Elongated mitochondrial constrictions and fission in muscle fatigue

Lavorato, Manuela; Loro, Emanuele; Debattisti, Valentina; Khurana, Tejvir S.; Franzini‐Armstrong, Clara

doi:10.1242/jcs.221028

Cited by 12 publications

(9 citation statements)

References 47 publications

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“…In rodents, it was recently shown that acute running exercise increases dynamin-related protein 1- (DRP1-) induced mitochondrial fission and mitophagy efflux [107]. In addition, using a combination of transmission electron microscopy and molecular biology, Lavorato et al [108] reported that both exercise running to exhaustion and electrical stimulation result in mitochondrial fission in a DRP1-dependent manner. Therefore, in rodents, acute exercise seems to activate DRP1 pathways, leading to mitochondrial fission and increased mitophagy efflux; however, the mechanisms are yet to be elucidated.…”

Section: Exercise Influences Mitochondria Which In Turn Influencementioning

confidence: 99%

Stay Fit, Stay Young: Mitochondria in Movement: The Role of Exercise in the New Mitochondrial Paradigm

Huertas

Casuso

Hernansanz-Agustín

et al. 2019

Oxidative Medicine and Cellular Longevity

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Skeletal muscles require the proper production and distribution of energy to sustain their work. To ensure this requirement is met, mitochondria form large networks within skeletal muscle cells, and during exercise, they can enhance their functions. In the present review, we discuss recent findings on exercise-induced mitochondrial adaptations. We emphasize the importance of mitochondrial biogenesis, morphological changes, and increases in respiratory supercomplex formation as mechanisms triggered by exercise that may increase the function of skeletal muscles. Finally, we highlight the possible effects of nutraceutical compounds on mitochondrial performance during exercise and outline the use of exercise as a therapeutic tool in noncommunicable disease prevention. The resulting picture shows that the modulation of mitochondrial activity by exercise is not only fundamental for physical performance but also a key point for whole-organism well-being.

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Section: Exercise Influences Mitochondria Which In Turn Influencementioning

confidence: 99%

Stay Fit, Stay Young: Mitochondria in Movement: The Role of Exercise in the New Mitochondrial Paradigm

Huertas

Casuso

Hernansanz-Agustín

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Frequency of extreme mitochondrial constrictions (EMCs) is higher in exercised than resting muscles (2.8.6 D). SR elements are intimately associated with constriction areas (2.8.6 E) suggesting a possible involvement, as proposed for other cells Lavorato et al, 2018..…”

supporting

confidence: 59%

Muscle: 50+ Years of Electron Microscopy

Franzini‐Armstrong

2021

Preprint

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This project was initiated with the aim of preserving for the future a portion of the extensive muscle ultrastructural image archive accumulated over a 50 year period by the author and her laboratory. Between the late 1950s and current times electron microscopy (EM) evolved. The early EM revealed the ultrastructural identification and definition of cell organelles and laid the foundation for cell biology. This continues with he discovery of new structural organizations and organelles. Currently EM images provide the essential basis for identification of structural changes associated with mutational experiments and newly discovered pathology. The most recent development of techniques allowing near atomic level of resolution brings microscopy to modern times. This evolution has been greatly enriched by the rebirth of light microscopy, fueled by dynamic views of events based on video techniques, by the availability of specific targeting of cell components and by confocal microscopy that allows 3-D structural reconstructs. Correlation between light and electron microscopy, when appropriately done, is highly revealing.The images in this publication present descriptive and structure-function correlations covering the whole animal kingdom with some areas more extensively illustrated than others. The first part classifies the images based in their site of origin, the second part identifies functional correlations, the third part introduces results of mutational experiments, the fourth part covers aging and pathology examples, the fifth part is a guide to the techniques. The images are presented with a numerical identification correlated with a very succinct description. References are limited to the directly relevant published material from the author's laboratory that are helpful in extending the limited legends and in assigning contributions. With very few exceptions, references to the literature are not given. The author asks for the forgiveness of the many friends, trainees, collaborators and competitors whose contributions and priorities are not recognized.The work has been stimulated by interactions with teachers and advisors. Keith R. Porter, father of the endoplasmic reticulum and sarcoplasmic reticulum instilled admiration for Nature's work and was always been a guiding light in the author's work. Richard J. Podolsky and Andrew F. Huxley taught the importance of structural correlations. Past and present trainees and collaborators, whose names appear in the reference list, have added depth of understanding.In the case of skeletal muscle much necessary information is gained by using the muscle bible: "Myology", A.G. Engel and C. Franzini-Armstrong, Eds. 3rd Edition, McGraw Hill, N.Y. 2004.

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“…Indeed, Loss of MACROD1 in RD cells has been shown to cause mitochondrial fragmentation [ 33 ] which might become a problem under continued muscle use and increasing energy demands [ 79 ], such as the rotarod test. Certainly, altered mitochondrial structure has previously been linked to muscle fatigue [ 80 ]. Since Macrod1 KO mice are viable, fertile and healthy it is logical that perhaps MACROD1 is only activated under certain conditions, such as stress or exercise.…”

Section: Discussionmentioning

confidence: 99%

Behavioural Characterisation of Macrod1 and Macrod2 Knockout Mice

Crawford

Oliver

Agnew

et al. 2021

Cells

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Adenosine diphosphate ribosylation (ADP-ribosylation; ADPr), the addition of ADP-ribose moieties onto proteins and nucleic acids, is a highly conserved modification involved in a wide range of cellular functions, from viral defence, DNA damage response (DDR), metabolism, carcinogenesis and neurobiology. Here we study MACROD1 and MACROD2 (mono-ADP-ribosylhydrolases 1 and 2), two of the least well-understood ADPr-mono-hydrolases. MACROD1 has been reported to be largely localized to the mitochondria, while the MACROD2 genomic locus has been associated with various neurological conditions such as autism, attention deficit hyperactivity disorder (ADHD) and schizophrenia; yet the potential significance of disrupting these proteins in the context of mammalian behaviour is unknown. Therefore, here we analysed both Macrod1 and Macrod2 gene knockout (KO) mouse models in a battery of well-defined, spontaneous behavioural testing paradigms. Loss of Macrod1 resulted in a female-specific motor-coordination defect, whereas Macrod2 disruption was associated with hyperactivity that became more pronounced with age, in combination with a bradykinesia-like gait. These data reveal new insights into the importance of ADPr-mono-hydrolases in aspects of behaviour associated with both mitochondrial and neuropsychiatric disorders.

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Elongated mitochondrial constrictions and fission in muscle fatigue

Cited by 12 publications

References 47 publications

Stay Fit, Stay Young: Mitochondria in Movement: The Role of Exercise in the New Mitochondrial Paradigm

Stay Fit, Stay Young: Mitochondria in Movement: The Role of Exercise in the New Mitochondrial Paradigm

Muscle: 50+ Years of Electron Microscopy

Behavioural Characterisation of Macrod1 and Macrod2 Knockout Mice

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