1995
DOI: 10.1007/bf01886876
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ELISA quantitation of apolipoproteins in plasma lipoprotein fractions: ApoE in apoB-containing lipoproteins (Lp B:E) and apoB in apoE-containing lipoproteins (Lp E:B)

Abstract: Growing clinical evidence suggests that metabolic behavior and atherogenic potential vary within lipoprotein subclasses that can be defined by apolipoprotein variation. Variant constituency of apolipoproteins B and E (apoB and apoE) may be particularly important because of the central roles of these apolipoproteins in the endogenous lipid delivery cascade. ApoB is the sole protein of low-density lipoprotein (LDL), and like LDL cholesterol, the plasma apoB level has been positively correlated with risk for athe… Show more

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Cited by 4 publications
(3 citation statements)
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“…ApoAI and ApoB concentrations were measured by using commercially available immunoturbidimetric assay kits (Sigma Chemical Co., St. Louis, MO, USA) [16]. ApoCIII and ApoE concentrations were determined by using an ELISA method developed in our laboratory [18]. …”
Section: Methodsmentioning
confidence: 99%
“…ApoAI and ApoB concentrations were measured by using commercially available immunoturbidimetric assay kits (Sigma Chemical Co., St. Louis, MO, USA) [16]. ApoCIII and ApoE concentrations were determined by using an ELISA method developed in our laboratory [18]. …”
Section: Methodsmentioning
confidence: 99%
“…In the former case, 2 site-differential enzyme-linked immunosorbent assays (ELISAs) have been used to measure the amount of apoB associated with plasma lipoproteins containing apoE. 80,81 In normolipidemic subjects, Ϸ20% of total plasma apoB is associated with apoE, decreasing to Ϸ15% in hypercholesterolemic subjects and increasing to as much as 30% in hypertriglyceridemic and 85% in type III hyperlipoproteinemic subjects. Higher levels of apoB associated with apoE (LpE:B) occur in VLDL than in IDL or LDL, and hypertriglyceridemic subjects have LpE:B levels 2-fold higher than control subjects.…”
Section: Quantification Of Remnant Lipoproteins According To Apolipopmentioning
confidence: 99%
“…83 Two-site ELISAs have similarly been used to measure apoE in apoB-containing lipoproteins (LpB:E). 81,84 ApoE has also been measured in TRL fractions isolated by ultracentrifugation, [85][86][87][88] gel filtration chromatography, 89 -93 or plasma precipitation. 94,95 ApoE has been determined in remnant-like intermediate-sized lipoproteins (ISL) separated by gel filtration chromatography.…”
Section: Quantification Of Remnant Lipoproteins According To Apolipopmentioning
confidence: 99%