Elimination of multidrug-resistant Proteus mirabilis biofilms using bacteriophages

Gomaa, Salwa; Serry, Fathy; Abdel-Latif, Hemmat K.; Abbas, Hisham A.

doi:10.1007/s00705-019-04305-x

Cited by 25 publications

(19 citation statements)

References 57 publications

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“…Phage species associated with healed wounds may have profound impacts on host function, including reduction or inhibition of biofilm formation [61,62], motility inhibition equivalent to a pilus knockout [61], CRISPR resistance or inhibition [62,63], and anti-biofilm activity via capsid-displayed pectin lyase-like domains [64]. Biofilms are a leading impediment to wound healing, and exploitation of phage or their proteins as anti-biofilm agents is a very active area of research [34,[65][66][67]. Suppression of the CRISPR system might confer an advantage to the phage in evading degradation by the host.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work has determined that the human virome is mostly composed of bacteriophages, viruses that infect bacteria [27,28]. The ability of bacteriophages to infect, kill, and modulate bacterial host function has been well-described [29][30][31], and the wound virome may therefore represent a previously understudied explanatory variable for inter-patient variability, healing outcomes, community dynamics, and pathogenicity [28,[32][33][34]. Despite its potentially influential role in the bacterial microbiome, studies of the virome are often hindered by insufficient sequencing depth.…”

Section: Introductionmentioning

confidence: 99%

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The chronic wound virome: phage diversity and associations with wounds and healing outcomes

Verbanic

Deacon

Chen

2022

Preprint

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Two leading impediments to chronic wound healing are polymicrobial infection and biofilm formation. Recent studies have characterized the bacterial fraction of these microbiomes and have begun to elucidate compositional correlations to healing outcomes. However, the factors that drive compositional shifts are still being uncovered. The virome may play an important role in shaping bacterial community structure and function. Previous work on the skin virome determined that it was dominated by bacteriophages, viruses that infect bacteria. To characterize the virome, we enrolled 20 chronic wound patients presenting at an outpatient wound care clinic in a microbiome survey, collecting swab samples from healthy skin and chronic wounds before and after a single, sharp debridement procedure. We investigated the virome using a virus-like particle enrichment procedure, shotgun metagenomic sequencing, and a k-mer-based, reference-dependent taxonomic classification method. Taxonomic composition, diversity, and associations to covariates are presented. We find that the wound virome is highly diverse, with many phages targeting known pathogens, and may influence bacterial community composition and functionality in ways that impact healing outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The chronic wound virome: phage diversity and associations with wounds and healing outcomes

Verbanic

Deacon

Chen

2022

Preprint

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“…strains is increasing, necessitating a more intensive search for alternative approaches [ 57 ]. The use of therapeutic bacteriophages and their enzymes for biofilm control is a promising research direction—a number of phage cocktails have been tested in clinical trials, including a recently finished one, in which bacteriophage therapy was found to be non-inferior to the standard-of-care antibiotic treatment of UTIs [ 58 , 59 , 60 , 61 ]. The research into the application of bacteriophages against Proteus has attracted a certain amount of attention (e.g., [ 45 , 62 ]), with a recent study reporting encouraging results of the use of phage-functionalized hydrogels for the prevention of catheter blockage [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

et al. 2021

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The adherence of Proteus mirabilis to the surface of urinary catheters leads to colonization and eventual blockage of the catheter lumen by unique crystalline biofilms produced by these opportunistic pathogens, making P. mirabilis one of the leading causes of catheter-associated urinary tract infections. The Proteus biofilms reduce efficiency of antibiotic-based treatment, which in turn increases the risk of antibiotic resistance development. Bacteriophages and their enzymes have recently become investigated as alternative treatment options. In this study, a novel Proteus bacteriophage (vB_PmiS_PM-CJR) was isolated from an environmental sample and fully characterized. The phage displayed depolymerase activity and the subsequent genome analysis revealed the presence of a pectate lyase domain in its tail spike protein. The protein was heterologously expressed and purified; the ability of the purified tail spike to degrade Proteus biofilms was tested. We showed that the application of the tail spike protein was able to reduce the adherence of bacterial biofilm to plastic pegs in a MBEC (minimum biofilm eradication concentration) assay and improve the survival of Galleria mellonella larvae infected with Proteus mirabilis. Our study is the first to successfully isolate and characterize a biofilm depolymerase from a Proteus phage, demonstrating the potential of this group of enzymes in treatment of Proteus infections.

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“…Bacteriophage biofilm reduction assay was performed using a method adapted from the previously described protocol. 30 The diluted P. aeruginosa ATCC 27853 cells (150 μl) were dispensed in wells of a flat bottom microplate with a blank medium as control. After 48 h incubation, wells were washed three times with phosphatebuffered saline (PBS) buffer pH 7.4.…”

Section: Biofilm Assaymentioning

confidence: 99%

Characterization of N4-like Pseudomonas Phage vB_Pae-PA14 Isolated from Seawater Sampled in Thailand

Srichaisupakit¹,

Chopjitt²,

Kerdsin³

2021

J. Pure Appl. Microbiol.

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Bacteriophage, a predator virus of bacteria, is an abundant biological entity in the biosphere. With ultimate applications in medicine and biotechnology, new phages are extensively being isolated and characterized. The objective of the present study was to characterize lytic bacteriophage vB_Pae-PA14 infecting Pseudomonas aeruginosa ATCC 27853 that was isolated from seawater in Thailand. vB_Pae-PA14 was subjected to whole genome phylogenetic analysis, host range test, biofilm test and characterization. Results showed that the phage belonged to a group of N4-like viruses, could infect P. aeruginosa isolates including carbapenem-resistant P. aeruginosa. The burst size of vB_Pae-PA14 was 86 plaque-forming unit/infected cells. Also, the phage showed a greater ability to control planktonic P. aeruginosa cells than the biofilm cells. Phage could withstand physical stresses especially the high salt concentration. In brief, lytic bacteriophage vB_Pae-PA14 infecting P. aeruginosa was isolated and characterized, which might be useful in further bacteriophage lytic applications.

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Elimination of multidrug-resistant Proteus mirabilis biofilms using bacteriophages

Cited by 25 publications

References 57 publications

The chronic wound virome: phage diversity and associations with wounds and healing outcomes

The chronic wound virome: phage diversity and associations with wounds and healing outcomes

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

Characterization of N4-like Pseudomonas Phage vB_Pae-PA14 Isolated from Seawater Sampled in Thailand

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