Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

Liu, Xiaobo; Tang, Bo; Gu, Qun; Yu, Xiaobin

doi:10.1016/j.mex.2014.08.008

Cited by 32 publications

(10 citation statements)

References 3 publications

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“…Once formed, biofilms can be extremely difficult to remove, leading to contamination of processing equipment (Kumar and Anand, 1998; Tan et al, 2014), food spoilage and consequent economic losses (Brooks and Flint, 2008; Houdt and Michiels, 2010). Numerous studies have described the use of novel approaches to control biofilms; these have included the use of biocides (Wood et al, 1996), application of electrical currents (Poortinga et al, 2001), temperature step changes during growth regions (Knight et al, 2004), enzymes (Liu et al, 2014) and for biofilms on or within a food matrix, the use of novel food additives (Furukawa et al, 2010). …”

Section: Introductionmentioning

confidence: 99%

A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes

et al. 2016

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The burden of foodborne disease has large economic and social consequences worldwide. Despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. Biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. In parallel, the growing concern of consumers regarding the use of chemically derived antimicrobials within food has led to a drive toward more natural products. As a consequence, the use of naturally derived antimicrobials has become of particular interest. In this study we investigated the efficacy of nisin A and its bioengineered derivative M21A in combination with food grade additives to treat biofilms of a representative foodborne disease isolate of Listeria monocytogenes. Investigations revealed the enhanced antimicrobial effects, in liquid culture, of M21A in combination with citric acid or cinnamaldehyde over its wild type nisin A counterpart. Subsequently, an investigation was conducted into the effects of these combinations on an established biofilm of the same strain. Nisin M21A (0.1 μg/ml) alone or in combination with cinnamaldehyde (35 μg/ml) or citric acid (175 μg/ml) performed significantly better than combinations involving nisin A. All combinations of M21A with either citric acid or cinnamaldehyde eradicated the L. monocytogenes biofilm (in relation to a non-biofilm control). We conclude that M21A in combination with available food additives could further enhance the antimicrobial treatment of biofilms within the food industry, simply by substituting nisin A with M21A in current commercial products such as Nisaplin® (Danisco, DuPont).

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Section: Introductionmentioning

confidence: 99%

A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes

et al. 2016

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“…Disruptive enzymes, such as proteases, glycosidases, amylases, cellulases, or DNAses, are considered a green alternative to chemical treatments often used in the fight against biofilms' formation in food-related environments [2]. Such enzymes do not have toxic effects and are used both alone and as part of the industrial detergents' composition to improve their cleaning efficacy [64][65][66].…”

Section: Disruptive Enzymes For Fighting Against Biofilmsmentioning

confidence: 99%

Biofilms Formed by Pathogens in Food and Food Processing Environments

Grigore‐Gurgu¹,

Bucur²,

Borda³

et al. 2020

Bacterial Biofilms

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This chapter presents the ability of some pathogenic (Listeria monocytogenes, Escherichia coli, Salmonella enterica, Campylobacter jejuni, Pseudomonas aeruginosa) and toxigenic bacteria (Bacillus cereus, Staphylococcus aureus) to form biofilms and contribute to the persistence of these microorganisms in the food industry. Particularities regarding attachment and composition of biofilms formed in food and food processing environments are presented and genes involved in biofilm production are mentioned. To give a perspective on how to fight against biofilms with new means, nonconventional methods based on bacteriocins, bacteriophages, disruptive enzymes, essential oils, nanoemulsions and nanoparticles, and use of alternative technologies (cold plasma, ultrasounds, light-assisted technologies, pulsed electric field, and high pressure processing) are shortly described.

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“…Traditional cleaning agents are also known to be ineffective and do not clean or sterilize microbes located in the inner layers of the biofilm [8]. A recent study has identified a class of fluids known as enzyme fluids [9][10] to be effective for removing biofilms in pipes. These enzyme fluids are specifically being highlighted here, as these fluids are organic and thus will be safe for human use.…”

Section: Introductionmentioning

confidence: 99%

Curtailing COVID-19 spread in drain pipelines: Using interfacial hydrodynamics for removing Bacterial and Viral Biofilms

Shahabaz¹,

Murallidharan²

2020

Preprint

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Drainage systems contains biological contaminants like bacteria and viruses flowing through them. Additionally, these pipelines also have organic matter known as biofilms growing on their walls. These biofilms infact act as incubation zones for further growth of bacteria and coronaviruses. Standard water treatment routines with traditional cleaning agents are known to be not be able to clean or sterilize microbes located in the inner layers of the biofilm. A recent study has identified specialised fluids which are effective in removing biofilms but these need to be used prudently. The present study proposes to use ‘ interfacial hydrodynamics’ to ensure that the cleaner-fluid (CF) is transported effectively to the location of the biofilms at the pipe walls, and allowed to be in contact with the biofilms for a sufficient amount of time so as to ensure its effective removal. The present study has used CFD technique of Multi-fluid VOF and has demonstrated that relative superficial velocities of cleaner- fluids and sewage water can be controlled, so as to achieve flow regimes that ensure delivery of cleaner fluid to the periphery of the tube walls. Our simulations indicate that most effective cleaning can be achieved by using a cleaner-fluid with a high viscosity ( ~5000 cP)). In such cases, a low- medium velocity (~0.05-0.3 m/s) of CF and water would ensure that the cleaner fluids are in constant contact with the pipe walls. Other suitable viscosity and velocity combinations have also been proposed. Flow parameters that can be used to monitor and cross-verify expected flow patterns on-site have also been proposed.

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Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

Abstract: Graphical abstract

Cited by 32 publications

References 3 publications

A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes

A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes

Biofilms Formed by Pathogens in Food and Food Processing Environments

Curtailing COVID-19 spread in drain pipelines: Using interfacial hydrodynamics for removing Bacterial and Viral Biofilms

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