Elimination of orally administered 17α-methyltestosterone by Oreochromis mossambicus (tilapia) and Salmo gairdneri (rainbow trout) juveniles

Johnstone, R.; Macintosh, D. J.; Wright, R.S.

doi:10.1016/0044-8486(83)90095-9

Cited by 60 publications

(28 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the uptake and of several natural and synthetic androgens. Investiclearance of gonadal steroids when administered at gations include those of Schreck (1973), in rainbow higher doses, as required for hormonal sex control, trout (Oncorhynchus mykiss); Fagerlund and were investigated in medaka (Oryzias latipes) McBride (1978) and Fagerlund and Dye (1979), in (Hishida 1962(Hishida , 1965, tilapia (Oreochromis mossambicus) and rainbow trout (Johnstone et al 1983), blue tilapia (Oreochromis aureus) (Goudie et al 1986a, b), Nile tilapia (Oreochromis niloticus) (Curtis et al 1988), rainbow trout (Cravedi et al 1989) and in hybrids between 0. niloticus and 0. aureus (Rothbard et al 1990). Characteristics of most of the studies cited above were the use of yearling fish and the oral route of administration.…”

Section: Introductionmentioning

confidence: 99%

Uptake and clearance of exogenous estradiol-17β and testosterone during the early development of coho salmon (Oncorhynchus kisutch), including eggs, alevins and fry

Piferrer

Donaldson

1994

Fish Physiol Biochem

View full text Add to dashboard Cite

The uptake and clearance of estradiol-17β (E2) and testosterone (T) were examined during the initial stages of development of coho salmon (Oncorhynchus kisutch), including eyed-eggs, newly hatched alevins and first feeding fry. Radiolabeled steroids were administered through the water in tracer amounts with or without their nonradioactive form at 400 μg l(-1). Regardless of developmental stage, saturation levels were invariably attained earlier for T than for E2, thus resulting in a higher incorporation of E2. However, both steroids had similar clearance patterns. Uptake and clearance was clearly stage-dependent, being fastest in fry, intermediate in alevins and slowest in eggs. Furthermore, combined uptake and clearance patterns showed that exposure to steroid was also higher for E2 than for T and stage-dependent, but always markedly highest in alevins. Subsequently, based on the observed elimination of the estrogen, a double immersion in E2 at 400 μg 1(-1), administered 2 days apart to maximize exposure during the alevin stage, was assayed for its effect on sex reversal and found to induce the production of 100% females. We suggest that the yolk, which is present in substantial amounts during the initial stages of development in salmonids, can retain the exogenously administered liposoluble steroids, thus providing developing embryos with an extended supply of, and exposure to, these steroids well after the treatment is finished. Together, these findings help to explain the previously observed high effectiveness of sex steroids administered during early development in regulating gonadal differentiation in salmonids, the higher effectiveness of E2 compared to T, and clarify the localization of the most sensitive period to the action of exogenous steroids at the alevin stage in the coho salmon.

show abstract

Section: Introductionmentioning

confidence: 99%

Uptake and clearance of exogenous estradiol-17β and testosterone during the early development of coho salmon (Oncorhynchus kisutch), including eggs, alevins and fry

Piferrer

Donaldson

1994

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…1985), infusions (Flechter et al 1969;Henderson et al 1974;Barroiller et al 1987) or on ingestion of pharmacological doses of steroids (Fagerlund and McBride 1978;Fagerlund and Dye 1979;Lone and Matty 1981;Johnston et al 1983). This later approach cannot be compared to ours since in those cases the concentration equilibrium between the various forms of plasma E, was probably modified, the steroid generally studied was an androgen, and usually the samples were collected at less regular intervals than we used.…”

Section: Discussionmentioning

confidence: 97%

Plasma kinetics of ingested tritiated estradiol and the influence on estradiol plasma levels in the cultured Siberian sturgeonAcipenser baeri

Pelissero

Menn

Narbonne

1991

Fish Physiol Biochem

View full text Add to dashboard Cite

Plasma kinetics of tritiated estradiol (E2) were studied in the Siberian sturgeonAcipenser baeri in order to explain the large amount of E2 found in plasma of 4, 5 and 10-year-old cultured males. This work presents two approaches. The first is based on a single ingestion of(3)H-labelled E2, which allowed us to plot model curves of resorption and elimination processes. The second deals with chronic ingestion of(3)H-labelled E2 during a five day period, twice a day, based on a rhythm copied from the feeding practice on a fish farm. Three different doses were tested, based on the amount present in fish diets. Oral administration of E2 to sturgeon leads to E2 accumulation and a saturation of the metabolic processes generally involved in the elimination of aromatic xenobiotics. An explanation of this progressive accumulation of E2 in sturgeon plasma has to take into account the steroid binding proteins. Their synthesis could be induced by the orally administrated E2 and protect it from metabolism.

