Ellagic acid mitigates arsenic‐trioxide‐induced mitochondrial dysfunction and cytotoxicity in SH‐SY5Y cells

Abstract

In the current study, neuroprotective significance of ellagic acid (EA, a polyohenol) was explored by primarily studying its antioxidant and antiapoptotic potential against arsenic trioxide (As O )-induced toxicity in SH-SY5Y human neuroblastoma cell lines. The mitigatory effects of EA with particular reference to cell viability and cytotoxicity, the generation of reactive oxygen species, DNA damage, and mitochondrial dynamics were studied. Pretreatment of SH-SY5Y cells with EA (10 and 20 μM) for 60 min followed by exposure to 2 μM As O protected the SH-SY5Y cells against the harmful effects of the second. Also, EA pre-treated groups expressed improved viability, repaired DNA, reduced free radical generation, and maintained altered mitochondrial membrane potential than those exposed to As O alone. EA supplementation also inhibited As O -induced cytochrome c expression that is an important hallmark for determining mitochondrial dynamics. Thus, the current investigations are more convinced for EA as a promising candidate in modulating As O -induced mitochondria-mediated neuronal toxicity under in vitro system.

Beneficial Effects of Pomegranate on Lipid Metabolism in Metabolic Disorders

“…A study found that ellagic acid and punicalagin directly reduce ROS level produced by mitochondria . Ellagic acid was shown to exert an antioxidative effect via ameliorating mitochondrial dysfunction in concanavalin‐induced liver damage, gentamicin‐induced oxidative stress, and arsenic‐trioxide‐induced cytotoxicity in the human neuroblastoma cell line SH‐SY5Y . Cao et al .…”

Cell type-dependent effects of ellagic acid on cellular metabolism

“…Ellagic acid has been shown to specifically induce apoptosis in cancer cells [13, 14, 18, 30–34] while having cytoprotective activity in non-transformed cells [2, 4, 10, 12, 19, 35]. To determine whether EA activated apoptotic pathways, we measured caspase-3 catalytic activities in cells treated with EA for 24 hours.…”

“…In non-transformed cells, EA is protective against oxidative insults [10–12]. In contrast, EA has anti-proliferative and apoptotic effects in cancer cells.…”

Cell type-dependent effects of ellagic acid on cellular metabolism

“…Ellagic acid has been shown to specifically induce apoptosis in cancer cells [13,14,18,[30][31][32][33][34] while having cytoprotective activity in non-transformed cells [2,4,10,12,19,35]. To determine whether EA activated apoptotic pathways, we measured caspase-3 catalytic activities in cells treated with EA for 24 hours.…”

“…The number of cellular processes purported to be modulated by EA is impressive (Garcia-Nino and Zazueta, 2015). In non-transformed cells, EA is protective against oxidative insults (Sepand et al, 2016;Dhingra et al, 2017;Firdaus et al, 2018). In contrast, EA has anti-proliferative and apoptotic effects in cancer cells.…”