Elimination of pathogenic multidrug resistant isolates through different metal oxide nanoparticles synthesized from organic plant and microbial sources

Alsamman, Alhadary M.; Khedr, M.H.; Kabary, Hoda; El-Sehrawy, M.H.

doi:10.1016/j.micpath.2023.106055

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…A complete chemical analysis of plant extract was completed after extract preparation, as described by Alsamman et al [ 33 ]. The detection tests for each component, such as carbohydrates, fixed oils, fats, phenol, and tannins, were conducted.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, Loo et al [ 131 ] observed that AgO NPs minimum inhibitory concentration (MIC) against Salmonella typhimurium , Escherichia coli , Klebsiella pneumoniae , and Salmonella enteritidis was 7.8, 3.9, 3.9, and 3.9 μg/mL, in that order. AgO NPs’ bactericidal activity has been evaluated using growth curves at doses ranging from 0 to 8×MIC dilution factor [ 33 ]. Khaydarov et al [ 132 ] and Yousef et al [ 133 ] reported that the AgNPs MIC values for S. aurues and Enterococcus faecalis were 3 and 2 mg/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Khaydarov et al [ 132 ] and Yousef et al [ 133 ] reported that the AgNPs MIC values for S. aurues and Enterococcus faecalis were 3 and 2 mg/mL, respectively. Alsamman et al [ 33 ] reported that AgO NPs exhibit maximum antibacterial activity among the selected five MDR-tested isolates, with TiO 2 nanoparticles being the second most effective type. Microbial AgO had an average size of 30 nm by TEM with a smooth and regular spherical shape, while plant-mediated AgO NPs had an average size of 52 nm as demonstrated by TEM examination.…”

Section: Discussionmentioning

confidence: 99%

“…Microbial AgO had an average size of 30 nm by TEM with a smooth and regular spherical shape, while plant-mediated AgO NPs had an average size of 52 nm as demonstrated by TEM examination. AgO and TiO 2 showed notable antibacterial activity for all the MDR isolates tested, especially against E. coli and Staphylococcus sciuri [ 33 ]. The findings, determined by Sharifi-Rad et al [ 120 ], demonstrate the antibacterial activity of the A. tribuloides root extract and the greenly synthesized AgNPs against the Gram-positive ( B. cereus and S. aureus ) and Gram-negative bacterial strains ( E. coli and Sh.…”

Section: Discussionmentioning

confidence: 99%

“…1. The residual water was removed through freeze-drying and stored at 4 °C, while the methanol was subsequently evaporated using a rotary [ 33 ]. The protocol of Oves et al [ 34 ] was followed with some modifications.…”

Section: Methodsmentioning

confidence: 99%

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Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Mekky,

Abdelaziz,

Youssef

et al. 2024

Medicina

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Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs.

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Section: Resultsmentioning

confidence: 99%