show abstract

“…Moreover, Curtis et al (1991) reported that MT was not detected at 100 hours after immersion. Similarly, Johnstone et al (1983) reported MT level of rainbow fish plummeted until 300 hours after oral administration of MT. In the present study, the levels of testosterone in both the MT treatments and control were getting similar (2 ng/g) on day-28.…”

Section: Testosterone Levels and Functional Male Identificationmentioning

confidence: 99%

The sex ratio and testosterone levels in tilapia immersed in different doses of 17α-methyltestosterone

Zairin¹,

Naufal²,

Setiawati³

et al. 2017

Jurnal Akuakultur Indonesia

View full text Add to dashboard Cite

Nile tilapia farming using monosex male population has been reported to improve the productivity by 10% compared to that of mix-sex culture. This study aimed to determine an immersion dose of 17α-metiltestosterone (MT) that resulted in higher male percentage, growth, survival, and to measure testosterone level in the fish body. The experiment compared three MT immersion doses, namely: 0; 1.8; and 5.4 mg/L, each with three replications. Immersion was conducted to 14-days-old larvae for four hours, with a density of 100 fish/L of water. Testosterone levels in fish was measured using ELISA method, and sex identification was performed by histological method. The result showed that the percentage of male fish was the same in treatments 1.8 mg/L and 5.4 mg/L, which were 53-65% higher than that of the control without MT treatment. Fish growth and survival until day 56 post immersion were not significantly different amongst treatments. The results of ELISA analysis showed that the levels of testosterone in larvae just after immersion was similar in 1.8 mg/L and 5.4 mg/L treatments, which then drastically decreased on day 14 after immersion. On day 28 after immersion the levels of testosterone of the MT treatments were similar to that of the control, i.e. about 2 ng/g. On day 71 the testosterone levels in sex reversed and normal males fish remained the same at about 0.3 ng/g (P>0.05). By PCR method with specific primer, sex reversed and normal males could be distinguished. In conclusion, sex reversal by immersion at a dose of 1.8 mg/L can be considered as a standard protocol for Nile tilapia sex reversal. Testosterone level in the body of MT-treated fish could be reduced to the same level as the control in less than one month post immersion.Keywords: sex reversal, 17α-methyltestosterone, testosterone levels, immersion, tilapia ABSTRAK Budidaya ikan nila menggunakan sistem monoseks jantan telah dilaporkan dapat meningkatkan produktivitas hingga 10%. Penelitian ini dilakukan menentukan dosis perendaman hormon 17α-metiltestosteron (MT) yang menghasilkan rasio ikan jantan tertinggi, pertumbuhan, sintasan, dan mengukur kadar testosteron dalam tubuh ikan nila. Dosis hormon MT yang diuji adalah 0; 1,8; dan 5,4 mg/L. Setiap perlakuan dosis diberi tiga ulangan. Perendaman dilakukan selama empat jam, dengan jumlah larva (umur 14 hari pascatetas) 100 ekor dalam 1 L air. Hasil analisis histologi menunjukkan bahwa persentase ikan nila jantan adalah sama pada perlakuan 1,8 mg/L dan 5,4 mg/L, dan 53-65% lebih tinggi daripada kontrol tanpa direndam MT (54% jantan). Dengan analisis ELISA, kadar testosteron dalam tubuh larva sesaat setelah perendaman MT dosis 1,8 mg/L dan 5,4 mg/L adalah sama, tetapi turun drastis pada hari ke-14 pascarendam, kemudian kadarnya menjadi relatif sama pada hari ke-28, yaitu 2 ng/g). Pertumbuhan bobot dan sintasan ikan sampai hari ke-56 adalah sama. Dengan menggunakan metode PCR, jantan fungsional dan jantan normal dapat dibedakan, dan pada hari ke-71 kadar testosteron ikan jantan fungsional dan jantan...

show abstract

Elimination of orally administered 17α-methyltestosterone by Oreochromis mossambicus (tilapia) and Salmo gairdneri (rainbow trout) juveniles

Cited by 60 publications

References 11 publications

Uptake and clearance of exogenous estradiol-17β and testosterone during the early development of coho salmon (Oncorhynchus kisutch), including eggs, alevins and fry

Uptake and clearance of exogenous estradiol-17β and testosterone during the early development of coho salmon (Oncorhynchus kisutch), including eggs, alevins and fry

Plasma kinetics of ingested tritiated estradiol and the influence on estradiol plasma levels in the cultured Siberian sturgeonAcipenser baeri

The sex ratio and testosterone levels in tilapia immersed in different doses of 17α-methyltestosterone

Contact Info

Product

Resources

About