Ellagic acid protects Caco-2 cell monolayers against inflammation-induced permeabilization

Iglesias, Darío E.; Cremonini, Eleonora; Fraga, César G.; Oteiza, Patricia I.

doi:10.1016/j.freeradbiomed.2020.01.022

Cited by 39 publications

(22 citation statements)

References 60 publications

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“…Surprisingly, comparing the heat stress group, dietary EA at different levels markedly decreased the MDA level, indicating a reduction of oxidative damages. Endogenous antioxidant systems in cells are responsible for maintaining ROS homeostasis by scavenging excess free radicals [ 44 ]. Given that heat stress may damage the antioxidant system [ 45 ], we further detected the levels of the antioxidant enzyme, including SOD, CAT, and GSH-Px.…”

Section: Discussionmentioning

confidence: 99%

Ellagic Acid Improves Antioxidant Capacity and Intestinal Barrier Function of Heat-Stressed Broilers via Regulating Gut Microbiota

Yang

Liu

Wang

et al. 2022

Animals

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Heat stress (HS) has been revealed to damage the antioxidant system and intestinal barrier function, which greatly threatens poultry production. The present study investigated the effects of dietary ellagic acid (EA) on the antioxidant system, gut barrier function, and gut microbiota of heat-stressed broilers. Arbor Acres 14-day-old broilers numbering 360 were randomly divided into six groups, including one negative control group (NC) and five experimental groups. The broilers in the NC group were supplemented with a basal diet at a normal temperature (23 ± 2 °C). The broilers in the experimental groups were supplemented with basal diets containing EA at different doses (0, 75, 150, 300, and 600 mg/kg) at HS temperature (35 ± 2 °C). The experiment lasted for 4 weeks. Results showed that dietary EA reduced the corticosterone (CORT), LPS, and diamine oxidase (DAO) levels in the serum of heat-stressed broilers. Additionally, dietary EA improved the antioxidant enzyme activity and mRNA levels of Nrf2/HO-1 in the ileum of heat-stressed broilers. The relative abundances of Streptococcus, Ruminococcus_torques, Rothia, Neisseria, Actinomyces, and Lautropia in the cecum were significantly reduced by the EA supplementation in a dose-dependent manner. Notably, the LPS, DAO, and MDA in the serum were revealed to be positively correlated with the relative abundances of Rothia, Neisseria, Actinomyces, and Lautropia, while the GSH-px, SOD, and CAT levels in the serum were negatively correlated with the relative abundances of Ruminococcus_torques, Rothia, Neisseria, Actinomyces, Streptococcus, and Lautropia. Taken together, dietary EA improved the antioxidant capacity, intestinal barrier function, and alleviated heat-stressed injuries probably via regulating gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Ellagic Acid Improves Antioxidant Capacity and Intestinal Barrier Function of Heat-Stressed Broilers via Regulating Gut Microbiota

Yang

Liu

Wang

et al. 2022

Animals

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“…So far, the role of non-muscle MLCK and MLC2 for barrier function was predominantly discussed with respect to TNFα-caused barrier loss (Zolotarevsky et al, 2002;Wang et al, 2006;Ye and Ma, 2008). Iglesias et al, recently showed ellagic acid to inhibit TNFα-stimulated MLC2 phosphorylation (Iglesias et al, 2020). In contrast, our data show the activation of MLCK/MLC2 in a none-inflammatory state and suggest that MLC2-triggered TJ regulation is not necessarily related to barrier loss as it seems to depend on the specific type of TJ protein affected.…”

Section: Discussionmentioning

confidence: 99%

“…A very recent cell culture study reported ellagic acid to inhibit pro-inflammatory effects of tumor necrosis factor α (TNFα). This involved loss of epithelia barrier function, upregulation of interleukin-6 and -8 secretion and induction of oxidative stress by impeding TNFα-induced signaling via NF-κB, extracellular signal-regulated kinases 1/2 (ERK1/2) and myosin light chain kinase (MLCK) (Iglesias et al, 2020). Urolithin A (UroA) can be detected at relatively high amounts in the colon.…”

Section: Introductionmentioning

confidence: 99%

The Punicalagin Metabolites Ellagic Acid and Urolithin A Exert Different Strengthening and Anti-Inflammatory Effects on Tight Junction-Mediated Intestinal Barrier Function In Vitro

et al. 2021

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Scope: Ellagitannins are polyphenols found in numerous fruits, nuts and seeds. The elagitannin punicalagin and its bioactive metabolites ellagic acid and urolithins are discussed to comprise a high potential for therapeutically or preventive medical application such as in intestinal diseases. The present study characterizes effects of punicalagin, ellagic acid and urolithin A on intestinal barrier function in the absence or presence of the proinflammatory cytokine tumor necrosis factor-α (TNFα).Methods and Results: Transepithelial resistance (TER), fluorescein and ion permeability, tight junction protein expression and signalling pathways were examined in Caco-2 and HT-29/B6 intestinal epithelial cell models. Punicalagin had less or no effects on barrier function in both cell models. Ellagic acid was most effective in ileum-like Caco-2 cells, where it increased TER and reduced fluorescein and sodium permeabilities. This was paralleled by myosin light chain kinase two mediated expression down-regulation of claudin-4, -7 and -15. Urolithin A impeded the TNFα-induced barrier loss by inhibition of claudin-1 and -2 protein expression upregulation and claudin-1 delocalization in HT-29/B6.Conclusion: Ellagic acid and urolithin A affect intestinal barrier function in distinct ways. Ellagic acid acts preventive by strengthening the barrier per se, while urolithin A protects against inflammation-induced barrier dysfunction.

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“…Aslan and coauthors [38], researched the protective role of EA in rats that caused liver damage with carbon tetrachloride (CCl4) and they concluded that EA decreased MDA levels. They remarked that EA consumption has an important role in relieving intestinal inflammation and related side diseases [39]. Khan and coauthors [40], studied the effect of pine needles and vitamin E powder on blood biochemistry of female Japanese quail.…”

Section: Discussionmentioning

confidence: 99%

Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing

Gök

Beyaz

Aslan

2021

Braz. arch. biol. technol.

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In this study, the effects of Ellagic acid (EA) on protein expression in yeasts and cellular development were investigated. Four groups were formed. Groups: 1) Control group; yeast only cultivated group; 2) Ellagic Acid (EA) group: EA (10%) given group; 3) Hydrogen peroxide (H2O2) Group: The group given H2O2 (15 mM); 4) EA + H2O2 group: EA (10%) + H2O2 (15 mM) group. After sterilization, EA (10%) and H2O2 (15 mM) were added to the Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours (overnight). S. cerevisiae cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) level were analyzed by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and measured by the Bradford method. According to the obtained results, compared with the H2O2 group, cell development (1, 3, 5 and 24 hours), GSH level and total protein synthesis (24 hours) were increased with EA, while MDA level (24 hours) decreased. These results show that EA reduces oxidative damage, increases cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture.

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Ellagic acid protects Caco-2 cell monolayers against inflammation-induced permeabilization

Cited by 39 publications

References 60 publications

Ellagic Acid Improves Antioxidant Capacity and Intestinal Barrier Function of Heat-Stressed Broilers via Regulating Gut Microbiota

Ellagic Acid Improves Antioxidant Capacity and Intestinal Barrier Function of Heat-Stressed Broilers via Regulating Gut Microbiota

The Punicalagin Metabolites Ellagic Acid and Urolithin A Exert Different Strengthening and Anti-Inflammatory Effects on Tight Junction-Mediated Intestinal Barrier Function In Vitro

Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing

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