeptide hormone, catecholamine, and enzyme characteristics

Zimmermann Reaction

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