Ellagic acid inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and prevents atheroma formation in streptozotocin-induced diabetic rats

Rani, P.; Kesavan, R.; Ganugula, Raghu; Avaneesh, T; Kumar, Parveen; Reddy, G. Bhanuprakash; Dixit, Madhulika

doi:10.1016/j.jnutbio.2013.04.004

Cited by 46 publications

(33 citation statements)

References 66 publications

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“…Immunohistochemistry analysis on cross sections of aorta showed reduction in medial layer thickness in vessel wall in the rats treated with streptozotocin and ellagic acid (2% in the diet), compared to the group treated with streptozotocin alone. This experimental model provided evidence of the inhibitory effect of ellagic acid on lipid accumulation and collagen deposition in the aorta [57].…”

Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 75%

“…Differently from the aforementioned studies conducted on experimental model of restenosis, the ellagic acid, a phenolic constituent present in fruits and nuts with the highest levels found in raspberries, has been tested in a diabetes-induced atherosclerotic disease [57]. Ellagic acid significantly affected PDGFR-β phosphorylation and the reactive oxygen species formation in SMCs.…”

Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 99%

“…Interestingly, the authors provided data on a docking study and found two putative interacting sites of ellagic acid with PDGF-BB-PDGFR-β complex. A first analysis of the expression levels of cell cycle proteins demonstrated a significant effect of ellagic acid on cyclin D1 expression and retinoblastoma hyperphosphorylation [57]. Immunohistochemistry analysis on cross sections of aorta showed reduction in medial layer thickness in vessel wall in the rats treated with streptozotocin and ellagic acid (2% in the diet), compared to the group treated with streptozotocin alone.…”

Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 99%

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Naturally occurring PDGF receptor inhibitors with potential anti-atherosclerotic properties

Ricci¹,

Ferri²

2015

Vascular Pharmacology

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Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 75%

Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 99%

Section: Naturally Occurring Inhibitors Of Platelet Derived Growth Famentioning

confidence: 99%

See 1 more Smart Citation

Naturally occurring PDGF receptor inhibitors with potential anti-atherosclerotic properties

Ricci¹,

Ferri²

2015

Vascular Pharmacology

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“…EA has been suggested to possess anti-inflammatory effects that reduce vascular events [37]. Cyclooxygenase 2 (COX-2) is an inflammatory marker that is upregulated in diabetic vasculature.…”

Section: Ellagic Acid Reduces Hg-induced Cox-2 Expressionmentioning

confidence: 99%

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Rozentsvit

Vinokur

Samuel

et al. 2017

Cell Physiol Biochem

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Background/Aims: Elevated production of reactive oxygen species (ROS) is linked to endothelial dysfunction and is one of the key contributors to the pathogenesis of diabetic vascular complications. Emerging evidence has indicated that ellagic acid (EA), a polyphenol found in fruits and nuts, possesses numerous biological activities including radical scavenging. However, whether EA exerts a vasculo-protective effect via antioxidant mechanisms in blood vessels exposed to diabetic conditions remains unknown. Accordingly, the goal of this current study was to determine whether EA decreases vascular ROS production and thus ameliorates endothelial dysfunction in the diabetic milieu. Methods: Intact rat aortas and human aortic endothelial cells (HAEC) were stimulated with 30mM high glucose (HG) with and without EA co-treatment. Endothelium-dependent vasodilation was measured using a wire myograph. Gene and protein expression of non-phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidases 4 (NOX4) were detected using RT-PCR and western blotting, respectively. Oxidative stress was determined by measuring ROS levels using dihydroethidium (DHE) staining. Results: Intact aortas exposed to HG condition displayed exacerbated ROS production and impairment of endothelium-dependent vasodilation, characterizing endothelial dysfunction. These effects were markedly reduced with EA treatment. HG enhanced ROS production in HAEC, paralleled by increased ERK1/2 activation and NOX4 expression. EA treatment blunted the increase of ROS generation, ERK1/2 activation and decreased NOX4. Conclusions: EA significantly decreases endothelial ROS levels and ameliorates the impairment of vascular relaxation induced by HG. Our results suggest that EA exerts a vasculo-protective effect under diabetic conditions via an antioxidant effect that involves inhibition of ERK1/2 and downregulation of NOX4.

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“…The viability of four different human cell lines (Table 3) was monitored upon treatment with different concentrations of the standard sample of CPT (>90% purity, Sigma Aldrich, Mumbai), and the crude CPT extract from the biomass. The assay protocol was adapted from Ishizawa et al (2005) and Rani et al (2013). Cell lines were procured from American Type Culture Collection (ATCC) and sub-passaged and cryopreserved as per the ATCC guidelines.…”

Section: Cytotoxicity Analysis Of the Crude Cpt Extract Obtained Frommentioning

confidence: 99%

Reprint of: Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani

Venugopalan

Potunuru

Dixit

et al. 2016

Bioresource Technology

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Volumetric productivity of camptothecin from the suspension culture of the endophyte Fusarium solani was enhanced up to ∼152 fold (from 0.19μgl(-1)d(-1) to 28.9μgl(-1)d(-1)) under optimized fermentation conditions including initial pH (6.0), temperature (32°C) and agitation speed (80rpm) with (5% (v/v)) ethanol as medium component. Among various elicitors and precursors studied, tryptamine (0.5mM) as precursor and bovine serum albumin (BSA) (0.075mM) as an elicitor added on day 6 of the cultivation period resulted in maximum enhancement of camptothecin concentration (up to 4.5 and 3.4-fold, respectively). These leads provide immense scope for further enhancement in camptothecin productivity at bioreactor level. The cytotoxicity analysis of the crude camptothecin extract from the fungal biomass revealed its high effectiveness against colon and mammary gland cancer cell lines.

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Ellagic acid inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and prevents atheroma formation in streptozotocin-induced diabetic rats

Cited by 46 publications

References 66 publications

Naturally occurring PDGF receptor inhibitors with potential anti-atherosclerotic properties

Naturally occurring PDGF receptor inhibitors with potential anti-atherosclerotic properties

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Reprint of: Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani

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