Ellagitannins from <i>Rubus idaeus</i> L. Exert Geno- and Cytotoxic Effects against Human Colon Adenocarcinoma Cell Line Caco-2

Nowak, Adriana; Sójka, Michał; Klewicka, Elżbieta; Lipińska, Lidia; Klewicki, Robert; Kołodziejczyk, Krzysztof

doi:10.1021/acs.jafc.6b05387

Cited by 37 publications

(26 citation statements)

References 41 publications

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“…The effects of the three plant extracts on cell viability were evaluated on human epithelial colorectal adenocarcinoma cells (Caco-2 cells). Caco-2 cells are a valid system for evaluating the biological effects of plant extracts for human use [ 57 , 58 , 59 ]. Caco-2 cells were obtained from the ATCC cell bank (Rockville, MD, USA) and were propagated in DMEM (Gibco BRL, Life Technologies, Mumbai, India) supplemented with 10% fetal calf serum, 1% sodium pyruvate, 1% L-glutamine solution, and 1% streptomycin/penicillin in a humidified atmosphere of 5% CO 2 at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. Flowers: New Sources of Bioactive Compounds

Gupta

Rather

Jha

et al. 2020

Plants

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Artocarpus heterophyllus Lam. (AH) and Artocarpus lakoocha Roxb. (AL) are two endemic plants that grow on the Asian continent. To date, their applications have been aimed at using their fruit as a food source or for some of their therapeutic virtues. In this study, attention was given to the flowers of AH and AL. Initially, the cytotoxicity of the phytoextracts was assessed, and the content of minerals, phenols, and flavonoids was determined. Furthermore, some antioxidant components were identified by HPLC. Furthermore, the ability of AH and AL extracts to modulate the gene expression of some targets involved in the antioxidant response was studied. The results obtained highlighted the nutritional and antioxidant value of the AH and AL flower extracts. This study will contribute to enhancing the use of AH and AL flowers as potential supplements in human nutrition.

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Section: Methodsmentioning

confidence: 99%

Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. Flowers: New Sources of Bioactive Compounds

Gupta

Rather

Jha

et al. 2020

Plants

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“…The following concentrations of leaf extracts (near to or lower than the IC 50 ) were used: 0.01%, 0.02%, 0.04%, 0.08%, 0.16%, and 0.63%. The comet assay was performed under alkaline conditions (pH > 13), as described previously (Nowak et al, 2017). The cells were incubated with extracts at 37 • C for 1 h. The samples were centrifuged (182× g, 15 min, 4 • C).…”

Section: Genotoxicity Testing (Comet Assay)mentioning

confidence: 99%

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Efenberger-Szmechtyk

Nowak

2020

Antioxidants

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Aronia melanocarpa, Cornus mas, and Chaenomeles superba leaf extracts contain large amounts of bioactive compounds—mainly polyphenols, which possess many health benefits including anti-cancer properties. Here, we investigate the biological effects of A. melanocarpa, C. mas, and C. superba leaf extracts on the human colon adenocarcinoma cell line Caco-2. The antiproliferative activity of the extracts was measured using the MTT assay. The most cytotoxic extract was C. mas (IC50 = 0.60%). The extracts caused morphological changes in the Caco-2 cells, including partial detachment of cells, necrotic cells, chromatin condensation, cytoplasmic vacuolization, cell nuclei lysis, and nucleus fragmentation. The DNA damage in the Caco-2 cells after exposure to the leaf extracts was measured using the alkaline comet assay. The extracts increased DNA damage in a concentration dependent manner. However, at lower non-cyto- and non-genotoxic (IC0) concentrations the extracts induced DNA repair in Caco-2 cells after exposure to hydrogen peroxide. In conclusion, the results of these studies suggest that A. melanocarpa, C. mas and C. superba leaf extracts can show anticancer activity. However, further research is required on the mechanisms of anti-cancer activity by these extracts, with the application of more advanced and wide-ranging techniques including in vivo experiments.

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“…The cellular compatibility of AH 30S and AH 60S samples was assessed using the Caco-2 human colon epithelial cancer cells (ATCC, HTB-37) and PrestoBlue (Nowak et al, 2017). The cells (passage 25-30) were cultured in minimum essential medium (MEM), supplemented with 20% fetal bovine serum, 0.11 g/L sodium pyruvate, 1% non-essential amino acids and 1% penicillin/streptomycin.…”

Section: Cytotoxicity Assessmentmentioning

confidence: 99%

Cellulose nanocrystals from grape pomace: Production, properties and cytotoxicity assessment

Coelho

Michelin

Cerqueira

et al. 2018

Carbohydrate Polymers

123

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Cellulose nanocrystals (CNCs) were obtained from grape pomace through chemical and physical pretreatments. Bleached cellulose pulp was subjected to acid hydrolysis (AH) for 30 or 60 min and an ultrasound treatment to obtain CNCs (AH and AH). Compositional analyses of untreated (UGP) and pretreated (PGP) grape pomace showed the effectiveness of pretreatment in removing non-cellulosic components, recovering 80.1% cellulose in PGP (compared to 19.3% of UGP). Scanning and transmission electron microscopies were used to evaluate the CNCs morphology. AH in combination with ultrasound treatment led to needle-shaped structures and apparently more dispersed suspensions. Crystallinity index and thermal stability were studied by X-ray diffraction and thermogravimetric analysis, respectively. The AH sample presented high aspect ratio, crystallinity and thermal stability. CNCs toxicity was evaluated by exposing Caco-2 cells to CNCs suspension and evaluating their viability. Results showed that CNCs are non-toxic, opening the opportunity for their use on food and pharmaceutical applications.

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Ellagitannins from Rubus idaeus L. Exert Geno- and Cytotoxic Effects against Human Colon Adenocarcinoma Cell Line Caco-2

Cited by 37 publications

References 41 publications

Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. Flowers: New Sources of Bioactive Compounds

Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. Flowers: New Sources of Bioactive Compounds

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Cellulose nanocrystals from grape pomace: Production, properties and cytotoxicity assessment

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