Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Bedwell, David W.; Rivera, Victor R.; Merrill, Gerald A.

doi:10.1006/abio.2000.4683

Cited by 16 publications

(11 citation statements)

References 10 publications

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“…In the present study, the CL spectrum of peroxynitrous acid in carbonate solution showed that there was only a peak band in the range of 416-460 nm, which resulted from the reaction HCO 3 • radicals [21,23] and these results are in good agreement with the previous studies [8,15].…”

Section: Spectrumsupporting

confidence: 81%

“…•− radical with bicarbonate leads to the generation of HCO 3 • radical [21][22][23], which is attributed to the proposed CL emitter in this study. In our experiment, an optimal amount of cotton of 0.03 g was chosen on account of larger amounts of cotton leading to blockage of FIA channels.…”

Section: Solid Materialsmentioning

confidence: 94%

“…This is because that peroxynitrite can react with carbon dioxide to form the more reactive free radical species of ONOOCO 2 − and HCO 3 • radicals, which can react with luminol and form free luminol radical more rapidly [15,16]. Furthermore, Radi and coworkers [8] observed a spontaneous CL phenomenon during the interactions of peroxynitrite with carbon dioxide/bicarbonate, suggesting the formation of HCO 3…”

Section: Introductionmentioning

confidence: 99%

“…For its measurement, many analytical methods have been reported, such as spectrophotometric [1], electrochemical [2], and fluorometric [3] methods. The commonly employed spectrophotometric methods based on the diazo coupling reaction require close control of pH and temperature during the diazotization step and a relatively long coupling time.…”

Section: Introductionmentioning

confidence: 99%

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Chemiluminescence study of carbonate and peroxynitrous acid and its application to the direct determination of nitrite based on solid surface enhancement

Lin

Huie

et al. 2004

Analytica Chimica Acta

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Peroxynitrous acid (ONOOH) was produced by the on-line mixing of acidified hydrogen peroxide with nitrite in a flow system. A strong chemiluminescent (CL) emission was observed when ONOOH reacted with carbonate without any special CL reagents. When cotton was present in the CL cell, the CL emission was enhanced significantly. The method was developed to determine nitrite, which showed a key improvement that any CL reagents and sensitizers were not used, resulting in better selectivity. The applicability of the present CL system was demonstrated for the sensitive and selective determination of nitrite in natural water samples without any special pretreatment. Good agreements were obtained for the determination of nitrite in tap and well waters between the present approach and a standard spectrophotometric method. The average precision was 4.6% (n = 7) and detection limit (S/N = 3) was 1.0 × 10 −7 M. Based on the CL spectrum, UV spectra, and dissolved oxygen measurement, a possible CL mechanism was proposed. ONOOH was an unstable compound in acidic solution and could be quenched into peroxynitrite (ONOO − ) in basic solution. ONOO − reacted with CO 2 to produce ONOOCO 2 − , which can rapidly decompose into• NO 2 and • CO 3 − radicals. In the presence of H + ,• CO 3 − radicals can protonate to bicarbonate radical (HCO 3 • ). The recombination of HCO 3• radicals and decomposition can lead to light emission.

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Section: Spectrumsupporting

confidence: 81%

Section: Solid Materialsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemiluminescence study of carbonate and peroxynitrous acid and its application to the direct determination of nitrite based on solid surface enhancement

Lin

Huie

et al. 2004

Analytica Chimica Acta

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“…Until now, many approaches for nitrite determination have been put forward, such as spectrophotometry, [5][6][7][8] ion chromatography, 9 voltammetry, 10 spectrofluorometry, 11,12 chemiluminescence, 13 and kinetic methods. 14,15 These methods have been reviewed by Moorcroft et al 16 Among them, spectrophotometric and fluorometric methods are widely used.…”

Section: Introductionmentioning

confidence: 99%

A Kinetic Spectrophotometric Method for the Determination of Nitrite by Stopped-Flow Technique

Wang

Gao

2006

ANAL. SCI.

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A novel and highly sensitive stopped-flow kinetic spectrophotometric method for the determination of nitrite, based on monitoring the variation in the absorbance of the intermediate within a very short period, has been developed. The optimum conditions for various parameters on which the reaction of nitrite with perphenazine depends, were investigated. It was found that the initial reaction rate increased linearly with increasing nitrite concentration in the range from 1.0 × 10 -8 to 6.0 × 10 -6 M. The detection limit was calculated to be 4.8 × 10 -9 M. This method was used for the determination of nitrite in natural and drinking-water with satisfactory results. The influence of cationic, non-ionic and anion surfactants was also studied in this work.

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A simple and accurate method to determine nitrite and nitrate in serum based on high‐performance liquid chromatography with fluorescence detection

Zhan

Shao

et al. 2013

Biomedical Chromatography

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A simple method for accurate determination of nitrite and nitrate in serum was proposed to avoid the variation of nitrate reduction. For nitrite determination, serum samples were directly precipitated with methanol pre-nitrate conversion, and then the supernatant reacted with 2,3-diaminonaphthalene (DAN) to form 2,3-naphthotriazole (NAT), which was quantitatively analyzed by high-performance liquid chromatography coupled with fluorescence detection (HPLC-FL). For nitrate determination, samples were firstly heated at 70°C for 10 min to inactivate endogenous reductase-inhibiting proteins, then nitrate in the samples was quantitatively reduced to nitrite by reductase added experimentally. The difference in total nitrite concentrations between pre- and post-nitrate conversion was used to calculate the amount of nitrate in the samples. In addition to good specificity, high sensitivity, satisfactory accuracy and reproducibility, our method is simple and suitable for the quantitative determination of nanomolar level of nitrite and nitrate in a large number of serum samples.

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Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Cited by 16 publications

References 10 publications

Chemiluminescence study of carbonate and peroxynitrous acid and its application to the direct determination of nitrite based on solid surface enhancement

Chemiluminescence study of carbonate and peroxynitrous acid and its application to the direct determination of nitrite based on solid surface enhancement

A Kinetic Spectrophotometric Method for the Determination of Nitrite by Stopped-Flow Technique

A simple and accurate method to determine nitrite and nitrate in serum based on high‐performance liquid chromatography with fluorescence detection

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