Ellagic acid regulates Wnt/β-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells

Yang, Fang; Zhou, Hong; Xia, Jianfu; Lin, Jiejun; Li, Ri-Zeng; Yang, Daping; Xu, Mai-Yu; Li, Xiaoyang

doi:10.3329/bjp.v10i1.21068

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…In the present study, we decided to determine the mRNA expression of Wnt target genes, such as anti-apoptotic survivin (BIRC5), cell-cycle regulators, namely cyclin D1 and c-myc. A number of earlier studies indicate that higher expression of these genes occurs in various cancers, including colorectal cancer [44,45]. In the present study we showed that the inhibition of growth and migration in Caco-2 cultures treated with SPF may be associated with downregulation of pro-survival genes.…”

Section: Discussionsupporting

confidence: 70%

The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA

et al. 2017

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Spirulina platensis (SP) is a blue-green microalga that has recently raised attention not only as a nutritional component, but also as a source of bioactivities that have therapeutic effects and may find application in medicine, including cancer treatment. In the present study we determined the cytotoxic effect of S. platensis filtrates (SPF) on human colon cancer cell line Caco-2. Three concentrations of SPF were tested—1.25%, 2.5%, and 5% (v/v). We have found that the highest concentration of SPF exerts the strongest anti-proliferative and pro-apoptotic effect on Caco-2 cultures. The SPF negatively affected the morphology of Caco-2 causing colony shrinking and significant inhibition of metabolic and proliferative activity of cells. The wound-healing assay showed that the SPF impaired migratory capabilities of Caco-2. This observation was consistent with lowered mRNA levels for metalloproteinases. Furthermore, SPF decreased the transcript level of pro-survival genes (cyclin D1, surviving, and c-Myc) and reduced the autocrine secretion of Wnt-10b. The cytotoxic effect of SPF involved the modulation of the Bax and Bcl-2 ratio and a decrease of mitochondrial activity, and was related with increased levels of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Moreover, the SPF also caused an increased number of cells in the apoptotic sub-G0 phase and up-regulated expression of mir-145, simultaneously decreasing expression of mir-17 and 146. Obtained results indicate that SPF can be considered as an agent with anti-cancer properties that may be used for colon cancer prevention and treatment.

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Section: Discussionsupporting

confidence: 70%

The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA

et al. 2017

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“…The induction of apoptosis was accompanied PI3K/Akt pathway. Along with production of ROS and release of cytochrome c. Recent studies showed that ellagic acid modulates Wnt/b-catenin pathway and CDK8 activity in colon cancer cells (HT 29 and HCT 116) (Fang et al 2005). Ellagic acid caused significant decrease in viability of cancer cells and markedly down-regulated the expression of CDK 8, b-catenin, survivin, c-Myc and cyclin D1.…”

Section: Ellagic Acid and Colon Cancermentioning

confidence: 99%

Honey and its Phytochemicals: Plausible Agents in Combating Colon Cancer through its Diversified Actions

Subramanian

John

Vellayappan

et al. 2016

Journal of Food Biochemistry

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Cancer is one of the leading causes of death worldwide. Among them, colorectal cancer is linked with diet as the epithelial cells of colon and rectum come in direct contact with diet. Diets rich in phenolic constituents are associated in eradicating various noncommunicable diseases including cancer. This work illustrates antiproliferative effects of honey and its phytochemicals against colorectal cancer to limelight. Honey and its phytochemicals are found to inhibit the cancer growth. Changes like activation of caspase-3, caspase-8, polyadenosine diphosphate-ribose polymerase cleavage, boost in ROS level activating mitochondrial pathway, lipid layer breakage, DNA fragmentation, increase of G0/G1 phase cells, up-regulation of Bax regulators and p53 dependent apoptosis are significant. It is high time for scientists to initiate more trails using honey and its phytochemicals against some immune-deficient animal models of colon cancer and in low-and high-risk human individuals to validate honey as a mighty sword against colon cancer. This review promulgates honey and its phytochemicals as candidates in colon cancer prevention. PRACTICAL APPLICATIONColon cancer is the third largest cause of cancer death worldwide. The standard chemotherapeutic agents are usually noneffective in the latter stages of cancer. As colorectal cancer is more prone to the diet consumed, scientists have explored many dietary compounds to treat this particular type of cancer. In our review, we highlight the effect of honey and its phenolic constituents against the colon cancer cells. Various, in vitro experiments done are enlisted. This review also emphasis the need of numerous studies that are needed to be initiated to explore the anticancer effect of crude honey specifically. As honey is one of the ingredients used in daily life, honey can also be used in chemoprevention against colon cancer.

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“…Anitha and others found that EA treatment suppressed the Wnt/β-catenin pathway in an animal model of oral oncogenesis (Anitha et al, 2013). In another in vitro study, EA inhibited the Wnt/β-catenin pathway in colon cancer cells (Fang et al, 2015). Similar to these two studies, our work showed the inhibitory effect of EA on the Wnt/β-catenin pathway, and further demonstrated that this pathway was involved in EA-induced suppression of the pathological phenotypes of ATC cells (Fig.…”

Section: Discussionmentioning

confidence: 94%

Ellagic acid inhibits cell proliferation, migration, and invasion of anaplastic thyroid cancer cells via the Wnt/β-catenin and PI3K/Akt pathways

et al. 2023

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Anaplastic thyroid cancer (ATC) is a rare but lethal human malignant cancer with no known cure. Ellagic acid (EA), a natural plant extract, has shown antitumor activity against multiple cancers; however, its effects on the malignant phenotypes of ATC cells remain unknown. This study aimed to evaluate the effects of EA on proliferation, migration, and invasion of ATC cells and further explore the associated signaling mechanisms. The normal human thyroid cell line Nthy-ori3-1 and ATC cell line BHT-101 were used. Cytotoxicity assay was performed using the Cell Counting kit-8 (CCK-8) assay. Cell proliferation, migration, and invasion assays were performed using the CCK-8 and colony formation, wound healing, and Transwell invasion assays, respectively. Western blotting was used to detect the levels of related proteins. β-catenin nuclear protein levels were measured to evaluate the Wnt/β-catenin pathway. The phosphorylation level of the Akt protein was measured and calculated to evaluate the PI3K/Akt pathway. LiCl and IGF-1 were used as pathway agonists to determine the involvement of the corresponding pathway. The results showed that EA inhibited the proliferation, migration, and invasion of ATC cells. Furthermore, both the Wnt/β-catenin and PI3K/Akt pathways were suppressed by EA treatment, and activation of these two pathways reversed the EA-induced inhibition of the pathological phenotypes of ATC cells. These findings demonstrate that EA inhibits proliferation, migration, and invasion of ATC cells by suppressing the Wnt/β-catenin and PI3K/Akt pathways, suggesting that EA is a potential drug candidate for treating ATC and provides a theoretical basis for further in vivo experiments and clinical applications.

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Ellagic acid regulates Wnt/β-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells

Cited by 9 publications

References 41 publications

The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA

The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA

Honey and its Phytochemicals: Plausible Agents in Combating Colon Cancer through its Diversified Actions

Ellagic acid inhibits cell proliferation, migration, and invasion of anaplastic thyroid cancer cells via the Wnt/β-catenin and PI3K/Akt pathways

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