Early damage, such as microcrack, occupies most of the fatigue life of materials, and timely detection of early damage and positioning has great engineering and economic value. In this paper, a matrix scanning and positioning imaging method based on a probabilistic algorithm is proposed. Cooperating with the single-mode (S0) lamb wave, the microcrack in the thin plate material can be efficiently positioned. Taking the S0 mode lamb wave as the fundamental wave can effectively reduce the influence of the dispersion effect on the signal analysis. Meanwhile, in order to reduce the noise interference, the signal is reconstructed by empirical mode decomposition (EMD) to achieve the filtering effect. Then, the ABAQUS finite element simulation software is used to compare the positioning results under different locations of microcrack and different arrangements of probe. Finally, the feasibility of the localization algorithm is verified by the RAM-5000 SNAP nonlinear ultrasound system. The experimental results are consistent with the simulation theory, and the microcrack is effectively positioned. This facilitates our timely remedial action or further assessment of the remaining value of the material.