ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Abstract

The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogen and potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodies and 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbent assay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonal antibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could be distinguished from any concentration of another gram-negative bacterium: Escherichia coli.

The Sars-Cov2 Epoch and Proper Managing Strategies to Face the Challenges Both in Viral Research and Treatment

“…In laboratory allowed condition work with living organisms, polyclonal antibody production represents the simplest way in comparison to other more sophisticated ways like monoclonal approach [65]. Since our work bases and will be based in the future in SARS-COV2 pathogens derived antigen, there will be no need to worry about to add adjuvants to avoid hypersensitive reaction or response or development of tolerability.…”