Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats

Berkban, Thewarid; Boonprom, Pattanapong; Bunbupha, Sarawoot; Welbat, Jariya Umka; Kukongviriyapan, Upa; Kukongviriyapan, Veerapol; Pakdeechote, Poungrat; Prachaney, Parichat

doi:10.3390/nu7075222

Cited by 75 publications

(61 citation statements)

References 57 publications

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“…One such polyphenol is ellagic acid (EA) which is found in berries, grapes, pomegranates, and nuts. It has received increasing attention in the scientific community due its potential as an anti-inflammatory [26,27] and antioxidant [28][29][30] compound. A recent study has demonstrated that EA lowered blood pressure in hypertensive rats via an antioxidant effect of EA which includes increased nitric oxide synthase (NOS) expression and decreased ROS levels in the plasma [28].…”

Section: Introductionmentioning

confidence: 99%

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Rozentsvit

Vinokur

Samuel

et al. 2017

Cell Physiol Biochem

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Background/Aims: Elevated production of reactive oxygen species (ROS) is linked to endothelial dysfunction and is one of the key contributors to the pathogenesis of diabetic vascular complications. Emerging evidence has indicated that ellagic acid (EA), a polyphenol found in fruits and nuts, possesses numerous biological activities including radical scavenging. However, whether EA exerts a vasculo-protective effect via antioxidant mechanisms in blood vessels exposed to diabetic conditions remains unknown. Accordingly, the goal of this current study was to determine whether EA decreases vascular ROS production and thus ameliorates endothelial dysfunction in the diabetic milieu. Methods: Intact rat aortas and human aortic endothelial cells (HAEC) were stimulated with 30mM high glucose (HG) with and without EA co-treatment. Endothelium-dependent vasodilation was measured using a wire myograph. Gene and protein expression of non-phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidases 4 (NOX4) were detected using RT-PCR and western blotting, respectively. Oxidative stress was determined by measuring ROS levels using dihydroethidium (DHE) staining. Results: Intact aortas exposed to HG condition displayed exacerbated ROS production and impairment of endothelium-dependent vasodilation, characterizing endothelial dysfunction. These effects were markedly reduced with EA treatment. HG enhanced ROS production in HAEC, paralleled by increased ERK1/2 activation and NOX4 expression. EA treatment blunted the increase of ROS generation, ERK1/2 activation and decreased NOX4. Conclusions: EA significantly decreases endothelial ROS levels and ameliorates the impairment of vascular relaxation induced by HG. Our results suggest that EA exerts a vasculo-protective effect under diabetic conditions via an antioxidant effect that involves inhibition of ERK1/2 and downregulation of NOX4.

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Section: Introductionmentioning

confidence: 99%

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Rozentsvit

Vinokur

Samuel

et al. 2017

Cell Physiol Biochem

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“…Nitric oxide is a potent vasodilator, it maintains vascular tone, inhibit platelet aggregation, monocyte adhesion, smooth muscle proliferation/migration and regulates BP [10,19]. Therefore loss or decrease of NO production due to inhibition of nitric oxide synthase by L-NAME plays an important role in pathogenesis of HTN.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that NO exerts direct inhibitory effects on renal tubular function through the inhibition of both the Na + /K + -ATPase and the Na + /H + exchanger therefore, reducing sodium reabsorption and increasing natriuresis/diuresis [23]. Excessive production of reactive oxygen species is proposed to be a major factor in mediating the pathogenesis of HTN [19]. In the L-NAME model, a large quantity of superoxide production suppresses nitric oxide bioavailability [19].…”

Section: Discussionmentioning

confidence: 99%

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Acute toxicity study and prevention of Nω-nitro-L-arginine methyl ester-induced hypertension by <i>Osteopermum imbricatum</i>

Tata¹,

Gwebu²,

Aremu³

et al. 2018

Trop. J. Pharm Res

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“…≪靈樞ㆍ邪氣臟腑病形≫에서는 "滎腧治外經, 合治內府"라고 하여 五輸穴의 效果가 있음을 밝히고 있다 35) . , 최근 항고혈압 효과를 관찰하기 위한 여러 연구에 활용되고 있다 46,47) . …”

Section: 항산화능(Antioxidative Effectiveness)에 미치는 효과unclassified

The Effect of Laser Acupuncture of Five-Transport-Points of the Heart Meridian in L-NAME-Induced Hypertensive Rats

et al. 2017

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Objectives : The purpose of this study is to compare the effects of laser acupuncture to the 532 nm on the five transport points with the heart meridian for treatment to hypertensive disease in rats with L-NAME induced hypertensive. Methods : Hypertensive was induced by L-NAME for 3 weeks. The laser acupuncture therapy on the five transport points of heart meridian(Laser Well Point-HT9, Laser Brook Point-HT8. Laser Stream Point-HT7, Laser River Point-HT4 and Laser Sea Point-HT3) was treated twice a week for 5 times. The hypertensive was measured using a cardiac hypertrophy, atherogenic index, TG/HDL-cholesterol ratio, TCHO, HDLC, TG, AST, ALT, antioxidative effectiveness and glutathione peroxidase quality of hypertensive rats induced by L-NAME. Results : Blood pressure were decreased significantly after the laser acupuncture of Well, Brook and Sea Point groups. Cardiac hypertrophy were decreased at the laser acupuncture of Brook and Stream Point groups. Athrogenic index was decreased at the laser acupuncture of Well, Stream, River and Sea Point groups. TG/HDL-cholesterol ratio was decreased at the laser acupuncture of all groups. Total cholesterol was decreased significantly at the laser acupuncture of Well Point group. High density lipoprotein cholesterol and total cholesterol were decreased significantly at the laser acupuncture of Well, Stream and River Point groups. Triglyceride was decreased significantly at the laser acupuncture of Stream Point group. Cu/Zn-Superoxide Dismutase (Cu/Zn-SOD) and glutathione peroxidase(GPX) were increased significantly at the laser acupuncture of Well Point group. Conclusions : The laser acupuncture treatment in five transport points of the Heart Meridian was effective for lowering blood pressure, cardiac hypertrophy, Atherogenic index and HTR, and for enhancing antioxidant activity.

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Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats

Cited by 75 publications

References 57 publications

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Ellagic Acid Reduces High Glucose-Induced Vascular Oxidative Stress Through ERK1/2/NOX4 Signaling Pathway

Acute toxicity study and prevention of Nω-nitro-L-arginine methyl ester-induced hypertension by <i>Osteopermum imbricatum</i>

The Effect of Laser Acupuncture of Five-Transport-Points of the Heart Meridian in L-NAME-Induced Hypertensive Rats

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