2002
DOI: 10.1016/s0022-1759(02)00274-0
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ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques

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Cited by 31 publications
(22 citation statements)
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“…These data demonstrate that it would be important to evaluate vaccine candidates for their ability to induce potent and long-lived immune responses in different species and, in particular, in nonhuman primates. In fact, rhesus macaques are a suitable model for preclinical immunogenicity studies because they are phylogenetically and physiologically similar to humans (32) and their well-characterized immune system is comparable to that of humans in terms of cytokine production and regulation (24). Both MRKAd5-NSmut and MRKAd6-NSmut vectors showed equivalent immunological potency in mice at two different doses (10 7 and 10 9 VP/dose).…”
Section: Discussionmentioning
confidence: 99%
“…These data demonstrate that it would be important to evaluate vaccine candidates for their ability to induce potent and long-lived immune responses in different species and, in particular, in nonhuman primates. In fact, rhesus macaques are a suitable model for preclinical immunogenicity studies because they are phylogenetically and physiologically similar to humans (32) and their well-characterized immune system is comparable to that of humans in terms of cytokine production and regulation (24). Both MRKAd5-NSmut and MRKAd6-NSmut vectors showed equivalent immunological potency in mice at two different doses (10 7 and 10 9 VP/dose).…”
Section: Discussionmentioning
confidence: 99%
“…We analyzed 61 peripheral lymphocyte samples taken at one to four time points per patient with IFN-γ enzyme-linked immunospot (ELISpot) assay and 101 samples (taken at four to nine time points per patient) with T cell pro- (9). Cryopreserved PBMC was thawed and incubated overnight before being added to the 10 µg/ml anti-IFN-γ mAb (Mabtech, Nacka, Sweden)-coated plates.…”
mentioning
confidence: 99%
“…Finally, the application of ELIspot and cytokine flow cytometry assays has provided new insights into the attributes of both CD4 + and CD8 + T cells that mediate protection in macaques (Mäkitalo et al 2002, Keeney et al 2003. This technology should help to identify correlates of protection in future primate vaccine studies.…”
Section: Determining Correlates Of Immune Protection To Leishmaniamentioning
confidence: 99%