Elimination of plasma lipids from steroid hormone extracts by differential solubility in water

Ismail, A. A. A.; Love, D. N.; McKinney, R. W.

doi:10.1016/s0039-128x(72)80034-5

Cited by 9 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The wavelengths may need to be varied slightly for other steroids. 3 Chart recorder (Bryan model No 24000 A3, VA Howes, London) was used. 4 Glass plates for thin layer chromatography measured 20 x 5 x 0-3 cm.…”

Section: Methodsmentioning

confidence: 99%

Fluorimetric method for simultaneous estimation of cortisol, corticosterone, and testosterone in plasma.

Mattingly

Martin

Tyler

1989

Journal of Clinical Pathology

View full text Add to dashboard Cite

SUMMARY The simultaneous estimation of steroids in plasma was carried out by the assay of cortisol, corticosterone, and testosterone. The method entails separation by means of thin layer chromatography, followed by conversion to a fluorophore and fluorimetric measurement. Its major advantages are its high specificity, its ability to detect unknown substances, and the ease with which it can be performed. The method has acceptable levels of accuracy and precision and the normal values obtained by it compare well with those given by methods in general use.

show abstract

Section: Methodsmentioning

confidence: 99%

Fluorimetric method for simultaneous estimation of cortisol, corticosterone, and testosterone in plasma.

Mattingly

Martin

Tyler

1989

Journal of Clinical Pathology

View full text Add to dashboard Cite

show abstract

“…This was followed by an alkali wash with 1 M-NaOH and neutral and nonpolar lipids were removed by differential solubility in 1 % CaCl2 (Ismail, Love & McKinney, 1972).…”

Section: Animalsmentioning

confidence: 99%

Tissue Androgen Concentrations in Golden Hamsters With Benign Prostatic Tumours

Finney¹,

Harper²,

Gaskell³

et al. 1980

Journal of Endocrinology

View full text Add to dashboard Cite

Using gas chromatography-high resolution mass spectrometry with selected ion detection, the concentrations of testosterone, 5alpha-dihydrotestosterone, androsterone, 5alpha-androstane-3alpha,17beta-diol, 5alpha-androstane-3beta,17alpha-diol and 5alpha-androstane-3beta,17beta-diol were measured in the dorsal and ventral prostates of two strains of golden hamster (Mesocricetus auratus) at various ages from 60 to 250 days. The tissue concentrations from a control strain were compared with those of the BIO 87.2 strain of hamster, the latter developing benign hyperplasia of the prostate between 90 and 120 days of age. Marked increases in the concentration of total 5alpha-androstanediols in both prostatic lobes of the BIO 87.2 strain were detected, with the highest level of over 345 nmol/g protein being found at 200 days of age. In comparison, the control strain showed a less pronounced increase in concentration at 150 days. Increases in total 5alpha-androstanediols were mainly associated with increases in the concentration of 5alpha-androstane-3alpha,17beta-diol. Concentrations of testosterone in prostatic tissue were also found to increase in the BIO 87.2 animals with peak values being seen at 200 days. Increases in concentration of androsterone were observed by 150 days and these levels were maintained up to 250 days of age. Control animals showed no comparable increases in testosterone and androsterone during the time-course studied. Surprisingly, no significant variation in 5alpha-dihydrotestosterone concentration was detected in either strain from 60 to 250 days although levels were slightly but consistently higher in the BIO 87.2 strain. There were no significant fluctuations in any of the androgens assayed either before or during tumour development. The increases in 5alpha-androstane-3alpha,17beta-diol, testosterone and androsterone were detected only after tumours had become established.

show abstract

Thin-layer chromatography and high resolution selected ion monitoring for the analysis of C19 steroids in human hyperplastic prostate tissue

Millington

Buoy

Brooks

et al. 1975

Biol. Mass Spectrom.

View full text Add to dashboard Cite

Abstract-Interfering nonpolar lipid material was removed from the acetone extracts of several samples of benign hyperplastic prostate tissue. Endogenous steroids were separated by preparative thin-layer chromatography into fractions contahing testosterone, Sa-dihydrotestosterone and the 5a-androstane-3,17-diols, and concentrations of these steroids were measured by molecular ion monitoring of suitable derivatives at a resolution of 8500 to 10 OOO during combined gas chromatography mass spectrometry. The sensitivity and specificity of this procedure allowed unequivocal detection of the steroids of interest at the femtomole level and the incorporation of epimers as internal standards enabled accurate quantitative measurements. The results indicated that 5a-dihydrotestosterone is usually the most predominant of the steroids measured, and that of the 5a-androstanediols, only the 3a, 17p and 3P,17p isomers occur in measurable concentration, with the former usually predominant.

show abstract

Elimination of plasma lipids from steroid hormone extracts by differential solubility in water

Cited by 9 publications

References 12 publications

Fluorimetric method for simultaneous estimation of cortisol, corticosterone, and testosterone in plasma.

Fluorimetric method for simultaneous estimation of cortisol, corticosterone, and testosterone in plasma.

Tissue Androgen Concentrations in Golden Hamsters With Benign Prostatic Tumours

Thin-layer chromatography and high resolution selected ion monitoring for the analysis of C19 steroids in human hyperplastic prostate tissue

Contact Info

Product

Resources

About