Ellagic acid/phospholipid-induced coagulation and dextran sulfate-induced fibrinolytic activities in β2-glycoprotein I-depleted plasma

Shiozaki, Arihiro; Niiya, Kenji; Higuchi, Fujito; Tashiro, Shigeru; Arai, Takashi; Izumi, Ryo; Sakuragawa, Nobuo

doi:10.1016/0049-3848(94)90190-2

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…In these organs the membranebinding ApoH was reported to control the interaction of some other substrates with lipids directly [20,21]. The abnormality of plasma ApoH level was shown to be associated with many diseases such as thrombocytopenia, arterial and venous thrombosis and recurrent abortion [22,23]. In addition, it has been found that, in hyperlipidaemic, diabetic or atherosclerotic patients, the concentration of plasma ApoH increased and the distribution of the protein among different types of lipoprotein Abbreviations used : ApoH, apolipoprotein H ; DPPC, 1,2-dipalmitoyl-sn-glycero-3-L-phosphocholine ; DOPC, 1,2-dioleoyl-sn-glycero-3-L-phosphocholine ; DPPS, 1,2-dipalmitoyl-sn-glycero-3-L-phosphoserine ; DOPS, 1,2-dioleoyl-sn-glycero-3-L-phosphoserine ; DPPG, 1,2-dipalmitoyl-sn-glycero-3-L-phosphoglycerol ; CL, bovine heart cardiolipin (diphosphatidylglycerol) ; SPR, surface plasmon resonance ; ∆π, surface pressure increase ; π i , initial surface pressure ; π c , critical insertion pressure.…”

Section: Introductionmentioning

confidence: 99%

The insertion of human apolipoprotein H into phospholipid membranes: a monolayer study

Wang

Cai

Sui

1998

Biochemical Journal

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Apolipoprotein H (ApoH) is a plasma glycoprotein isolated from human serum. The interactions of ApoH with lipid membrane were reported to be essential for its physiological and pathogenic roles. In this paper we studied the ability of ApoH to insert into phospholipid membranes using the monolayer approach. The results show that ApoH is surface active and can insert into the lipid monolayers. The insertion ability of ApoH is stronger when a higher content of negatively charged lipids is present in the membrane. The acidic-pH and low-ionic-strength conditions will also enhance ApoH insertion, but these factors may not have much influence on the final insertion ability of ApoH, suggesting that, in the mechanism of ApoH insertion, not only electrostatic forces, but also hydrophobic interactions, are evidently involved. Modification by heat inactivation and reduction/alkylation does not change the critical insertion pressure (pic) of ApoH, suggesting a stable domain, maybe a linear sequence motif, but not the native three-dimensional structure of ApoH, is responsible for its insertion. The extent to which insertion of ApoH into phospholipid membranes may facilitate the 'immune cleaning' of plasma liposomes is discussed.

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Section: Introductionmentioning

confidence: 99%

The insertion of human apolipoprotein H into phospholipid membranes: a monolayer study

Wang

Cai

Sui

1998

Biochemical Journal

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“…In these organs, the membrane binding ApoH was reported to control the interaction of some other substrates with lipids directly (20,21). The abnormality of the plasma ApoH level was shown to be associated with many diseases such as thrombocytopenia, arterial and venous thrombosis, and recurrent abortion (22,23). In addition, it has been found that, in hyperlipidaemia, diabetes, or atherosclerosis patients, the concentration of plasma ApoH increased and the distribution of the protein among different types of lipoprotein was perturbed (24,25).…”

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confidence: 99%

Intrinsic Fluorescence Study of the Interaction of Human Apolipoprotein H with Phospholipid Vesicles

1999

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Apolipoprotein H (ApoH) is a plasma glycoprotein with its in vivo physiological and pathogenic roles being closely related to its interaction with negatively charged membranes. In this paper, the interaction of ApoH with phospholipid vesicles was characterized by (i) detecting the wavelength shift of the fluorescence spectrum of ApoH and (ii) measuring the fluorescence quenching extent of ApoH by the membrane resident quencher 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine (DPC). The observed blue shift upon addition of DMPG vesicles indicated that the tryptophan residues of ApoH moved from a polar to a nonpolar environment. The insertion ability of ApoH into PG-containing vesicles did not depend on the PG content in a stoichiometric way as did the blue shift, indicating that the negatively charged DMPG does not serve as a specific binding site but rather provides a suitable microenvironment for ApoH interaction. The finding that the detachment effect of cations on the blue shift is remarkably different from that on the quenching extent suggests that ApoH is capable of existing in two different conformations when membrane-bound.

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“…ApoH is a 54-kDa single-chain glycoprotein consisting of five carbohydrate chains and 326 amino acid residues that can be divided into five short consensus repeat domains (Ichinose et al, 1990). ApoH has been chiefly focused on as the cofactor or autoantigen in antiphospholipid syndrome (APS), which is related to venous and arterial thrombosis, fetal loss, and thrombocytopenia (Blank et al, 1994;Shiozaka et al, 1994). Reports show that ApoH may have an important function in blood coagulation and clearance of apoptotic bodies from the circulation (Brighton et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Human Apolipoprotein H May Have Various Orientations When Attached to Lipid Layer

Xia

Sui

2002

Biophysical Journal

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Apolipoprotein H (ApoH), also known as beta(2)-glycoprotein I, is a plasma glycoprotein with its in vivo physiological and pathogenic roles being closely related to its interaction with negatively charged membranes. Although the three-dimensional crystal structure of ApoH has been recently solved, direct evidence about the spatial state of ApoH on the membrane is still lacking. In this work, the interactions of ApoH with the lipid layer are studied by a combination of lipid monolayer approach and surface concentration determination. The spatial state of the orientation of ApoH on the lipid layer is investigated by analyzing the process of membrane-attached ApoH molecules being extruded out from the phospholipid monolayer by compression. The results show that on neutral lipid layer ApoH has an upright orientation, which is not sensitive to the phase state of the lipid layer. However, on acidic lipid layer, ApoH may have two forms of orientation. One is an upright orientation in the liquid phase region, and the other is flat orientation on the condensed domain region. The variation of the spatial state of ApoH on the lipid layer may relate to a variety of its physiological functions.

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Ellagic acid/phospholipid-induced coagulation and dextran sulfate-induced fibrinolytic activities in β2-glycoprotein I-depleted plasma

Cited by 11 publications

References 21 publications

The insertion of human apolipoprotein H into phospholipid membranes: a monolayer study

The insertion of human apolipoprotein H into phospholipid membranes: a monolayer study

Intrinsic Fluorescence Study of the Interaction of Human Apolipoprotein H with Phospholipid Vesicles

Human Apolipoprotein H May Have Various Orientations When Attached to Lipid Layer

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