ELISA measurements of vasotocin and isotocin in plasma and pituitary of the rainbow trout: Effect of salinity

Pierson, Patricia; Guibbolini, Marielle; Mayer-Gostan, N.; Lahlou, B.

doi:10.1016/0196-9781(95)00058-r

Cited by 39 publications

(19 citation statements)

References 23 publications

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“…Early radioimmunoassay studies, using a fish-specific antibody, found no difference in plasma AVT concentrations in seawater versus freshwater acclimated European flounder, rainbow trout, and European eel (22,792), and an ELISA protocol confirmed this finding for the rainbow trout (608). In contrast, other studies determined that plasma AVT concentrations increased when the European flounder or rainbow trout was acclimated to fresh water (22,374), and a recent study showed a direct correlation between plasma AVT levels and salinity in the European flounder (53).…”

Section: Arginine Vasotocinmentioning

confidence: 57%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,325

1,788

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The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes. Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates. Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself. The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system. Nevertheless, substantial questions about the evolution of these mechanisms and control remain.

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Section: Arginine Vasotocinmentioning

confidence: 57%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,325

1,788

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“…The concentrations used in our work are within the range of the circulating peptide levels reported in fish (Henderson et al 1986, Perrott et al 1991, Warne et al 1994, Pierson et al 1995. As for the gills of SW-adapted rainbow trout, Guibbolini & Lahlou (1987) have shown that AVT and IT produced a maximal inhibition of membrane adenylate cyclase activity at 10 11 to 10 10 M. Sainsbury & Balment (1991) confirmed, on the same material, a similar maximal inhibitory effect of AVT at 10 10 to 10 9 M on cellular cAMP content.…”

Section: Avt and Itmentioning

confidence: 57%

Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Guibbolini¹,

Avella²

2003

Journal of Endocrinology

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Neurohypophysial hormone receptors were studied in primary cultures of sea bass (Dicentrarchus labrax) gill respiratory-like cells grown on permeable supports. This preparation was previously shown to provide a functional model for investigating the hormonal regulation of Cl secretion.Under control conditions, the cultured monolayered epithelium had a short-circuit current (I SC ) of 3·5 1·1 µA cm 2 . This current had previously been identified as an active Cl secretion. The addition of increasing concentrations of the fish neurohypophysial hormones, arginine vasotocin (AVT) or isotocin (IT), elicited a concentration-dependent stimulation of the I SC . Maximal increases of 60·9 12·1% and 117·7 28·0% above the basal I SC value were obtained for 10 7 M AVT and IT respectively. Half-maximal effects were obtained for 3·1 10 9 M AVT and for 1·4 10 9 M IT. Mucosal application of 1·0 mM diphenylalamine-2-carboxylic acid (a specific blocker of Cl channels) after serosal addition of 5 10 8 M AVT or IT inhibited not only the basal but also the stimulated current, revealing a correlation with a hormone-dependent Cl transport.Specific V1 or V2 receptor analogues of vasopressin (mammalian hormone) were used to characterize the type of neurohypophysial hormone receptors pharmaco- ]-vasopressin used at the same concentration had no significant effect. In contrast, similar experiments carried out in the presence of 10 8 M IT showed that both antagonists significantly reduced the IT-stimulated Cl secretion, with the efficiency of the V1 receptor antagonist being significantly greater than that of the V2.This study provides evidence for neurohypophysial hormone control of Cl secretion in fish cultured gill respiratory cells. It suggests that on a physiological basis the hormonal effect is shared by the two peptides present in fish neurohypophysis (AVT and IT), acting by means of two distinct, although pharmacologically similar, V1-type receptors (according to the mammalian classification). These specific receptors are expected to play an important role in controlling ion homeostasis in seawater fish.

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“…Vasotocin is implicated in the control of salt and water balance and blood volume regulation and has been shown to have direct vascular actions Balment, 1995, 1997). Both AVT and IT exert, depending on the dose, diuretic and antidiuretic actions in the kidney of salmonids (Pierson et al, 1995), although only antidiuretic effects may be physiologically relevant. In eel and rainbow trout (C. Teitsma and O. Kah, unpublished observations), it is known that part of the CRF-expressing neurons in the NPOmc is also AVT positive.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of glucocorticoid receptors in the forebrain of the rainbow trout (Oncorhynchus mykiss)

et al. 1998

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The distribution of glucocorticoid receptor-expressing cells was studied in the forebrain of the rainbow trout by means of antibodies produced against a fusion protein made of the NH2-terminal fragment of the rainbow trout glucocorticoid receptor fused in frame with glutathione-S-transferase. The results indicate that glucocorticoid receptor-expressing cells are located in many brain regions from the telencephalon to the spinal cord, with the highest density in the neuroendocrine component of the brain, the preoptic region and the mediobasal hypothalamus, and in the periventricular zone of the optic tectum. In virtually all cases, the labeling was located in the nucleus of the cells, although on very rare occasions, a slight labeling of the cytoplasm was detected. Concerning the preoptic region, the most striking feature was the high density of glucocorticoid receptors in the magnocellular preoptic nucleus, known to contain corticotrophin-releasing factor (CRF)-, vasotocin-, and isotocin-expressing cells. Colocalization experiments showed that 100% of the CRF-immunoreactive neurons in the preoptic nucleus express glucocorticoid receptors. In the mediobasal hypothalamus, the highest expression was found in the nucleus lateralis tuberis and parts of the nucleus recessus lateralis. Concerning the pituitary, the glucocorticoid receptor was consistently found in the rostral pars distalis, with the exception of the prolactin cells, and in the proximal pars distalis, which in trout contains thyrotrophs, gonadotrophs, and somatotrophs. In the hindbrain, expression of glucocorticoid receptors were localized mainly in the periventricular regions.

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ELISA measurements of vasotocin and isotocin in plasma and pituitary of the rainbow trout: Effect of salinity

Cited by 39 publications

References 23 publications

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Immunohistochemical localization of glucocorticoid receptors in the forebrain of the rainbow trout (Oncorhynchus mykiss)

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