Ellagic Acid Inhibits TGFβ1/Smad-Induced Renal Fibrosis in Diabetic Kidney Injury

Saribas, Gulistan Sanem; YILDIZ>, Halime TOZAK; GORGULU, Ozkan

doi:10.18678/dtfd.1198021

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…Flavonoids are major metabolites with multiple physiological and biological processes and mainly act as antioxidants to prevent ROS-induced damage under oxidative stress conditions (17)(18)(19). Various documents have indicated the valuable effects of flavonoids in treating diabetes and its complications (20)(21)(22). Crocus sativus L. has been known for its various medical properties.…”

Section: Introductionmentioning

confidence: 99%

Crocin Protects Mice Pancreatic Islets from Oxidative Stress Induced by Methylglyoxal and Increases Insulin Secretion

RADMEHR,

AHANGARPOUR,

HAROONI

et al. 2023

Düzce Tıp Fakültesi Dergisi

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Aim: Islets of Langerhans are more sensitive to oxidative damage because of their low antioxidant capacity. In diabetes, methylglyoxal (MG) accumulates in the pancreas. The present study examined the effect of crocin on oxidative stress induced by MG in isolated Langerhans islets from male mice. Material and Methods: Twenty-four male mice weighing 20 to 25 g were prepared. The isolated Langerhans islets were transferred to the culture medium. Oxidative stress was induced through MG administration for 30 min, and then 10, 20, 30, and 40 μM of crocin was used for 2 h. Samples were divided into seven groups with 2.8, 5.6, and 16.7 mM glucose concentrations: control, MG 300 μM, MG+glibenclamide 10 μM, and MG+crocin in four doses of 10, 20, 30, and 40 μM. At the end, the islet’s insulin, antioxidant levels, and lipid peroxidation were assessed by ELISA and calorimetry methods. Results: Increased levels of malondialdehyde (MDA) in MG groups significantly decreased in 2.8 (p=0.008), 5.6 (p=0.004), and 16.7 (p˂0.001) mM glucose concentrations, with administration of 30 and 40 μM crocin. Total antioxidant capacity (TAC) was reduced in MG groups (p˂0.001) and significantly restored in all crocin-treated groups in 2.8, 5.6, and 16.7 mM glucose concentrations. Also, a significant decrease in insulin secretion and content was observed in MG groups of all three glucose concentrations (p˂0.001). Crocin at high doses improved these alterations. Conclusion: MG caused oxidative damage and reduced insulin secretion in isolated islets. Crocin improved the antioxidant defense system, diminished MDA, and increased insulin secretion.

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